##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727483.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14803775
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42022740821176	31.0	31.0	34.0	30.0	34.0
2	31.630346381243974	31.0	31.0	34.0	30.0	34.0
3	31.718862182112332	31.0	31.0	34.0	30.0	34.0
4	35.470745738840264	37.0	35.0	37.0	33.0	37.0
5	35.33462789052117	37.0	35.0	37.0	33.0	37.0
6	35.31323206411878	37.0	35.0	37.0	32.0	37.0
7	35.211287526323524	37.0	35.0	37.0	32.0	37.0
8	35.11822106185753	37.0	35.0	37.0	32.0	37.0
9	36.68744695187545	39.0	35.0	39.0	32.0	39.0
10	36.536196071610114	38.0	35.0	39.0	32.0	39.0
11	36.57721844597071	38.0	35.0	39.0	32.0	39.0
12	36.40534323170948	38.0	35.0	39.0	32.0	39.0
13	36.49809315529316	38.0	35.0	39.0	32.0	39.0
14	37.508836563646774	39.0	36.0	41.0	32.0	41.0
15	37.485835673671076	39.0	36.0	41.0	32.0	41.0
16	37.394279364553974	39.0	36.0	41.0	32.0	41.0
17	37.39136206812114	39.0	36.0	41.0	32.0	41.0
18	37.24740290905529	39.0	36.0	41.0	31.0	41.0
19	37.31393397967748	39.0	36.0	41.0	31.0	41.0
20	37.1990472024872	39.0	36.0	40.0	31.0	41.0
21	37.026133266683665	39.0	36.0	40.0	31.0	41.0
22	36.844655028869326	39.0	36.0	40.0	30.0	41.0
23	37.03702940635075	39.0	36.0	40.0	31.0	41.0
24	37.029762003272815	39.0	36.0	41.0	31.0	41.0
25	36.969976576920416	39.0	36.0	41.0	30.0	41.0
26	36.80294283046047	39.0	36.0	40.0	30.0	41.0
27	36.69123139199292	39.0	36.0	40.0	30.0	41.0
28	36.42429893726431	39.0	35.0	40.0	30.0	41.0
29	36.35144988356011	39.0	35.0	40.0	29.0	41.0
30	36.33685374169764	39.0	35.0	40.0	30.0	41.0
31	36.17596592760968	38.0	35.0	40.0	29.0	41.0
32	36.118357378438944	38.0	35.0	40.0	29.0	41.0
33	35.91601635393675	38.0	35.0	40.0	27.0	41.0
34	35.8956960640107	38.0	35.0	40.0	27.0	41.0
35	35.698196642410466	38.0	34.0	40.0	27.0	41.0
36	35.56334752453344	38.0	34.0	40.0	26.0	41.0
37	35.54782499734021	38.0	34.0	40.0	26.0	41.0
38	35.512915388135795	38.0	34.0	40.0	26.0	41.0
39	35.45235624021576	38.0	34.0	40.0	26.0	41.0
40	35.30899044331598	38.0	34.0	40.0	25.0	41.0
41	35.15977904284549	38.0	34.0	40.0	25.0	41.0
42	35.21861572470535	38.0	34.0	40.0	25.0	41.0
43	34.97938573100442	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	13.0
12	18.0
13	28.0
14	52.0
15	140.0
16	271.0
17	646.0
18	1252.0
19	2959.0
20	6431.0
21	12971.0
22	24382.0
23	41040.0
24	65699.0
25	98652.0
26	141243.0
27	197675.0
28	262821.0
29	343021.0
30	436764.0
31	539078.0
32	657143.0
33	790766.0
34	962205.0
35	1167521.0
36	1451790.0
37	1872641.0
38	2759537.0
39	2967010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.972495191260336	19.765762449105043	12.459524682049004	26.802217677585617
2	18.81551158403853	22.084421034499645	34.99695179101276	24.103115590449057
3	19.40994780047657	24.196956519536403	31.252413658002776	25.140682021984258
4	15.070054766436265	16.243485192121604	35.02014182193393	33.6663182195082
5	13.57211927363122	38.61195539651204	34.33289144154109	13.48303388831565
6	34.453158062723865	36.00849107744477	15.510665353938439	14.027685505892922
7	27.927775178966176	32.90367490724495	21.175213754599756	17.993336159189123
8	27.33479804982175	35.25491302049646	19.900106560657672	17.510182369024115
9	28.633966674040913	13.697114418450699	18.535387088766207	39.13353181874218
10	15.84839677717339	27.75703494547843	33.97796170233606	22.416606575012114
11	36.81475164273977	21.873501860167423	21.049259394985402	20.2624871021074
12	21.16169693203254	25.185049083764106	29.987108018056208	23.66614596614715
13	30.397415524080852	20.302152660385612	24.593943098973067	24.70648871656047
14	22.232579190105227	20.95304069401217	25.36671896188641	31.447661153996194
15	25.573416240114433	28.34609415503816	22.07320092341312	24.007288681434297
16	24.113430527010845	27.06365099442541	24.73515707986645	24.087761398697292
