FastQCFastQC Report
Thu 9 Feb 2017
SRR2727486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273682
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70860.5563398085236346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.4804181891555349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49870.39154200184975524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29060.22815742076907736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.031.4227961
TACCGAC604.323556E-827.7500027
ATACCGA751.3740646E-824.6666666
ATAGCGG450.003826031520.5555555
AATACCG450.003826031520.5555555
ATCTCCG3600.019.0138910
GTATCAA22500.018.911112
ACTATAC1900.018.53
ACGAACG500.00703528818.529
CCCACGA3600.017.9861119
CGCTACG2600.017.78846228
TACAACG1258.580173E-917.7631
GTAATAC953.6070069E-617.5263163
ACGAGAC3400.016.8676522
GCCCACG3850.016.81818418
GAGACGC3300.016.81818224
ACGCTAT2450.016.61224432
GTCGGGT1452.9795046E-916.58620630
GACGCTA3250.016.50769226
CGAGCCC4100.016.24390215
Produced by FastQC (version 0.11.5)