FastQCFastQC Report
Thu 9 Feb 2017
SRR2727493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804909
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14850.019.06060637
GTTACGG12100.016.35950325
TCGTTAC8900.015.38202323
TATACTG3150.015.2698425
TACTTAC8050.014.93788831
TACGGAT14650.014.90102427
GTACTTA8200.014.66463430
TCTTATA23550.014.53290837
CGTTACG8050.014.47826124
TTACGGA15400.014.17532426
TGTTACG5750.014.15652124
ACGGATG15900.013.84591128
CTCGTTA10400.013.51923122
TATACCG1652.598881E-713.4545445
TTACGGG4550.013.41758226
GTCTTAG3000.012.951
AGTGTAC10500.012.86190527
ATCTCGT11950.012.53974820
CTATACC3100.012.5322574
TATATAC3100.012.5322573
Produced by FastQC (version 0.11.5)