##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10128293
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39730485680065	31.0	31.0	34.0	30.0	34.0
2	31.57555187236388	31.0	31.0	34.0	30.0	34.0
3	31.67474746238088	31.0	31.0	34.0	30.0	34.0
4	35.3984792896493	37.0	35.0	37.0	33.0	37.0
5	35.27651224150012	37.0	35.0	37.0	33.0	37.0
6	35.301889666896486	37.0	35.0	37.0	32.0	37.0
7	35.15790785278428	37.0	35.0	37.0	32.0	37.0
8	35.13027891274472	37.0	35.0	37.0	32.0	37.0
9	36.70121638463658	39.0	35.0	39.0	32.0	39.0
10	36.5309464289787	38.0	35.0	39.0	32.0	39.0
11	36.59159820909604	39.0	35.0	39.0	32.0	39.0
12	36.42158249173874	38.0	35.0	39.0	32.0	39.0
13	36.51514366734849	38.0	35.0	39.0	32.0	39.0
14	37.47945028841484	39.0	36.0	41.0	32.0	41.0
15	37.45097243928468	39.0	36.0	41.0	32.0	41.0
16	37.33331273098043	39.0	36.0	41.0	32.0	41.0
17	37.403203975240444	39.0	36.0	41.0	32.0	41.0
18	37.294317216138985	39.0	36.0	41.0	32.0	41.0
19	37.350737088668346	39.0	36.0	41.0	32.0	41.0
20	37.319843037716225	39.0	36.0	41.0	31.0	41.0
21	37.153524389549155	39.0	36.0	40.0	31.0	41.0
22	37.02794389933229	39.0	36.0	40.0	31.0	41.0
23	37.150399381218534	39.0	36.0	40.0	31.0	41.0
24	37.16110908323841	39.0	36.0	41.0	31.0	41.0
25	37.12814489075306	39.0	36.0	41.0	31.0	41.0
26	36.92065691622468	39.0	36.0	41.0	30.0	41.0
27	36.87539489625745	39.0	36.0	41.0	30.0	41.0
28	36.62612831204626	39.0	35.0	40.0	30.0	41.0
29	36.49552348061021	39.0	35.0	40.0	30.0	41.0
30	36.40191738133958	39.0	35.0	40.0	30.0	41.0
31	36.23001891829156	38.0	35.0	40.0	30.0	41.0
32	36.0910176078042	38.0	35.0	40.0	29.0	41.0
33	35.89303735585059	38.0	35.0	40.0	28.0	41.0
34	35.817214904821576	38.0	34.0	40.0	28.0	41.0
35	35.62291404879381	38.0	34.0	40.0	27.0	41.0
36	35.51914631616601	38.0	34.0	40.0	27.0	41.0
37	35.410673842077834	38.0	34.0	40.0	26.0	41.0
38	35.32296982324662	38.0	34.0	40.0	26.0	41.0
39	35.24640776091292	38.0	34.0	40.0	25.0	41.0
40	35.04045894011953	38.0	34.0	40.0	25.0	41.0
41	34.881556250396784	38.0	33.0	40.0	24.0	41.0
42	34.84269027367198	38.0	33.0	40.0	24.0	41.0
43	34.54038572936229	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	11.0
12	20.0
13	33.0
14	32.0
15	122.0
16	253.0
17	519.0
18	1039.0
19	2212.0
20	4567.0
21	8909.0
22	16142.0
23	27628.0
24	44307.0
25	65874.0
26	95778.0
27	132989.0
28	178463.0
29	234114.0
30	296702.0
31	368487.0
32	448715.0
33	546372.0
34	664340.0
35	808387.0
36	1015840.0
37	1349425.0
38	1907041.0
39	1909964.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.84906578038372	20.376326000837455	12.7098021354635	25.06480608331532
2	17.452239977654674	24.651952703185028	33.586459238491614	24.309348080668677
3	20.70053660572418	25.943424030090757	30.527148059401522	22.828891304783543
4	14.976018170090457	17.065649660806613	33.886085246546486	34.072246922556445
5	12.883079113133872	39.727099127167826	34.09717708601045	13.292644673687857
6	33.37554511900475	35.554954818151494	16.014643336246294	15.054856726597462
7	26.38867181271316	32.34859023134501	22.18318526132686	19.079552694614975
8	28.349851253315833	33.688016332070966	20.59738990568302	17.36474250893018
9	27.766366948507514	13.324338069603634	19.719729672117502	39.18956530977135
10	16.52880697665441	29.340956072262124	33.56723586096887	20.563001090114593
11	34.88052725172939	23.20051365022714	20.586657593732724	21.332301504310745
12	21.141134048945858	27.867104555525795	30.069410511721966	20.92235088380638
13	30.847843758074532	20.535533480320918	24.880293253759543	23.736329507845007
14	22.2438173935134	22.07697782834679	26.86583020455668	28.81337457358313
15	25.819237259427624	27.67578900017999	23.460360003408272	23.04461373698411
16	22.682361183666387	26.834610728579833	27.20598624072191	23.277041847031875
17	23.327553813855896	27.6864423254738	27.09663908814644	21.88936477252386
18	23.130077299304038	24.92184023507219	29.09972095001596	22.848361515607813
19	23.04374488376274	27.45555445522755	28.848869202342385	20.65183145866732
20	23.07044237365566	25.890335123598813	28.73140617081279	22.307816331932738
21	22.50721814623649	26.769920656916224	28.84187888324321	21.88098231360408
