##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727490.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1737504
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703334783689705	31.0	31.0	34.0	30.0	34.0
2	31.91413429839586	31.0	31.0	34.0	30.0	34.0
3	32.02118153397057	33.0	31.0	34.0	30.0	34.0
4	35.69249912518187	37.0	35.0	37.0	35.0	37.0
5	35.60259717387701	37.0	35.0	37.0	33.0	37.0
6	35.62672719026834	37.0	35.0	37.0	33.0	37.0
7	35.55085628579848	37.0	35.0	37.0	33.0	37.0
8	35.371600295596444	37.0	35.0	37.0	33.0	37.0
9	37.05890231044072	39.0	37.0	39.0	33.0	39.0
10	36.923832117796564	39.0	37.0	39.0	33.0	39.0
11	36.922742623901875	39.0	37.0	39.0	33.0	39.0
12	36.72436552664051	39.0	37.0	39.0	32.0	39.0
13	36.85594623091515	39.0	37.0	39.0	33.0	39.0
14	37.92379873657845	40.0	37.0	41.0	33.0	41.0
15	37.909796466655614	40.0	37.0	41.0	33.0	41.0
16	37.81096791719616	40.0	37.0	41.0	32.0	41.0
17	37.860520608873415	40.0	37.0	41.0	33.0	41.0
18	37.705441253660425	40.0	37.0	41.0	32.0	41.0
19	37.75145553621747	40.0	37.0	41.0	32.0	41.0
20	37.64834613330387	40.0	37.0	41.0	32.0	41.0
21	37.45698484722913	39.0	36.0	41.0	32.0	41.0
22	37.24166620623607	39.0	36.0	41.0	31.0	41.0
23	37.51162587251598	39.0	37.0	41.0	32.0	41.0
24	37.43590518352764	39.0	36.0	41.0	32.0	41.0
25	37.41699990330957	39.0	36.0	41.0	31.0	41.0
26	37.25858415289979	39.0	36.0	41.0	31.0	41.0
27	37.17820678398438	39.0	36.0	41.0	31.0	41.0
28	37.03324539108975	39.0	36.0	41.0	30.0	41.0
29	36.819679264047736	39.0	36.0	41.0	30.0	41.0
30	36.70498485183344	39.0	36.0	41.0	30.0	41.0
31	36.50704861686649	39.0	35.0	41.0	30.0	41.0
32	36.35000034532295	39.0	35.0	40.0	30.0	41.0
33	36.14248254968046	39.0	35.0	40.0	29.0	41.0
34	36.11906677624915	39.0	35.0	40.0	29.0	41.0
35	35.973399773468145	38.0	35.0	40.0	27.0	41.0
36	35.76289838757206	38.0	35.0	40.0	27.0	41.0
37	35.677174268375786	38.0	35.0	40.0	26.0	41.0
38	35.61896720813304	38.0	35.0	40.0	26.0	41.0
39	35.46679834981675	38.0	34.0	40.0	25.0	41.0
40	35.233780181225484	38.0	34.0	40.0	24.0	41.0
41	35.10300810818277	38.0	34.0	40.0	24.0	41.0
42	35.0923750391366	38.0	34.0	40.0	24.0	41.0
43	34.83716756911063	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	9.0
14	18.0
15	46.0
16	64.0
17	108.0
18	191.0
19	430.0
20	856.0
21	1562.0
22	2906.0
23	4814.0
24	7434.0
25	11109.0
26	15257.0
27	20794.0
28	26945.0
29	34394.0
30	43259.0
31	53405.0
32	66116.0
33	80675.0
34	101160.0
35	125868.0
36	164227.0
37	228004.0
38	354401.0
39	393442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60772924839309	19.58689015967733	12.195655376908485	25.60972521502109
2	17.938318415382064	23.13784601359191	34.80227959187432	24.1215559791517
3	19.946428900307566	25.223136176952686	30.747612667366518	24.082822255373227
4	14.598297327660829	16.452854209256497	34.535862939020575	34.4129855240621
5	12.588747997126912	40.04721715748568	34.371316555242466	12.992718290144945
6	33.97413761349614	36.020060961010735	15.603359762049468	14.402441663443652
7	27.763216660220642	32.744787925668085	21.087663683076414	18.404331731034866
8	28.66358868814115	35.01096975891854	19.493883179549513	16.831558373390795
9	28.45190572223143	13.514789030701513	18.446979114868224	39.586326132198835
10	16.427760741845773	28.867789656887123	33.339952023132035	21.364497578135072
11	36.575628027331156	22.361560030940936	20.305967640937805	20.756844300790096
12	21.69525940659705	26.723621931230085	29.78456452474354	21.796554137429325
13	30.920619463322097	20.344701364716283	24.81548243917712	23.9191967327845
14	22.29502781000792	21.166224653295764	26.178644768587585	30.36010276810874
