FastQCFastQC Report
Thu 9 Feb 2017
SRR2727487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17226304
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC701300.40710996392493715No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG579500.3364041410159719No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA435750.2529561767863844No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG341750.19838846452494974No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT312950.18166984630016977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247510.1436814304449753No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG233570.13558915481812No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC223450.12971441813635706No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC219150.12721823555418504No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG215560.12513421335186004No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT206170.11968324720148908No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT203130.1179185041666512No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA203090.11789528386356121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203070.11788367371201623No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA202300.11743668287753427No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC201410.11692003113378238No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG196890.11429613688461553No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG192420.11170126801431114No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG189620.11007584679801309No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC187070.10859555247602737No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG180600.10483966845122436No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA180440.10474678723886446No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG180290.10465971110227709No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC180140.10457263496568968No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG178000.10333034875037617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176660.1025524685968621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG73100.019.4110814
AAGACGG74050.019.0371365
GCGCAAG83000.016.651
CGAACGA40100.016.60847916
CGCAAGA84500.016.3982242
ACGAACG40850.016.30354915
ACGGACC86600.015.851048
GACGGAC86900.015.8176077
ATAACGA43400.015.6440112
TAACGAA42900.015.61072313
CGGACCA88450.015.5404199
AACGAAC43150.015.43453114
AGACGGA91000.015.4302216
GCAAGAC99800.015.0521033
CGCTTCG38700.014.96253332
CGAACGT20450.014.8361854
TTAGAGT39800.014.8278894
CTAGCGG43000.014.79999929
TTCGGGC38750.014.75225835
TCGTTTA44300.014.65801330
Produced by FastQC (version 0.11.5)