##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17226304
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51654992272283	31.0	31.0	34.0	30.0	34.0
2	31.710674791295915	31.0	31.0	34.0	30.0	34.0
3	31.819441187151927	31.0	31.0	34.0	30.0	34.0
4	35.54045179975925	37.0	35.0	37.0	33.0	37.0
5	35.42160465762127	37.0	35.0	37.0	33.0	37.0
6	35.41397922618804	37.0	35.0	37.0	33.0	37.0
7	35.29573372210313	37.0	35.0	37.0	32.0	37.0
8	35.22736403583729	37.0	35.0	37.0	32.0	37.0
9	36.858718910336194	39.0	37.0	39.0	33.0	39.0
10	36.68578918611909	39.0	35.0	39.0	32.0	39.0
11	36.71398171076047	39.0	35.0	39.0	32.0	39.0
12	36.54760556878597	39.0	35.0	39.0	32.0	39.0
13	36.62288486259153	39.0	35.0	39.0	32.0	39.0
14	37.67228321292832	39.0	37.0	41.0	32.0	41.0
15	37.6252746381348	39.0	36.0	41.0	32.0	41.0
16	37.52920127265837	39.0	36.0	41.0	32.0	41.0
17	37.51231175300285	39.0	36.0	41.0	32.0	41.0
18	37.37268110443192	39.0	36.0	41.0	32.0	41.0
19	37.382308358194535	39.0	36.0	41.0	32.0	41.0
20	37.2965778381712	39.0	36.0	41.0	31.0	41.0
21	37.12351442305906	39.0	36.0	40.0	31.0	41.0
22	36.95617330333889	39.0	36.0	40.0	31.0	41.0
23	37.10304276529661	39.0	36.0	40.0	31.0	41.0
24	37.07427124239767	39.0	36.0	41.0	31.0	41.0
25	37.054765375091485	39.0	36.0	41.0	31.0	41.0
26	36.86401563562329	39.0	36.0	41.0	30.0	41.0
27	36.8080403666393	39.0	36.0	41.0	30.0	41.0
28	36.50580966178235	39.0	35.0	40.0	30.0	41.0
29	36.35641011559996	39.0	35.0	40.0	29.0	41.0
30	36.28470854804374	39.0	35.0	40.0	29.0	41.0
31	36.160955071964366	39.0	35.0	40.0	29.0	41.0
32	36.04241490223323	38.0	35.0	40.0	28.0	41.0
33	35.83696653675681	38.0	35.0	40.0	27.0	41.0
34	35.771542752293236	38.0	34.0	40.0	27.0	41.0
35	35.559635195106274	38.0	34.0	40.0	26.0	41.0
36	35.45638797504096	38.0	34.0	40.0	26.0	41.0
37	35.38085993373854	38.0	34.0	40.0	26.0	41.0
38	35.286274757487156	38.0	34.0	40.0	25.0	41.0
39	35.240861765820455	38.0	34.0	40.0	25.0	41.0
40	35.05369671869253	38.0	34.0	40.0	24.0	41.0
41	34.88784901276559	38.0	33.0	40.0	24.0	41.0
42	34.87980561587674	38.0	33.0	40.0	24.0	41.0
43	34.61608421632406	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	30.0
11	95.0
12	144.0
13	184.0
14	225.0
15	667.0
16	1270.0
17	1936.0
18	3044.0
19	6018.0
20	11662.0
21	20416.0
22	34702.0
23	56176.0
24	84823.0
25	123089.0
26	169610.0
27	231245.0
28	307686.0
29	393950.0
30	491043.0
31	604108.0
32	732853.0
33	886582.0
34	1084655.0
35	1323893.0
36	1667396.0
37	2188518.0
38	3266025.0
39	3534257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.866490687729645	19.48547988007178	11.404129405820308	26.243900026378263
2	18.806169913174642	22.779384364748235	33.53430892662756	24.880136795449566
3	20.174310171235803	25.161537843521163	29.37517531328833	25.288976671954703
4	14.188255356459518	16.20091576231326	35.07510955338998	34.53571932783724
5	13.507331578497627	38.269782072811445	34.49099702408596	13.731889324604976
6	34.56083789070482	36.07611359929559	15.637016506849061	13.726032003150532
7	28.424837968725036	31.202630581696454	21.81068556551655	18.561845884061956
8	27.98181200099569	33.41762690360044	20.211828375953424	18.38873271945044
9	27.74446567296154	13.42164866009563	18.51782018940337	40.31606547753947
10	16.603027555998082	26.732373932330468	33.24826962301374	23.416328888657716
11	37.573172980112275	21.763867629411394	20.417925981104247	20.245033409372084
