##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1093692
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757488397099	31.0	31.0	34.0	30.0	34.0
2	31.98494091572399	31.0	31.0	34.0	30.0	34.0
3	32.08283959286527	33.0	31.0	34.0	30.0	34.0
4	35.74964432399615	37.0	35.0	37.0	35.0	37.0
5	35.658043580825314	37.0	35.0	37.0	33.0	37.0
6	35.66484805594262	37.0	35.0	37.0	33.0	37.0
7	35.581688446107314	37.0	35.0	37.0	33.0	37.0
8	35.39902093093851	37.0	35.0	37.0	33.0	37.0
9	37.079241687787786	39.0	37.0	39.0	33.0	39.0
10	36.98523258833383	39.0	37.0	39.0	33.0	39.0
11	36.9543546080615	39.0	37.0	39.0	33.0	39.0
12	36.76531601218625	39.0	37.0	39.0	32.0	39.0
13	36.88466039799139	39.0	37.0	39.0	33.0	39.0
14	37.99690497873259	40.0	37.0	41.0	33.0	41.0
15	37.9730563997908	40.0	37.0	41.0	33.0	41.0
16	37.88532146161808	40.0	37.0	41.0	33.0	41.0
17	37.90853823562758	40.0	37.0	41.0	33.0	41.0
18	37.745560907458405	40.0	37.0	41.0	32.0	41.0
19	37.809088847682894	40.0	37.0	41.0	32.0	41.0
20	37.674756695669345	40.0	37.0	41.0	32.0	41.0
21	37.4880514806728	39.0	36.0	41.0	32.0	41.0
22	37.231748060697164	39.0	36.0	41.0	31.0	41.0
23	37.51176473815297	39.0	37.0	41.0	32.0	41.0
24	37.42161778636033	39.0	36.0	41.0	31.0	41.0
25	37.40710455960179	39.0	36.0	41.0	31.0	41.0
26	37.23135855432791	39.0	36.0	41.0	31.0	41.0
27	37.14365836085479	39.0	36.0	41.0	31.0	41.0
28	36.980308898666166	39.0	36.0	41.0	30.0	41.0
29	36.75275214594237	39.0	36.0	41.0	30.0	41.0
30	36.64048104950937	39.0	36.0	41.0	30.0	41.0
31	36.44492233645304	39.0	35.0	41.0	30.0	41.0
32	36.31375012343512	39.0	35.0	41.0	29.0	41.0
33	36.0740875859017	39.0	35.0	40.0	28.0	41.0
34	36.05960087483496	39.0	35.0	40.0	28.0	41.0
35	35.88227032839227	38.0	35.0	40.0	27.0	41.0
36	35.6526938114204	38.0	35.0	40.0	26.0	41.0
37	35.563605658631495	38.0	35.0	40.0	26.0	41.0
38	35.475620192887945	38.0	34.0	40.0	25.0	41.0
39	35.31503293431789	38.0	34.0	40.0	25.0	41.0
40	35.05058553962176	38.0	34.0	40.0	24.0	41.0
41	34.90958697695512	38.0	33.0	40.0	23.0	41.0
42	34.89258218950125	38.0	33.0	40.0	23.0	41.0
43	34.646882303244425	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	2.0
13	3.0
14	12.0
15	13.0
16	27.0
17	49.0
18	117.0
19	265.0
20	562.0
21	1065.0
22	1878.0
23	3182.0
24	5089.0
25	7255.0
26	10107.0
27	13489.0
28	17454.0
29	22318.0
30	27819.0
31	33934.0
32	42213.0
33	51296.0
34	63595.0
35	79142.0
36	101908.0
37	139725.0
38	207368.0
39	263799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16222848845928	21.17936311136956	12.793546994949217	24.864861405221944
2	18.813066201453427	22.93854211240459	34.747351173822246	23.50104051231974
3	19.698324574011696	25.165768790482147	31.112872728336683	24.023033907169477
4	14.657600128738254	17.059738939299184	35.03308061136042	33.24958032060215
5	12.710891183258175	39.15636212023129	34.79965109006923	13.3330956064413
6	33.24491721618152	36.77214425999276	15.463677159565947	14.519261364259773
7	26.741715217812693	32.93532365602016	21.886052014643976	18.43690911152317
8	27.114214970942456	34.71041207213731	19.92133068542149	18.25404227149874
9	26.346082809419833	14.522735834220239	20.635699995976932	38.495481360382996
10	16.837830028929535	28.550359699074328	33.70857608906346	20.90323418293267
11	34.966791381851564	23.166668495335067	21.40035768753909	20.466182435274284
12	20.654901014179494	27.342341353872936	30.004242510688567	21.998515121259
13	29.433697969812343	21.537599250977422	25.640765407445603	23.38793737176463