22	23.154474302826745	26.871487623827626	26.848857946743838	23.125180126601787
23	21.802617677036	26.65510367837897	28.0195685492116	23.522710095373426
24	22.170280816323146	28.256785225308946	27.55788166870765	22.01505228966026
25	22.95434186195048	26.241134611725787	26.71085838452738	24.093665141796354
26	22.89750108927536	26.71664415711512	28.027793034818405	22.358061718791113
27	23.022941773110237	26.513263389990794	26.948242907269766	23.515551929629208
28	22.267829337085725	27.27006416579773	28.37095056393017	22.09115593318637
29	22.86280620041304	26.40581191717104	28.128234441874856	22.603147440541065
30	21.871049741550724	28.27161497006455	28.282722468633164	21.57461281975156
31	23.578780748147786	26.773919356400928	26.80697527214112	22.840324623310167
32	20.841083487612373	28.014641756513164	28.116653023367316	23.027621732507146
33	22.040525486377614	27.504861875540133	28.22707636913743	22.227536268944824
34	21.843246438466977	27.195026842134208	28.03293704082218	22.928789678576635
35	21.335194390604617	28.462831792089744	28.804221994762592	21.39775182254305
36	22.635146909750734	27.324831538740042	27.51532760752478	22.524693943984442
37	21.66497355477374	27.527422439299492	28.21346104422532	22.594142961701444
38	22.18968191382299	27.235655603565178	28.37112828390727	22.20353419870456
39	22.253058832322488	25.87703574531266	28.228034082347342	23.641871340017513
40	21.95110271790123	27.026449570524864	28.883504851212344	22.138942860361563
41	21.524782112839745	26.488086393235267	28.775530091793357	23.21160140213163
42	21.84317732514255	27.224330891691224	28.566284565424795	22.36620721774143
43	21.726217833548063	25.78946916326374	29.153214663122405	23.331098340065797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2507.0
1	2786.5
2	3066.0
3	5411.0
4	7756.0
5	7756.0
6	13169.0
7	18582.0
8	18759.5
9	18937.0
10	25586.0
11	32235.0
12	32235.0
13	62805.0
14	93375.0
15	143040.5
16	192706.0
17	161276.5
18	129847.0
19	129847.0
20	144396.0
21	158945.0
22	137828.5
23	116712.0
24	139105.5
25	161499.0
26	161499.0
27	187572.0
28	213645.0
29	233323.0
30	253001.0
31	272955.0
32	292909.0
33	292909.0
34	319414.0
35	345919.0
36	378275.5
37	410632.0
38	445467.0
39	480302.0
40	480302.0
41	511507.0
42	542712.0
43	574420.0
44	606128.0
45	664657.5
46	723187.0
47	723187.0
48	882609.0
49	1042031.0
50	1050592.5
51	1059154.0
52	876141.0
53	693128.0
54	693128.0
55	654460.5
56	615793.0
57	580906.0
58	546019.0
59	498772.5
60	451526.0
61	451526.0
62	390098.5
63	328671.0
64	276944.5
65	225218.0
66	183915.0
67	142612.0
68	142612.0
69	115516.5
70	88421.0
71	71418.0
72	54415.0
73	43218.0
74	32021.0
75	32021.0
76	25386.0
77	18751.0
78	15097.5
79	11444.0
80	8822.5
81	6201.0
82	6201.0
83	4827.5
84	3454.0
85	2823.5
86	2193.0
87	1751.5
88	1310.0
89	1310.0
90	990.5
91	671.0
92	477.0
93	283.0
94	284.0
95	285.0
96	285.0
97	161.5
98	38.0
99	45.0
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0128293E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.505552528531204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91079850262369	37.79106329308824
2	11.985571069284926	11.62733494171288
3	4.106930020002679	5.97626729448725
4	1.895137063678703	3.676986815641349
5	1.056115063519438	2.561372234485759
6	0.6437685602243215	1.8735809828506638
7	0.43942186010811557	1.4920080082361362
8	0.3181053568270574	1.2343900876145595
9	0.22301504684907494	0.9735721262631588
>10	1.2526346251337341	10.992488601580506
>50	0.09344802631058033	3.148772822482367
>100	0.06451075239184366	6.004341444995568
>500	0.006307524550417847	2.0609127456264273
>1k	0.003233679144225117	2.84450935246981
>5k	5.935230852075658E-4	2.0507316546162357
