FastQCFastQC Report
Thu 9 Feb 2017
SRR2727489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093692
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59520.5442117159127067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49320.45094962750024686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39120.35768753908778705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.3082220588611785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG450.00382569220.55555522
GGTATCA24700.018.6497971
AGGTGCG609.236425E-418.57
CCGGCGT650.001579867617.07692326
ATTACAC1301.3928002E-817.0769233
ATGCGAC803.3823767E-416.187521
AGTCGGG700.002592385315.85714329
CTAATAT1302.588422E-715.6538463
ACGCGCG1802.0190782E-1015.41666621
CGCGCTA1853.0377123E-1015.024
CGGCGTA750.004104235714.827
GGTTATA908.273564E-414.3888881
TAATACC1051.6555152E-414.0952384
GTGTAAT1203.3000706E-513.8750011
ACGAACG1203.3000706E-513.87500115
GTATCAA33500.013.6402982
CAAATCG1501.305214E-613.56666713
TAGACTG1652.593988E-713.4545455
TTACACT1805.1559255E-813.3611114
CTTAAAC1254.8439633E-513.323
Produced by FastQC (version 0.11.5)