##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727494.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2603268
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68639187359888	34.0	31.0	34.0	31.0	34.0
2	32.85686606219567	34.0	31.0	34.0	31.0	34.0
3	32.948923045956086	34.0	31.0	34.0	31.0	34.0
4	36.373585047716944	37.0	37.0	37.0	35.0	37.0
5	36.23850060769771	37.0	37.0	37.0	35.0	37.0
6	36.26217969106523	37.0	37.0	37.0	35.0	37.0
7	36.23837230742282	37.0	36.0	37.0	35.0	37.0
8	36.23108915409401	37.0	36.0	37.0	35.0	37.0
9	37.96029375385093	39.0	38.0	39.0	35.0	39.0
10	37.94173861469507	39.0	38.0	39.0	35.0	39.0
11	38.01375617108957	39.0	38.0	39.0	35.0	39.0
12	37.9933034170896	39.0	38.0	39.0	35.0	39.0
13	38.017811074388035	39.0	38.0	39.0	35.0	39.0
14	39.39901577555595	40.0	39.0	41.0	37.0	41.0
15	39.422134025386555	40.0	39.0	41.0	37.0	41.0
16	39.40618176845411	40.0	39.0	41.0	37.0	41.0
17	39.42155513761933	40.0	39.0	41.0	37.0	41.0
18	39.39360334779208	40.0	39.0	41.0	37.0	41.0
19	39.43267193389232	40.0	39.0	41.0	37.0	41.0
20	39.407276546248795	40.0	39.0	41.0	37.0	41.0
21	39.386003669234206	40.0	39.0	41.0	37.0	41.0
22	39.3606897176933	40.0	39.0	41.0	37.0	41.0
23	39.32142791291561	40.0	39.0	41.0	36.0	41.0
24	39.284130562047395	40.0	39.0	41.0	36.0	41.0
25	39.22723745691953	40.0	39.0	41.0	36.0	41.0
26	39.137073094279955	40.0	39.0	41.0	36.0	41.0
27	39.0965113849208	40.0	39.0	41.0	36.0	41.0
28	39.0275069643233	40.0	39.0	41.0	36.0	41.0
29	38.9528104674586	40.0	38.0	41.0	35.0	41.0
30	38.89894586343012	40.0	38.0	41.0	35.0	41.0
31	38.83229963261562	40.0	38.0	41.0	35.0	41.0
32	38.74738290487188	40.0	38.0	41.0	35.0	41.0
33	38.70330907152087	40.0	38.0	41.0	35.0	41.0
34	38.65844315683211	40.0	38.0	41.0	35.0	41.0
35	38.64955240874163	40.0	38.0	41.0	35.0	41.0
36	38.5814387915497	40.0	38.0	41.0	35.0	41.0
37	38.5707065119688	40.0	38.0	41.0	35.0	41.0
38	38.532102342133044	40.0	38.0	41.0	35.0	41.0
39	38.47979693216373	40.0	38.0	41.0	35.0	41.0
40	38.428630859365995	40.0	38.0	41.0	34.0	41.0
41	38.37637308183406	40.0	38.0	41.0	34.0	41.0
42	38.34056424463405	40.0	38.0	41.0	34.0	41.0
43	37.26246356502673	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	2.0
13	1.0
14	2.0
15	2.0
16	7.0
17	13.0
18	23.0
19	50.0
20	97.0
21	202.0
22	453.0
23	827.0
24	1447.0
25	2312.0
26	3607.0
27	5611.0
28	8171.0
29	12029.0
30	16714.0
31	23472.0
32	32611.0
33	45454.0
34	66083.0
35	98446.0
36	153567.0
37	269745.0
38	650195.0
39	1212119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88572363659831	16.543129635519662	11.956971007210937	27.614175720671096
2	18.705565466175592	19.564946828371106	35.084055886677824	26.645431818775478
3	20.360370119403765	21.190250101026862	29.286535231870097	29.162844547699272
4	15.703300620604562	14.30855371018274	32.86634338070456	37.121802288508135
5	16.432653111396906	34.99874004520472	32.924577876730325	15.64402896666805
6	37.47666394700814	32.235712957713154	14.635834650908011	15.65178844437069
7	30.750272349984716	29.64147371688201	20.147522268164476	19.4607316649688
8	27.999806397189992	34.085656951185975	19.096573998528005	18.817962653096032
9	28.777175457924425	13.867723184858416	17.537879311695914	39.81722204552124
10	17.19688483859518	25.967553090961054	32.44510361591661	24.390458454527156
11	37.790845967453215	20.47776102959818	20.16626793706987	21.565125065878735
12	22.519348757023863	23.151592536765328	29.113137794495223	25.215920911715585
13	32.225687097909244	18.724426374848843	22.927643254555427	26.122243272686486
14	23.560347993368335	19.126613164683775	23.97167713812024	33.34136170382765