14	22.01378450240104	22.69542064859211	26.12298526459003	29.16780958441682
15	24.754318400427177	29.16753528415678	23.126254923689668	22.951891391726374
16	23.498754676819434	27.55007808414069	25.7904419160056	23.160725323034274
17	22.497101560585612	28.089992429312822	26.396736924106605	23.01616908599496
18	22.359311396627206	26.783866024438325	27.64416307333326	23.21265950560121
19	22.799654747406034	26.868533371369637	28.386328143572413	21.945483737651916
20	22.841805554031666	26.435870427871833	28.09145536403302	22.630868654063484
21	23.192544153198526	26.636109617698583	27.94324179019322	22.228104438909675
22	23.753031017873404	26.477929801077448	27.4715367763502	22.297502404698946
23	23.04679928169905	26.581249565691255	27.481045852031468	22.890905300578225
24	22.774876290582725	27.014826843389184	27.609144073468585	22.601152792559514
25	23.17791480599657	26.55802547700815	27.381200557378126	22.88285915961715
26	22.951068490946263	26.98867688526569	27.569187668923245	22.49106695486481
27	23.18532091301756	26.955395120381244	27.222106406556872	22.63717756004433
28	22.794351609045325	27.018758480449705	27.49759530105368	22.68929460945129
29	22.6384576279245	26.99900886172707	27.67387893483723	22.688654575511205
30	22.60545016330009	27.22375220811709	27.66354695837585	22.507250670206968
31	22.66287035106776	26.759819034975113	27.851442636500952	22.725867977456176
32	22.448916148239174	27.14475373322654	27.435969175965447	22.970360942568842
33	22.36324303368773	26.94716611258014	27.566718966582915	23.122871887149216
34	22.856800634913668	26.840554744845896	27.76970115901003	22.5329434612304
35	22.626114116222848	26.694352706246367	27.872472323103764	22.80706085442702
36	22.31578908870139	26.78898629595901	27.9655515446762	22.929673070663405
37	22.5411724690315	26.395639723066456	28.13241753619849	22.93077027170355
38	22.124053206935773	26.300640399673764	28.340702866986316	23.234603526404143
39	22.289639130577896	26.125636833770383	28.619666231443585	22.96505780420813
40	22.36616890312812	25.903362189720692	28.83800923843276	22.89245966871843
41	21.68645285875731	26.09491520464628	29.201639949821338	23.01699198677507
42	21.56768084616144	26.28628535273185	28.92779685688475	23.218236944221957
43	21.501208749812562	25.7432622712793	28.948826543487566	23.806702435420576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1342.0
1	1055.0
2	768.0
3	851.0
4	934.0
5	934.0
6	1277.5
7	1621.0
8	1747.0
9	1873.0
10	2662.5
11	3452.0
12	3452.0
13	6156.0
14	8860.0
15	10758.5
16	12657.0
17	12045.0
18	11433.0
19	11433.0
20	13332.5
21	15232.0
22	14016.5
23	12801.0
24	15182.5
25	17564.0
26	17564.0
27	20579.5
28	23595.0
29	26036.5
30	28478.0
31	31734.0
32	34990.0
33	34990.0
34	39303.0
35	43616.0
36	48124.0
37	52632.0
38	56414.0
39	60196.0
40	60196.0
41	63831.0
42	67466.0
43	69924.0
44	72382.0
45	75715.0
46	79048.0
47	79048.0
48	82291.5
49	85535.0
50	84127.0
51	82719.0
52	80838.0
53	78957.0
54	78957.0
55	75387.5
56	71818.0
57	67472.0
58	63126.0
59	57164.5
60	51203.0
61	51203.0
62	44568.5
63	37934.0
64	32585.5
65	27237.0
66	22492.0
67	17747.0
68	17747.0
69	14242.0
70	10737.0
71	8696.0
72	6655.0
73	5244.5
74	3834.0
75	3834.0
76	2983.5
77	2133.0
78	1713.5
79	1294.0
80	1007.0
81	720.0
82	720.0
83	585.0
84	450.0
85	355.0
86	260.0
87	225.5
88	191.0
89	191.0
90	144.0
91	97.0
92	73.5
93	50.0
94	41.0
95	32.0
96	32.0
97	23.0
98	14.0
