FastQCFastQC Report
Thu 9 Feb 2017
SRR2727490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737504
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.6253223014162845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78420.451337090446986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62090.35735169530544963No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC58110.3344452732195149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53250.3064741145919664No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG52360.3013518242260162No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA38000.21870453247877414No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG29280.16851759765732913No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG19680.11326592629427042No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18280.10520839088715767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT803.383811E-416.18754
ACGGACC1751.3278623E-1015.8571438
TTAGAGT2550.015.2352954
TCTATAC1601.0973963E-815.0312513
CGCGGTC750.004105529314.810
TACGCTC750.004105529314.834
GTCCTAT2006.184564E-1114.7999991
TAACGCA1001.09387576E-414.7999994
CTTATAC4050.014.61728437
TCTAGCG1152.2110195E-514.47826128
TATACTG3200.014.4531265
CCGTGTA908.277018E-414.3888889
ACTCCGT908.277018E-414.3888886
AGAACCG1956.730261E-1014.230775
TCGTTAC2900.014.03448423
TGTGCGC1854.9021764E-914.010
TGTTACG2252.7284841E-1113.97777724
GTGTAAT2151.9826984E-1013.7674421
TACGGAT5600.013.54464327
TAATACC2051.4242687E-913.5365854
Produced by FastQC (version 0.11.5)