15	25.703537948689615	27.8365977862497	22.760638248890363	23.69922601617032
16	23.5054998434536	26.847650422675283	25.867048363629667	23.77980137024145
17	23.561096837762676	27.335188868629945	26.020026428716136	23.083687864891246
18	23.639485146509013	25.799365066209916	27.33294426948082	23.228205517800244
19	24.358102197174798	26.67780908705822	27.35458450743135	21.609504208335636
20	23.71217562664604	25.445121277418643	27.493116562609355	23.349586533325965
21	23.551082472328122	26.121839143967442	27.788885665874723	22.538192717829713
22	24.17617455844706	25.7424443339411	26.16316278984106	23.91821831777078
23	23.04029228134151	25.9194223437759	27.1887143856935	23.85157098918909
24	23.042364219017625	26.722988839162387	26.93754949628893	23.29709744553106
25	23.90100972429416	25.48748089213032	26.285004235961473	24.32650514761405
26	23.591255041715012	25.786242794261195	27.241376134961413	23.38112602906238
27	24.00005985597731	26.0206019669595	26.25444315523878	23.72489502182441
28	23.15459417647384	26.71096008987605	26.70451406155036	23.429931672099748
29	23.59229101055307	26.21375260143286	27.097721789417463	23.096234598596606
30	23.72823314363593	26.76442759268468	26.812427482181334	22.694911781498057
31	24.217555758145018	25.984573272924838	26.49254332651896	23.305327642411182
32	22.63614932685047	26.800226071421996	26.82422601617032	23.739398585557215
33	22.98279601082933	26.264227305375986	27.15544827522699	23.59752840856769
34	23.18141425861466	26.251392802548946	27.35740464482384	23.209788294012558
35	22.94895436211945	26.87550647365416	27.383591634896952	22.79194752932943
36	23.55344217912592	26.28793948100263	26.921491979299038	23.237126360572407
37	23.10774536346391	26.5299533123377	27.35947658249995	23.002824741698436
38	23.688636112492404	26.15205490174411	27.063995248356264	23.09531373740722
39	23.125702156656907	25.819163581781684	27.32085796637015	23.73427629519126
40	23.834132180415125	25.964314326758387	27.435505184448495	22.766048308377997
41	22.610595428845055	26.349291857745367	27.32943348619629	23.71067922721329
42	23.077874928633257	26.654557342026266	27.31838315192368	22.9491845774168
43	22.883573217673167	25.589754037976313	27.261232204357515	24.265440539993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	504.0
1	601.0
2	698.0
3	901.5
4	1105.0
5	1105.0
6	1670.5
7	2236.0
8	2340.0
9	2444.0
10	3233.0
11	4022.0
12	4022.0
13	7666.0
14	11310.0
15	16100.5
16	20891.0
17	17940.5
18	14990.0
19	14990.0
20	17595.0
21	20200.0
22	17662.0
23	15124.0
24	17858.5
25	20593.0
26	20593.0
27	24534.5
28	28476.0
29	32223.5
30	35971.0
31	40673.5
32	45376.0
33	45376.0
34	50329.0
35	55282.0
36	61907.0
37	68532.0
38	76105.5
39	83679.0
40	83679.0
41	90189.0
42	96699.0
43	103570.0
44	110441.0
45	121036.0
46	131631.0
47	131631.0
48	150939.0
49	170247.0
50	173128.5
51	176010.0
52	156651.5
53	137293.0
54	137293.0
55	130196.0
56	123099.0
57	114311.5
58	105524.0
59	95400.0
60	85276.0
61	85276.0
62	73113.5
63	60951.0
64	51539.5
65	42128.0
66	34248.0
67	26368.0
68	26368.0
69	21206.0
70	16044.0
71	13054.0
72	10064.0
73	8028.5
74	5993.0
75	5993.0
76	4694.0
77	3395.0
78	2716.0
79	2037.0
80	1595.5
81	1154.0
82	1154.0
83	937.0
84	720.0
85	565.0
86	410.0
87	349.0
88	288.0
89	288.0
90	214.5
91	141.0
92	105.0
93	69.0
94	67.5
95	66.0
96	66.0
97	39.5
98	13.0
99	11.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1737504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.00399390452361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.6212299779682	54.16096687703167
2	8.751204750964725	11.202241110759685
3	2.723457608079226	5.229364924401937
4	1.2233893153698823	3.13207209135173