99	11.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1093692.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.61341933090839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.61267801485984	60.29404674021388
2	8.102974235528299	11.281514865707566
3	2.2177000284321533	4.631450460882449
4	0.9733812362928862	2.7104158468357884
5	0.5232282751220712	1.821185466093032
6	0.3174543246169569	1.3259448610782323
7	0.21352581172334745	1.0404983308628986
8	0.15955113577343988	0.8885520095455334
9	0.117926630298888	0.7388348368749748
>10	0.6973778023356336	8.986486218249382
>50	0.0464762228542236	2.2344255139920826
>100	0.016535712758418687	2.0127792900296972
>500	6.614274466177261E-4	0.3608946449428381
>1k	3.9685646797063567E-4	1.124860446026223
>5k	1.3228548932354522E-4	0.5481104686654179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5952	0.5442117159127067	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4932	0.45094962750024686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3912	0.35768753908778705	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3371	0.3082220588611785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.143342001221551E-5	0.0
10	0.0	0.0	0.0	1.8286684002443102E-4	0.0
11	0.0	0.0	0.0	1.8286684002443102E-4	0.0
12	0.0	9.143342001221551E-5	0.0	1.8286684002443102E-4	0.0
13	0.0	9.143342001221551E-5	0.0	2.743002600366465E-4	0.0
14	0.0	9.143342001221551E-5	0.0	2.743002600366465E-4	9.143342001221551E-5
15	0.0	9.143342001221551E-5	0.0	3.6573368004886203E-4	9.143342001221551E-5
16	0.0	9.143342001221551E-5	0.0	3.6573368004886203E-4	9.143342001221551E-5
17	0.0	9.143342001221551E-5	0.0	4.571671000610775E-4	9.143342001221551E-5
18	0.0	9.143342001221551E-5	0.0	5.48600520073293E-4	9.143342001221551E-5
19	0.0	9.143342001221551E-5	0.0	5.48600520073293E-4	9.143342001221551E-5
20	0.0	9.143342001221551E-5	0.0	7.314673600977241E-4	9.143342001221551E-5
21	0.0	9.143342001221551E-5	0.0	8.229007801099395E-4	9.143342001221551E-5
22	0.0	9.143342001221551E-5	0.0	0.0019201018202565257	9.143342001221551E-5
23	0.0	9.143342001221551E-5	0.0	0.0027430026003664653	9.143342001221551E-5
24	0.0	9.143342001221551E-5	0.0	0.005120271520684068	9.143342001221551E-5
25	0.0	9.143342001221551E-5	0.0	0.007588973861013887	9.143342001221551E-5
26	0.0	9.143342001221551E-5	0.0	0.014080746681881189	9.143342001221551E-5
27	0.0	9.143342001221551E-5	0.0	0.04333944108579015	9.143342001221551E-5
28	0.0	9.143342001221551E-5	0.0	0.0748839709900045	9.143342001221551E-5
29	0.0	9.143342001221551E-5	0.0	0.11200593951496399	9.143342001221551E-5
30	0.0	9.143342001221551E-5	0.0	0.16787175914242766	9.143342001221551E-5
31	0.0	9.143342001221551E-5	0.0	0.2990787168599569	9.143342001221551E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	45	0.003825692	20.555555	22
GGTATCA	2470	0.0	18.649797	1
AGGTGCG	60	9.236425E-4	18.5	7
CCGGCGT	65	0.0015798676	17.076923	26
ATTACAC	130	1.3928002E-8	17.076923	3
ATGCGAC	80	3.3823767E-4	16.1875	21
AGTCGGG	70	0.0025923853	15.857143	29
CTAATAT	130	2.588422E-7	15.653846	3
ACGCGCG	180	2.0190782E-10	15.416666	21
CGCGCTA	185	3.0377123E-10	15.0	24
CGGCGTA	75	0.0041042357	14.8	27
GGTTATA	90	8.273564E-4	14.388888	1
TAATACC	105	1.6555152E-4	14.095238	4
GTGTAAT	120	3.3000706E-5	13.875001	1
ACGAACG	120	3.3000706E-5	13.875001	15
GTATCAA	3350	0.0	13.640298	2
CAAATCG	150	1.305214E-6	13.566667	13
TAGACTG	165	2.593988E-7	13.454545	5
TTACACT	180	5.1559255E-8	13.361111	4
CTTAAAC	125	4.8439633E-5	13.32	3
>>END_MODULE