15	27.01665752431175	26.066812944345337	21.14365482155506	25.772874709787853
16	25.722361278208773	24.915068291086435	23.580015580416617	25.782554850288175
17	25.575238507906217	24.341712032721947	23.943059262434755	26.13999019693708
18	25.434146618788382	24.793490335993067	24.58966959990289	25.182693445315657
19	26.870226192616357	24.214756221795067	24.42798820559389	24.487029379994684
20	25.7801348151631	23.64232188157347	24.380931967050646	26.19661133621279
21	25.38908786955473	24.538733622508325	25.18184835368468	24.890330154252272
22	26.430932197530183	23.506838327824873	23.66379489165157	26.398434582993374
23	25.543278678952763	23.96395607367355	24.632308314011468	25.86045693336222
24	25.156111472195718	23.776192078572013	24.647904095928656	26.419792353303617
25	25.989294993830832	23.271672374876502	24.128326395899307	26.610706235393362
26	25.42949861481799	23.833811962502516	24.73644665090187	26.000242771777625
27	26.439959312679296	24.183372591680918	23.968488837876084	25.408179257763702
28	25.532599793797644	24.997464725107058	23.241671621976685	26.228263859118616
29	25.61826135457433	24.769635704045836	24.455760989648397	25.156341951731438
30	26.83991813366891	23.87184108589665	23.611091904483132	25.677148875951307
31	25.85112251216548	23.9869656139898	24.557479291413713	25.60443258243101
32	25.40729575287677	24.134895062667386	24.32984233663226	26.12796684782358
33	25.37664197462574	23.61616245426902	24.33345318269191	26.673742388413334
34	25.927872197560912	23.94006302847037	25.042292994804992	25.089771779163726
35	25.866987186874347	23.6316814096743	24.477003520190777	26.02432788326058
36	25.367883752268305	23.51893850345028	25.152846345439656	25.960331398841763
37	24.778163446867552	23.387488341576816	25.745293992013117	26.08905421954251
38	25.610886009431223	22.700390432333514	25.322594523498925	26.36612903473634
39	24.211375855271143	23.29007232447831	26.54332938445062	25.955222435799925
40	25.685715032028973	22.31564326070155	26.3877172845823	25.610924422687177
41	24.468245297833338	22.993867707819557	26.46669493882305	26.071192055524055
42	24.118415775863262	23.095048224001523	26.878907588461885	25.90762841167333
43	23.694064537343063	22.760161458597423	26.363977892402936	27.18179611165658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	115.0
2	149.0
3	345.0
4	541.0
5	541.0
6	705.0
7	869.0
8	767.5
9	666.0
10	1000.5
11	1335.0
12	1335.0
13	2175.0
14	3015.0
15	4081.5
16	5148.0
17	4915.5
18	4683.0
19	4683.0
20	5666.5
21	6650.0
22	6848.5
23	7047.0
24	9095.0
25	11143.0
26	11143.0
27	13926.0
28	16709.0
29	20155.0
30	23601.0
31	29645.0
32	35689.0
33	35689.0
34	43160.5
35	50632.0
36	59994.5
37	69357.0
38	81110.0
39	92863.0
40	92863.0
41	106561.0
42	120259.0
43	136494.0
44	152729.0
45	173733.0
46	194737.0
47	194737.0
48	217498.5
49	240260.0
50	255703.5
51	271147.0
52	273096.5
53	275046.0
54	275046.0
55	259318.0
56	243590.0
57	225075.5
58	206561.0
59	193508.0
60	180455.0
61	180455.0
62	157975.0
63	135495.0
64	113630.5
65	91766.0
66	76688.0
67	61610.0
68	61610.0
69	50480.5
70	39351.0
71	31904.5
72	24458.0
73	19724.5
74	14991.0
75	14991.0
76	12184.5
77	9378.0
78	7471.5
79	5565.0
80	4340.5
81	3116.0
82	3116.0
83	2265.0
84	1414.0
85	1068.5
86	723.0
87	509.0
88	295.0
89	295.0
90	194.5
91	94.0
92	60.0
93	26.0
94	20.5
95	15.0
96	15.0
97	10.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2603268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.36223106264737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51190073256238	49.20240186374506