5	0.6983059086336885	2.2347183559841706
6	0.44422787946974357	1.7059415093880548
7	0.29078007223481506	1.3027760179611825
8	0.21671866201940435	1.1096687938309173
9	0.15525569776646114	0.8943286260138424
>10	0.7946335102084142	8.830985869283223
>50	0.04802789586629524	2.1211799160556244
>100	0.028786268442030905	3.4033374022294662
>500	0.0023532676682389004	1.0351548090471074
>1k	0.0010861235391871847	1.2454414483017384
>5k	4.52551474661327E-4	1.7624105856869525
>10k+	9.051029493226539E-5	0.6294116626726074
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10865	0.6253223014162845	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7842	0.451337090446986	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6209	0.35735169530544963	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5811	0.3344452732195149	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5325	0.3064741145919664	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5236	0.3013518242260162	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3800	0.21870453247877414	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2928	0.16851759765732913	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	1968	0.11326592629427042	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	1828	0.10520839088715767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.755382433651951E-5	0.0
5	0.0	0.0	0.0	5.755382433651951E-5	0.0
6	0.0	0.0	0.0	5.755382433651951E-5	0.0
7	0.0	0.0	0.0	5.755382433651951E-5	0.0
8	0.0	0.0	0.0	5.755382433651951E-5	5.755382433651951E-5
9	0.0	0.0	0.0	1.7266147300955853E-4	1.7266147300955853E-4
10	0.0	0.0	0.0	4.028767703556366E-4	1.7266147300955853E-4
11	0.0	0.0	0.0	4.028767703556366E-4	1.7266147300955853E-4
12	0.0	0.0	0.0	4.028767703556366E-4	2.3021529734607805E-4
13	0.0	0.0	0.0	4.028767703556366E-4	2.3021529734607805E-4
14	0.0	0.0	0.0	5.755382433651951E-4	2.3021529734607805E-4
15	0.0	0.0	0.0	6.906458920382341E-4	2.3021529734607805E-4
16	0.0	0.0	0.0	8.633073650477927E-4	2.3021529734607805E-4
17	0.0	0.0	0.0	0.0013812917840764682	2.3021529734607805E-4
18	0.0	0.0	0.0	0.0014963994327495073	2.3021529734607805E-4
19	0.0	0.0	0.0	0.001899276203105144	2.3021529734607805E-4
20	0.0	0.0	0.0	0.002474814446470339	3.4532294601911706E-4
21	0.0	0.0	0.0	0.0033381218115181317	3.4532294601911706E-4
22	0.0	0.0	0.0	0.0047194135955946	4.028767703556366E-4
23	0.0	0.0	0.0	0.0073668895150744975	6.330920677017146E-4
24	0.0	0.0	0.0	0.009669042488535277	6.330920677017146E-4
25	0.0	0.0	0.0	0.01070501132659263	6.330920677017146E-4
26	0.0	0.0	0.0	0.01300716430005341	6.330920677017146E-4
27	0.0	0.0	0.0	0.02273376061292521	6.330920677017146E-4
28	0.0	0.0	0.0	0.037525093467410724	6.330920677017146E-4
29	0.0	0.0	0.0	0.056575409322798684	6.330920677017146E-4
30	0.0	0.0	0.0	0.08656095180212535	6.330920677017146E-4
31	0.0	0.0	0.0	0.17530894892903845	6.330920677017146E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	80	3.383811E-4	16.1875	4
ACGGACC	175	1.3278623E-10	15.857143	8
TTAGAGT	255	0.0	15.235295	4
TCTATAC	160	1.0973963E-8	15.031251	3
CGCGGTC	75	0.0041055293	14.8	10
TACGCTC	75	0.0041055293	14.8	34
GTCCTAT	200	6.184564E-11	14.799999	1
TAACGCA	100	1.09387576E-4	14.799999	4
CTTATAC	405	0.0	14.617284	37
TCTAGCG	115	2.2110195E-5	14.478261	28
TATACTG	320	0.0	14.453126	5
CCGTGTA	90	8.277018E-4	14.388888	9
ACTCCGT	90	8.277018E-4	14.388888	6
AGAACCG	195	6.730261E-10	14.23077	5
TCGTTAC	290	0.0	14.034484	23
TGTGCGC	185	4.9021764E-9	14.0	10
TGTTACG	225	2.7284841E-11	13.977777	24
GTGTAAT	215	1.9826984E-10	13.767442	1
TACGGAT	560	0.0	13.544643	27
TAATACC	205	1.4242687E-9	13.536585	4
>>END_MODULE