>10k+	4.093262656603902E-4	5.691667593849205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	92466	0.912947522351496	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	54526	0.5383533039575376	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	52251	0.5158914735187855	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	50573	0.4993240223204443	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	46302	0.45715502108795625	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40160	0.39651301556935603	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	34775	0.34334512242092524	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	32673	0.3225913784287244	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21245	0.20975893963573133	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20909	0.2064415000632387	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	17885	0.1765845439108051	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	14759	0.14572050788815055	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	14532	0.14347926151030585	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	12925	0.127612816888295	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	12608	0.12448297062496118	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	12047	0.11894403133874583	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11551	0.1140468586364948	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10432	0.10299860006024707	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	10386	0.10254442678544154	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10330	0.1019915201900261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9746664121979883E-5
2	0.0	9.873332060989942E-6	0.0	0.0	1.9746664121979883E-5
3	0.0	9.873332060989942E-6	0.0	0.0	1.9746664121979883E-5
4	0.0	1.9746664121979883E-5	0.0	0.0	2.9619996182969827E-5
5	0.0	1.9746664121979883E-5	0.0	9.873332060989942E-6	2.9619996182969827E-5
6	0.0	1.9746664121979883E-5	0.0	9.873332060989942E-6	4.936666030494971E-5
7	0.0	1.9746664121979883E-5	0.0	9.873332060989942E-6	4.936666030494971E-5
8	0.0	4.936666030494971E-5	0.0	9.873332060989942E-6	4.936666030494971E-5
9	0.0	7.898665648791953E-5	0.0	5.923999236593965E-5	5.923999236593965E-5
10	0.0	7.898665648791953E-5	0.0	7.898665648791953E-5	5.923999236593965E-5
11	0.0	7.898665648791953E-5	0.0	7.898665648791953E-5	5.923999236593965E-5
12	0.0	7.898665648791953E-5	0.0	8.885998854890948E-5	6.911332442692959E-5
13	0.0	7.898665648791953E-5	0.0	9.873332060989942E-5	7.898665648791953E-5
14	0.0	9.873332060989942E-5	0.0	1.2835331679286925E-4	7.898665648791953E-5
15	0.0	9.873332060989942E-5	0.0	2.0733997328078877E-4	7.898665648791953E-5
16	0.0	9.873332060989942E-5	0.0	5.13413267171477E-4	7.898665648791953E-5
17	0.0	9.873332060989942E-5	0.0	7.701199007572154E-4	7.898665648791953E-5
18	0.0	9.873332060989942E-5	0.0	9.478398778550344E-4	8.885998854890948E-5
19	0.0	1.0860665267088936E-4	0.0	0.0014415064809045314	8.885998854890948E-5
20	0.0	1.0860665267088936E-4	0.0	0.002004286408380958	1.0860665267088936E-4
21	0.0	1.184799847318793E-4	0.0	0.0034951595495904394	1.184799847318793E-4
22	0.0	1.184799847318793E-4	0.0	0.005450079297666448	1.2835331679286925E-4
23	0.0	1.184799847318793E-4	0.0	0.008194865610621651	1.3822664885385918E-4
24	0.0	1.184799847318793E-4	0.0	0.01183812514112694	1.3822664885385918E-4
25	0.0	1.184799847318793E-4	0.0	0.013921398205995817	1.3822664885385918E-4
26	0.0	1.184799847318793E-4	0.0	0.019154264198320486	1.3822664885385918E-4
27	0.0	1.184799847318793E-4	0.0	0.03727182853023703	1.3822664885385918E-4
28	0.0	1.184799847318793E-4	0.0	0.06424577172086154	1.3822664885385918E-4
29	0.0	1.184799847318793E-4	0.0	0.09356956794200168	1.3822664885385918E-4
30	0.0	1.184799847318793E-4	0.0	0.14378533480419653	1.4809998091484914E-4
31	0.0	1.184799847318793E-4	0.0	0.2741330646733857	1.5797331297583907E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	685	0.0	13.773723	10
TATACTG	1425	0.0	13.501755	5
TATACCG	560	0.0	12.553572	5
CAAGACG	1600	0.0	12.140624	4
ATACCGA	520	0.0	12.096154	6
CTTATAC	4195	0.0	12.083433	37
TCTTATA	5965	0.0	11.816429	37
ACGGACC	1365	0.0	11.791208	8
TACCGAC	425	0.0	11.752941	7
GTATTAG	1360	0.0	11.698529	1
ATGTACC	5360	0.0	11.63153	34
GCGCAAG	1540	0.0	11.412337	1
CGAACGA	535	0.0	11.411216	16
GTACTCC	3890	0.0	11.176092	36
GTACCGT	550	0.0	11.1	6
GTGCGCC	1235	0.0	11.08502	11
TGTACCT	5780	0.0	11.010381	35
TAGTACT	825	0.0	10.987879	4
CGGACCA	1550	0.0	10.980644	9
TACACTG	3725	0.0	10.975839	5
>>END_MODULE