2	9.656347589013805	11.657573687785781
3	3.2352729326076655	5.858648769263781
4	1.6140637010436345	3.897139442889106
5	0.964784459971744	2.9118271249232937
6	0.6299025993709394	2.2813395750114505
7	0.4272982352809129	1.805487236748155
8	0.3207679105259629	1.5489813386120617
9	0.24447676722140357	1.3281446801220675
>10	1.3201208249219019	13.785653569983047
>50	0.05032852607634678	2.022512215518004
>100	0.022764475620655013	2.569167671847404
>500	0.001335360046287278	0.5721057954161457
>1k	6.358857363173801E-4	0.5590170281346366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8413255953670544E-5
2	0.0	0.0	0.0	3.8413255953670544E-5	3.8413255953670544E-5
3	3.8413255953670544E-5	0.0	0.0	3.8413255953670544E-5	3.8413255953670544E-5
4	3.8413255953670544E-5	0.0	0.0	1.1523976786101162E-4	1.1523976786101162E-4
5	3.8413255953670544E-5	0.0	0.0	1.1523976786101162E-4	1.1523976786101162E-4
6	3.8413255953670544E-5	0.0	0.0	1.5365302381468217E-4	1.5365302381468217E-4
7	3.8413255953670544E-5	0.0	0.0	1.920662797683527E-4	1.920662797683527E-4
8	7.682651190734109E-5	0.0	0.0	1.920662797683527E-4	1.920662797683527E-4
9	7.682651190734109E-5	0.0	0.0	2.3047953572202323E-4	1.920662797683527E-4
10	1.1523976786101162E-4	0.0	0.0	2.3047953572202323E-4	1.920662797683527E-4
11	1.1523976786101162E-4	0.0	0.0	2.3047953572202323E-4	1.920662797683527E-4
12	1.1523976786101162E-4	0.0	0.0	2.3047953572202323E-4	1.920662797683527E-4
13	1.1523976786101162E-4	0.0	0.0	2.3047953572202323E-4	1.920662797683527E-4
14	1.1523976786101162E-4	0.0	0.0	2.6889279167569376E-4	2.6889279167569376E-4
15	1.1523976786101162E-4	0.0	0.0	3.457193035830349E-4	3.0730604762936435E-4
16	1.1523976786101162E-4	0.0	0.0	4.2254581549037594E-4	3.0730604762936435E-4
17	1.1523976786101162E-4	0.0	0.0	6.914386071660698E-4	3.0730604762936435E-4
18	1.1523976786101162E-4	0.0	0.0	8.450916309807519E-4	3.0730604762936435E-4
19	1.1523976786101162E-4	0.0	0.0	0.0011139844226564456	3.0730604762936435E-4
20	1.1523976786101162E-4	0.0	0.0	0.0014597037262394806	3.0730604762936435E-4
21	1.1523976786101162E-4	0.0	0.0	0.002189555589359221	3.0730604762936435E-4
22	1.1523976786101162E-4	0.0	0.0	0.0036876725715523717	3.0730604762936435E-4
23	1.1523976786101162E-4	0.0	0.0	0.004532764202533124	3.841325595367054E-4
24	1.1523976786101162E-4	0.0	0.0	0.006453427000216651	3.841325595367054E-4
25	1.1523976786101162E-4	0.0	0.0	0.008066783750270813	3.841325595367054E-4
26	1.1523976786101162E-4	0.0	0.0	0.010064273059861682	3.841325595367054E-4
27	1.1523976786101162E-4	0.0	0.0	0.020819984726889434	3.841325595367054E-4
28	1.1523976786101162E-4	0.0	0.0	0.056774792299525055	3.841325595367054E-4
29	1.1523976786101162E-4	0.0	0.0	0.10978508551559041	3.841325595367054E-4
30	1.1523976786101162E-4	0.0	0.0	0.1804654764703442	3.841325595367054E-4
31	1.5365302381468217E-4	0.0	0.0	0.48980742666525307	4.2254581549037594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1505	0.0	20.405317	37
TACTTAC	745	0.0	17.134228	31
TCGTTAC	1085	0.0	16.027649	23
GTACTTA	835	0.0	15.730538	30
GTCTTAG	405	0.0	15.074074	1
TACGGAT	1690	0.0	14.887573	27
CGTTACG	935	0.0	14.839572	24
ACGGATG	1740	0.0	14.672415	28
GTTACGG	1325	0.0	14.381133	25
TTCGTTA	155	1.2119017E-7	14.32258	22
CTCGTTA	1255	0.0	14.298804	22
TCTTATA	2560	0.0	14.019531	37
ATACCGT	185	4.9058144E-9	14.000001	6
GTATAAA	310	0.0	13.725806	1
CGTTACA	270	0.0	13.703704	24
TATACTC	190	7.1377144E-9	13.631579	5
TTACGGA	1890	0.0	13.5079365	26
CGGATGG	1955	0.0	13.3427105	29
CGACTTA	195	1.0275471E-8	13.28205	19
ATCTCGT	1400	0.0	13.214286	20
>>END_MODULE