FastQCFastQC Report
Thu 9 Feb 2017
SRR2727483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14803775
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT357140.24124927594481815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT348840.23564259791843634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290610.1963080362947964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102900.027.4173951
GTATCAA162850.017.2674262
CGTTACG50650.015.15794724
TCGTTAC86400.015.05266323
TACTTAC57150.015.02012331
TACGGAT95400.014.07861727
GTTACGG74450.013.9153825
CTCGTTA75700.013.90554822
GTACTTA69600.013.90158130
ACGGATG99550.013.69613328
AGTCGGG66150.013.25623629
GATTTCG30850.013.132919
TTACGGA106000.013.10707526
ATTTCGT30900.013.0517820
CGGATGG104150.013.02016329
CTTATAC43700.012.95423337
TATACCG7500.012.8266665
ACTTACA60150.012.76392532
TCTTATA62550.012.65867337
GTGTACT75150.012.62874228
Produced by FastQC (version 0.11.5)