##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727492.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1362975
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939596104110493	31.0	31.0	33.0	28.0	34.0
2	31.106618243181277	31.0	31.0	34.0	28.0	34.0
3	31.20218272528843	31.0	31.0	34.0	28.0	34.0
4	35.05358865716539	35.0	35.0	37.0	32.0	37.0
5	33.87599625818522	35.0	33.0	37.0	28.0	37.0
6	33.821252774262184	35.0	33.0	37.0	29.0	37.0
7	33.83603000788716	35.0	33.0	37.0	29.0	37.0
8	33.72360828335076	35.0	33.0	37.0	29.0	37.0
9	35.270891982611566	37.0	34.0	39.0	30.0	39.0
10	34.920469561070455	37.0	33.0	39.0	29.0	39.0
11	35.13288284818137	37.0	34.0	39.0	29.0	39.0
12	35.07591628606541	37.0	34.0	39.0	29.0	39.0
13	35.22514352794439	37.0	34.0	39.0	30.0	39.0
14	36.10526311927952	38.0	34.0	40.0	30.0	41.0
15	36.03618041416754	38.0	34.0	40.0	30.0	41.0
16	35.979723766026524	38.0	34.0	40.0	29.0	41.0
17	35.98264678369009	38.0	34.0	40.0	29.0	41.0
18	35.920964801261945	38.0	34.0	40.0	29.0	41.0
19	35.979116271391625	38.0	34.0	40.0	29.0	41.0
20	35.945682789486234	38.0	34.0	40.0	29.0	41.0
21	35.89319099763385	38.0	34.0	40.0	29.0	41.0
22	35.87559859865368	38.0	34.0	40.0	29.0	41.0
23	35.77965846769016	38.0	34.0	40.0	29.0	41.0
24	35.67275481942075	38.0	34.0	40.0	28.0	41.0
25	35.56242337533704	38.0	34.0	40.0	27.0	41.0
26	35.355760010271645	38.0	34.0	40.0	27.0	41.0
27	35.219042168785194	38.0	34.0	40.0	27.0	41.0
28	35.08341092096333	38.0	33.0	40.0	27.0	40.0
29	34.90598580311451	37.0	33.0	39.0	26.0	40.0
30	34.7969573910013	37.0	33.0	39.0	26.0	40.0
31	34.66972688420551	37.0	33.0	39.0	25.0	40.0
32	34.501735541737744	37.0	33.0	39.0	25.0	40.0
33	34.42098644509254	37.0	33.0	39.0	25.0	40.0
34	34.33140593187696	37.0	33.0	39.0	25.0	40.0
35	34.32347255085383	37.0	33.0	39.0	25.0	40.0
36	34.21801793870027	37.0	32.0	39.0	25.0	40.0
37	34.163247308277846	37.0	32.0	39.0	24.0	40.0
38	34.10864616005429	37.0	32.0	39.0	24.0	40.0
39	34.01182266732699	37.0	32.0	39.0	24.0	40.0
40	33.909673324895905	37.0	32.0	39.0	24.0	40.0
41	33.84593848016288	37.0	31.0	39.0	24.0	40.0
42	33.78194024101689	36.0	31.0	39.0	24.0	40.0
43	32.62310827417964	35.0	30.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	5.0
13	3.0
14	19.0
15	37.0
16	97.0
17	199.0
18	362.0
19	617.0
20	1232.0
21	2042.0
22	3410.0
23	5389.0
24	8189.0
25	12105.0
26	17228.0
27	24029.0
28	32596.0
29	43034.0
30	56160.0
31	71781.0
32	91150.0
33	113134.0
34	138768.0
35	165440.0
36	184041.0
37	184189.0
38	149341.0
39	58373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.047286267172915	18.57092022964471	12.743667345329152	26.638126157853225
2	19.594930207817455	21.112272785634367	34.052715567050015	25.24008143949816
3	21.084832810579798	22.502246923091032	29.08424585924173	27.32867440708744
4	16.672939709092244	15.574093435316128	32.63302701810378	35.11993983748785
5	16.292962086612008	35.37834516407124	32.74418092774996	15.584511821566792
6	36.64902144206607	33.27639905354097	14.481556888424219	15.593022615968744
7	30.1222693006108	29.97457767017003	20.32634494396449	19.576808085254683
8	27.573653221812577	34.57422183092133	18.917955208276013	18.93416973899008
9	28.216218199159925	14.184486142445754	17.91991782681267	39.679377831581654
10	17.11814229901502	26.750233863423762	32.48372127148333	23.64790256607788
11	37.46077514261083	21.096351730589337	20.129349401126213	21.31352372567362
12	22.257928428621216	24.01599442396229	29.188356352831125	24.537720794585375
13	32.03169537225554	19.258827197857627	23.106733432381372	25.602743997505456
14	23.220528623048846	19.746216915203874	24.533612135218917	32.49964232652837