12	21.729965986900034	26.019075246785384	29.225230206084834	23.02572856022975
13	30.276622309695682	19.38808231876089	24.96140785626447	25.37388751527896
14	22.851257007887472	21.143444351150425	24.230995807342072	31.774302833620027
15	25.711197248115443	27.579259021552154	21.606939016053587	25.102604714278815
16	25.268153865158773	26.01787940117625	24.447356786458663	24.26660994720632
17	24.671995803626825	26.64986639037602	24.567028423508606	24.111109382488547
18	25.544028481094962	23.906677834084434	26.102807659727823	24.44648602509279
19	25.088771218712964	26.11728551870442	25.028439066209447	23.765504196373175
20	24.78295982701803	24.501878058113917	25.409803519083376	25.30535859578468
21	25.455681032913386	25.565280863498057	24.976472027894086	24.002566075694475
22	25.27132343653055	25.179487137809716	24.487597571713586	25.06159185394615
23	23.73207857007516	24.684848241387126	25.344989848083486	26.238083340454228
24	24.13372015262241	26.255150263225357	24.98553375117495	24.62559583297729
25	24.999274365528436	24.754445294823544	24.291751730376987	25.954528609271033
26	24.83276737714602	25.65656568002051	25.022866193467852	24.487800749365622
27	25.94346413484866	24.73431909712031	24.270766381459424	25.051450386571606
28	24.628237142453774	25.280158761856285	25.569657890630516	24.521946205059425
29	24.596477572902465	25.000493431440663	25.588826250831286	24.814202744825586
30	24.09935990912502	26.18432833880094	25.7774505779069	23.938861174167133
31	25.527228591809365	25.150955190387908	24.094704238355483	25.227111979447248
32	24.048507445357984	25.370508961179368	24.718686028064987	25.86229756539766
33	24.155373085253807	24.916093434784386	25.3760528085421	25.55248067141971
34	25.102889162991666	25.0204280616434	25.373962981264004	24.502719794100926
35	25.279543423824403	25.208524126823722	25.841556029662545	23.670376419689333
36	24.50062416174706	25.534125021827087	25.162129961249956	24.803120855175898
37	25.168074358840993	25.19993261468043	25.42386341260435	24.208129613874224
38	24.51275677011157	24.537974019267278	26.11286205096578	24.836407159655373
39	25.553334017558267	23.949368361315347	25.052820384453916	25.444477236672476
40	25.040919979120304	24.889239154260835	25.80651659229978	24.263324274319086
41	23.84205573058504	25.191062458900067	26.185088803727137	24.78179300678776
42	25.64473493559617	25.07896064065745	26.033320902731077	23.242983521015304
43	24.78156660883263	23.623094077522374	25.954493778816396	25.6408455348286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1310.0
1	1488.5
2	1667.0
3	2786.5
4	3906.0
5	3906.0
6	6117.5
7	8329.0
8	8442.5
9	8556.0
10	11707.0
11	14858.0
12	14858.0
13	26361.0
14	37864.0
15	52133.0
16	66402.0
17	67046.5
18	67691.0
19	67691.0
20	81547.0
21	95403.0
22	101129.5
23	106856.0
24	131613.5
25	156371.0
26	156371.0
27	186852.5
28	217334.0
29	249209.5
30	281085.0
31	321255.5
32	361426.0
33	361426.0
34	424071.5
35	486717.0
36	572294.0
37	657871.0
38	749175.5
39	840480.0
40	840480.0
41	904519.0
42	968558.0
43	1007456.0
44	1046354.0
45	1149582.5
46	1252811.0
47	1252811.0
48	1517154.5
49	1781498.0
50	1752977.0
51	1724456.0
52	1524226.0
53	1323996.0
54	1323996.0
55	1284097.0
56	1244198.0
57	1202705.5
58	1161213.0
59	1095687.0
60	1030161.0
61	1030161.0
62	873141.5
63	716122.0
64	632817.0
65	549512.0
66	467501.5
67	385491.0
