FastQCFastQC Report
Thu 9 Feb 2017
SRR2727492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362975
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.15730295860158844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16720.12267282965571637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1409.458745E-1118.51
TACTTAC3150.016.44444531
GTACTAG904.44693E-516.4444451
CTTATAC7900.015.68987337
GTATTAC855.365059E-415.2352941
TCTTATA13150.015.19391637
GAATAGA1252.9595358E-614.8000011
GTCTTAG1508.103416E-814.81
CGACTTA1405.9966806E-714.53571419
CTAGTAC1152.21033E-514.478263
AGTTCGC908.2754024E-414.3888898
TGTTACG1458.912684E-714.03448324
GAATATA1356.5686363E-613.7037041
TACGGAT6500.013.66153827
CTCGTTA5200.013.51923122
CATTACG2900.013.39655124
GCTTATA1805.1595634E-813.3611121
CTATACC1409.561156E-613.2142854
AGTGTAC4350.013.183907527
GTTACGG5500.013.11818125
Produced by FastQC (version 0.11.5)