15	26.599607476292668	26.686916487829933	21.313230250004587	25.400245785872816
16	25.32240136466186	25.382050294392783	23.844751371081642	25.450796969863713
17	25.16561198848108	25.022615968744844	24.426566884939195	25.385205157834882
18	25.105742951998383	25.119609677360188	24.90463874979365	24.870008620847777
19	26.418679726333938	24.69223573433115	24.897595333736863	23.99148920559805
20	25.549258056824225	24.37117335240925	24.727966397035896	25.351602193730628
21	25.227241878977967	24.972211522588456	25.336855041361726	24.463691557071847
22	26.065995341073755	24.211229112786366	24.16984904345274	25.552926502687136
23	25.408536473522993	24.476677855426548	24.800895100790548	25.31389057025991
24	25.058126524697812	24.41336047983272	24.79722665492764	25.731286340541832
25	25.70480016141162	23.978723013995122	24.500082540031915	25.816394284561344
26	25.263339386269006	24.468754012362663	24.977494084631047	25.29041251673728
27	25.871494341422256	24.713806196005063	24.474770263577835	24.939929198994847
28	25.35827876520112	25.444487242979513	23.8725581907225	25.324675801096863
29	25.3804361782131	25.27515178194758	24.723710999834918	24.620701040004402
30	26.084190832553787	24.743153762908342	24.050697921825417	25.12195748271245
31	25.495698747225738	24.51541664373888	24.861938039949376	25.126946569086005
32	25.089455052367065	24.816742786918322	24.67836900896935	25.415433151745265
33	25.02547735651791	24.36435004310424	24.710798070397477	25.899374529980374
34	25.494891689135894	24.446156385847136	25.335534400851078	24.723417524165885
35	25.454685522478403	24.197068911755533	24.864432583136153	25.48381298262991
36	25.04257231423907	24.01665474421761	25.510005686091088	25.43076725545223
37	24.46031658687797	23.736752324877568	26.083530512298463	25.719400575946
38	25.157321300830905	23.32412553421743	25.781250573194665	25.737302591757
39	24.00858416331921	23.846732331847612	26.60056127221703	25.54412223261615
40	25.14895724426347	23.031456923274455	26.709660852179972	25.109924980282106
41	24.277187769401493	23.428309396724078	26.747886058071497	25.54661677580293
42	23.825381976925474	23.531319356554594	26.99690016324584	25.64639850327409
43	23.53880298611493	23.226178029677726	26.684348575725892	26.550670408481448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	68.5
2	85.0
3	205.0
4	325.0
5	325.0
6	470.5
7	616.0
8	610.5
9	605.0
10	802.5
11	1000.0
12	1000.0
13	1833.5
14	2667.0
15	3843.0
16	5019.0
17	4510.5
18	4002.0
19	4002.0
20	4625.0
21	5248.0
22	5167.0
23	5086.0
24	6348.5
25	7611.0
26	7611.0
27	9365.0
28	11119.0
29	13239.0
30	15359.0
31	18981.0
32	22603.0
33	22603.0
34	27081.0
35	31559.0
36	36584.5
37	41610.0
38	47891.5
39	54173.0
40	54173.0
41	61111.0
42	68049.0
43	76438.5
44	84828.0
45	94386.0
46	103944.0
47	103944.0
48	114420.5
49	124897.0
50	130431.5
51	135966.0
52	135735.5
53	135505.0
54	135505.0
55	128715.0
56	121925.0
57	112161.0
58	102397.0
59	95248.0
60	88099.0
61	88099.0
62	76617.5
63	65136.0
64	54859.0
65	44582.0
66	37265.5
67	29949.0
68	29949.0
69	24639.5
70	19330.0
71	15674.0
72	12018.0
73	9758.0
74	7498.0
75	7498.0
76	6064.0
77	4630.0
78	3716.5
79	2803.0
80	2140.5
81	1478.0
82	1478.0
83	1090.5
84	703.0
85	513.0
86	323.0
87	228.5
88	134.0
89	134.0
90	83.5
91	33.0
92	19.5
93	6.0
94	4.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1362975.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.56973890902691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.69941369750033	63.784532292908104
2	7.632077209592207	11.229798552864665
3	2.3633912496415403	5.216222315280209
4	1.1062695052357303	3.255518346528442
5	0.6302198442676539	2.3182554699029447