68	385491.0
69	309476.0
70	233461.0
71	202937.0
72	172413.0
73	130360.0
74	88307.0
75	88307.0
76	66714.5
77	45122.0
78	39163.0
79	33204.0
80	25499.5
81	17795.0
82	17795.0
83	15262.0
84	12729.0
85	11392.0
86	10055.0
87	8338.5
88	6622.0
89	6622.0
90	5078.0
91	3534.0
92	2708.0
93	1882.0
94	2003.5
95	2125.0
96	2125.0
97	1166.0
98	207.0
99	279.5
100	352.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.7226304E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.554614073600824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55193490459689	33.42747274599734
2	11.010569443626949	9.371010668082532
3	3.868978092977486	4.93928608817619
4	1.888486032330941	3.214551771569154
5	1.0954974245897815	2.3309235061020885
6	0.7056673734321275	1.8017641644441444
7	0.48151707462218973	1.434354129627757
8	0.3582697401958357	1.2196824422626418
9	0.2864418597979857	1.0970480518404981
>10	1.5605985769514832	12.098487500718608
>50	0.11143562423706155	3.246387846359077
>100	0.06290117388423007	5.103608193806706
>500	0.006870071563498786	2.0317408096732317
>1k	0.008437546807407275	7.857126039299671
>5k	0.0016329957178639438	4.731884883650766
>10k+	7.62064668336507E-4	6.094671158389646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	70130	0.40710996392493715	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	57950	0.3364041410159719	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	43575	0.2529561767863844	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	34175	0.19838846452494974	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	31295	0.18166984630016977	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24751	0.1436814304449753	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	23357	0.13558915481812	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	22345	0.12971441813635706	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	21915	0.12721823555418504	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	21556	0.12513421335186004	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	20617	0.11968324720148908	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20313	0.1179185041666512	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	20309	0.11789528386356121	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20307	0.11788367371201623	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	20230	0.11743668287753427	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	20141	0.11692003113378238	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	19689	0.11429613688461553	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	19242	0.11170126801431114	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	18962	0.11007584679801309	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	18707	0.10859555247602737	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	18060	0.10483966845122436	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	18044	0.10474678723886446	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	18029	0.10465971110227709	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	18014	0.10457263496568968	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	17800	0.10333034875037617	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17666	0.1025524685968621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.805075772493043E-6	5.805075772493043E-6
