FastQCFastQC Report
Thu 9 Feb 2017
SRR2727488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12677664
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2717862.143817662307504No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2287311.8042046231861013No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1795881.4165701189114965No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1415041.1161677734951803No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT879410.6936688020758398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT862820.6805827950638225No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC851590.6717246962847414No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG831850.6561540043970245No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG745970.5884128180081125No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA595070.46938458062936517No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT575500.4539479828460511No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC543510.4287146275528363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT519530.40979947094354296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT483020.3810007900509116No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT427500.33720723313064616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT426460.3363868927272406No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT426100.33610292874144637No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC425380.3355350007698579No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC424400.3347619876974181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423580.33411518084088676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC385030.3037073706954215No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA382890.3020193625576447No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC361910.2854705724966366No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT344600.2718166375130308No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG339980.26817243302867155No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG334310.2637000002524124No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT322530.25440806760614576No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT320140.25252286225601184No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG292790.23094948722414474No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC276040.21773727399621887No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG262070.20671789376970395No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC256060.2019772727846392No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA255970.20190628178819062No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT252180.19891677204885694No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG237820.18758976417106496No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT234050.18461603020871986No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT230010.18142932325702904No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG227450.17941002380249232No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT217480.17154579897369104No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC195890.15451584771453164No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT195030.1538374893040232No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT192420.15177875040701505No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT188360.14857626767833568No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC170870.13478035070183275No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT167690.13227200216065044No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT166670.13146743753423343No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146010.11517105990504245No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA145170.11450847727152258No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT130970.10330767560963912No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA128710.10152501280993093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTGT103000.017.87135937
TGGGCTG102350.017.40644836
CAAGACG29850.016.1758794
TACCTGT125300.015.72426237
AAGACGG30300.015.6914185
ATGTACC122300.015.21749834
GTACTCC89900.015.14571736
TGTACCT124500.015.0823335
TACTCCT91450.015.01038737
TATACTG9400.014.563835
GCGCAAG33800.014.4497031
CGCAAGA33350.014.3118442
GTACCTG136200.014.22136636
AAAAAGT45900.014.1067531
CGTACAC4100.013.9878053
AAAAGTA47700.013.8459112
CGAACGT10750.013.5953494
ACGGACC34050.013.5286358
AGGTTAT14250.013.5017541
CTCCTGT68450.013.43243237
Produced by FastQC (version 0.11.5)