FastQCFastQC Report
Thu 9 Feb 2017
SRR2727491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840836
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.4305239071590655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34220.40697591444705034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.2910198897287937No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19940.23714493670584988No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT19090.22703594993554033No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA16960.20170401838170582No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG16580.19718470664909687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15880.18885965872060664No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14240.16935526071671528No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14010.16661988782592563No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11900.14152581478433368No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG11350.1349847056976628No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA10770.12808680884262805No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10020.1191671146335314No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG9910.11785889281619721No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT9840.1170263880233482No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9700.11536137843765014No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9240.10989063265607085No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9000.10703633050915994No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8920.10608489646018962No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA8800.10465774538673417No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA8780.10441988687449157No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC8710.10358738208164256No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG8620.10251701877655095No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG8490.1009709384469742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAC551.901872E-523.5454543
GTAGCAC502.7012653E-422.23
CGCAAGA3800.021.4210532
TCGGCGC909.47075E-820.55555518
TCTAGCG1001.2885721E-820.3528
AAGACGG3850.020.181825
CAAGACG4200.019.380954
GCGCAAG4150.019.1686731
CGAACGT801.6158296E-518.54
CACGATC1002.872639E-718.526
GTCGGGA500.007033347618.52
CTAGCGG1103.8478902E-818.529
CGGTCCA1258.56744E-917.7610
CTCGGCG1054.7934736E-717.61904717
ACGGACC4450.017.4606728
ACGAACG3200.017.3437515
TAACGAA3100.017.30645213
GCAAGAC4850.017.164953
CGATCCT1201.0401891E-716.95833228
AGACGGA4650.016.7096796
Produced by FastQC (version 0.11.5)