6	0.4003662795635962	1.7672905593283348
7	0.25843232894335166	1.3308959276209893
8	0.19443930666920656	1.144387922024459
9	0.1353310836441271	0.89606452409767
>10	0.5541933386705894	6.464392754774185
>50	0.015673895598241186	0.7925425444913037
>100	0.009592738192239627	1.2724354873917978
>500	2.997612405800805E-4	0.15165357949976652
>1k	2.997612405800805E-4	0.37600972328710897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2144	0.15730295860158844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1672	0.12267282965571637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.336891725820356E-5
2	0.0	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
3	0.0	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
4	0.0	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
5	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
6	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
7	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
8	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
9	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
10	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
11	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
12	7.336891725820356E-5	0.0	0.0	7.336891725820356E-5	7.336891725820356E-5
13	7.336891725820356E-5	0.0	0.0	1.4673783451640712E-4	7.336891725820356E-5
14	7.336891725820356E-5	0.0	0.0	2.2010675177461069E-4	2.9347566903281425E-4
15	7.336891725820356E-5	0.0	0.0	2.2010675177461069E-4	2.9347566903281425E-4
16	7.336891725820356E-5	0.0	0.0	2.9347566903281425E-4	4.4021350354922137E-4
17	7.336891725820356E-5	0.0	0.0	3.668445862910178E-4	4.4021350354922137E-4
18	7.336891725820356E-5	0.0	0.0	5.13582420807425E-4	4.4021350354922137E-4
19	7.336891725820356E-5	0.0	0.0	6.603202553238321E-4	4.4021350354922137E-4
20	7.336891725820356E-5	7.336891725820356E-5	0.0	8.804270070984427E-4	4.4021350354922137E-4
21	7.336891725820356E-5	7.336891725820356E-5	0.0	0.0011005337588730535	4.4021350354922137E-4
22	7.336891725820356E-5	7.336891725820356E-5	0.0	0.0014673783451640712	4.4021350354922137E-4
23	7.336891725820356E-5	7.336891725820356E-5	0.0	0.0021276986004879032	5.13582420807425E-4
24	7.336891725820356E-5	7.336891725820356E-5	0.0	0.0037418147801683817	5.13582420807425E-4
25	7.336891725820356E-5	7.336891725820356E-5	0.0	0.004915717456299639	5.13582420807425E-4
26	7.336891725820356E-5	7.336891725820356E-5	0.0	0.007556998477594967	5.13582420807425E-4
27	7.336891725820356E-5	7.336891725820356E-5	0.0	0.017975384728259873	5.869513380656285E-4
28	7.336891725820356E-5	7.336891725820356E-5	0.0	0.05172508666703351	5.869513380656285E-4
29	7.336891725820356E-5	7.336891725820356E-5	0.0	0.10550450301729672	5.869513380656285E-4
30	1.4673783451640712E-4	7.336891725820356E-5	0.0	0.17850657568920927	5.869513380656285E-4
31	1.4673783451640712E-4	7.336891725820356E-5	0.0	0.4733762541499294	5.869513380656285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATA	140	9.458745E-11	18.5	1
TACTTAC	315	0.0	16.444445	31
GTACTAG	90	4.44693E-5	16.444445	1
CTTATAC	790	0.0	15.689873	37
GTATTAC	85	5.365059E-4	15.235294	1
TCTTATA	1315	0.0	15.193916	37
GAATAGA	125	2.9595358E-6	14.800001	1
GTCTTAG	150	8.103416E-8	14.8	1
CGACTTA	140	5.9966806E-7	14.535714	19
CTAGTAC	115	2.21033E-5	14.47826	3
AGTTCGC	90	8.2754024E-4	14.388889	8
TGTTACG	145	8.912684E-7	14.034483	24
GAATATA	135	6.5686363E-6	13.703704	1
TACGGAT	650	0.0	13.661538	27
CTCGTTA	520	0.0	13.519231	22
CATTACG	290	0.0	13.396551	24
GCTTATA	180	5.1595634E-8	13.361112	1
CTATACC	140	9.561156E-6	13.214285	4
AGTGTAC	435	0.0	13.1839075	27
GTTACGG	550	0.0	13.118181	25
>>END_MODULE