2	0.0	5.805075772493043E-6	0.0	5.805075772493043E-6	5.805075772493043E-6
3	0.0	5.805075772493043E-6	0.0	1.741522731747913E-5	5.805075772493043E-6
4	0.0	5.805075772493043E-6	0.0	1.741522731747913E-5	1.1610151544986086E-5
5	0.0	1.1610151544986086E-5	0.0	1.741522731747913E-5	1.1610151544986086E-5
6	5.805075772493043E-6	1.1610151544986086E-5	0.0	1.741522731747913E-5	1.1610151544986086E-5
7	5.805075772493043E-6	1.741522731747913E-5	0.0	4.0635530407451304E-5	1.1610151544986086E-5
8	5.805075772493043E-6	2.3220303089972172E-5	0.0	5.805075772493043E-5	3.483045463495826E-5
9	5.805075772493043E-6	4.0635530407451304E-5	0.0	7.546598504240956E-5	5.224568195243739E-5
10	5.805075772493043E-6	4.6440606179944344E-5	0.0	9.868628813238173E-5	5.224568195243739E-5
11	5.805075772493043E-6	4.6440606179944344E-5	0.0	1.1610151544986086E-4	5.224568195243739E-5
12	5.805075772493043E-6	4.6440606179944344E-5	0.0	1.219065912223539E-4	5.224568195243739E-5
13	1.1610151544986086E-5	4.6440606179944344E-5	0.0	1.219065912223539E-4	5.224568195243739E-5
14	1.1610151544986086E-5	6.385583349742347E-5	0.0	1.2771166699484694E-4	6.966090926991652E-5
15	1.741522731747913E-5	6.966090926991652E-5	0.0	1.4512689431232608E-4	7.546598504240956E-5
16	2.9025378862465215E-5	6.966090926991652E-5	0.0	2.2059287935473565E-4	7.546598504240956E-5
17	2.9025378862465215E-5	6.966090926991652E-5	0.0	3.1347409171462434E-4	8.127106081490261E-5
18	2.9025378862465215E-5	6.966090926991652E-5	0.0	3.9474515252952693E-4	8.127106081490261E-5
19	2.9025378862465215E-5	7.546598504240956E-5	0.0	5.688974257043182E-4	8.127106081490261E-5
20	2.9025378862465215E-5	7.546598504240956E-5	0.0	7.836852292865609E-4	8.127106081490261E-5
21	2.9025378862465215E-5	7.546598504240956E-5	0.0	0.0012771166699484695	8.127106081490261E-5
22	2.9025378862465215E-5	9.288121235988869E-5	0.0	0.001886649626060239	8.127106081490261E-5
23	2.9025378862465215E-5	9.288121235988869E-5	0.0	0.0026645297795743067	8.707613658739565E-5
24	3.483045463495826E-5	9.288121235988869E-5	0.0	0.0039590616768402555	8.707613658739565E-5
25	3.483045463495826E-5	9.868628813238173E-5	0.0	0.004783382436534268	8.707613658739565E-5
26	3.483045463495826E-5	9.868628813238173E-5	0.0	0.006989311230081624	8.707613658739565E-5
27	3.483045463495826E-5	9.868628813238173E-5	0.0	0.016010398980535814	8.707613658739565E-5
28	3.483045463495826E-5	9.868628813238173E-5	0.0	0.028851226589290426	8.707613658739565E-5
29	3.483045463495826E-5	9.868628813238173E-5	0.0	0.04395603374931732	9.868628813238173E-5
30	3.483045463495826E-5	1.0449136390487478E-4	0.0	0.06762332767377147	9.868628813238173E-5
31	3.483045463495826E-5	1.0449136390487478E-4	0.0	0.1325879306437411	9.868628813238173E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	7310	0.0	19.411081	4
AAGACGG	7405	0.0	19.037136	5
GCGCAAG	8300	0.0	16.65	1
CGAACGA	4010	0.0	16.608479	16
CGCAAGA	8450	0.0	16.398224	2
ACGAACG	4085	0.0	16.303549	15
ACGGACC	8660	0.0	15.85104	8
GACGGAC	8690	0.0	15.817607	7
ATAACGA	4340	0.0	15.64401	12
TAACGAA	4290	0.0	15.610723	13
CGGACCA	8845	0.0	15.540419	9
AACGAAC	4315	0.0	15.434531	14
AGACGGA	9100	0.0	15.430221	6
GCAAGAC	9980	0.0	15.052103	3
CGCTTCG	3870	0.0	14.962533	32
CGAACGT	2045	0.0	14.836185	4
TTAGAGT	3980	0.0	14.827889	4
CTAGCGG	4300	0.0	14.799999	29
TTCGGGC	3875	0.0	14.752258	35
TCGTTTA	4430	0.0	14.658013	30
>>END_MODULE