17	23.651622643548688	26.279992772113868	25.04928641512047	25.019098169216974
18	23.366208956837024	26.723541799304567	26.247656425472556	23.66259281838585
19	24.949710462365175	25.886160793446265	26.43163652514308	22.73249221904548
20	23.886461392448886	24.96702361390929	26.193595890237457	24.952919103404366
21	24.012300916489206	26.012952777247694	27.126776784975455	22.847969521287645
22	25.035681777114284	24.702624837245903	25.682895072371743	24.57879831326807
23	23.917980380004426	25.56706650837371	26.474355358683848	24.040597752938016
24	23.47130377217973	25.104947893358283	26.657896381159535	24.76585195330245
25	24.597388166194094	24.72229549557461	25.912559465406627	24.767756872824666
26	23.901518362714917	25.33831404489733	26.692495664112702	24.06767192827505
27	25.014788457673802	25.712259204155696	25.578658146317405	23.69429419185309
28	23.661721419029945	26.824022926584608	24.999582876664906	24.51467277772055
29	23.996791358960806	26.304297383606546	26.299278393517874	23.39963286391478
30	25.522915607674395	25.468382220075625	25.2880565936729	23.720645578577084
31	24.42069674795787	25.516295674583006	26.51991130640664	23.543096271052484
32	24.221085500151144	25.811510915290185	25.64718120884707	24.3202223757116
33	23.791634228431597	25.138196169558103	26.051193023401126	25.01897657860917
34	24.519212160411787	25.60897473786247	26.7737181901238	23.09809491160194
35	24.377248370770292	25.256524096049827	26.25449927467825	24.111728258501632
36	24.041097625436755	25.300269694723138	26.814721245087824	23.843911434752286
37	23.818424692350433	25.504683771538	27.249833235103882	23.427058301007683
38	24.77293122868998	24.673915943737324	26.352649915308763	24.200502912263932
39	23.211471398342653	25.584629596167197	27.33396042563468	23.869938579855475
40	24.83569224741662	24.384746458251357	27.045837970382554	23.733723323949466
41	23.32556391866264	25.29296750322131	27.24304442616833	24.138424151947728
42	23.381238906967987	25.31713701403865	27.244348147685304	24.057275931308062
43	22.929793245303983	24.7210998545979	26.87638119331049	25.472725706787624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3161.0
1	3124.5
2	3088.0
3	4254.0
4	5420.0
5	5420.0
6	7949.5
7	10479.0
8	10239.5
9	10000.0
10	13760.5
11	17521.0
12	17521.0
13	30187.0
14	42853.0
15	59279.0
16	75705.0
17	75612.0
18	75519.0
19	75519.0
20	89913.0
21	104307.0
22	103495.0
23	102683.0
24	127497.5
25	152312.0
26	152312.0
27	184315.5
28	216319.0
29	246775.0
30	277231.0
31	318088.0
32	358945.0
33	358945.0
34	411546.0
35	464147.0
36	530571.0
37	596995.0
38	661353.5
39	725712.0
40	725712.0
41	790755.0
42	855798.0
43	921342.5
44	986887.0
45	1078581.5
46	1170276.0
47	1170276.0
48	1263928.0
49	1357580.0
50	1397195.5
51	1436811.0
52	1413232.0
53	1389653.0
54	1389653.0
55	1277286.0
56	1164919.0
57	1075474.0
58	986029.0
59	878665.0
60	771301.0
61	771301.0
62	654754.5
63	538208.0
64	453034.0
65	367860.0
66	297268.5
67	226677.0
68	226677.0
69	180597.5
70	134518.0
71	106297.0
72	78076.0
73	60939.5
74	43803.0
75	43803.0
76	33657.0
77	23511.0
78	18456.0
79	13401.0
80	10287.0
81	7173.0
82	7173.0
83	5593.0
84	4013.0
85	3142.5
86	2272.0
87	1775.5
88	1279.0
89	1279.0
90	985.5
91	692.0
92	507.5
93	323.0
94	280.0
95	237.0
96	237.0
97	137.0
98	37.0
99	40.5
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.4803775E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.95764904046377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90422104295196	31.428050181694996
2	12.277294533935619	10.302528304425499
3	4.603369034169262	5.794396270182378
4	2.231941260963321	3.745880324257168
5	1.332098186768835	2.794585410394247
6	0.8865494621615853	2.231851871423263
7	0.5792592839699667	1.7013050418169546
8	0.4283239834529548	1.4377173898665991
9	0.3379033466397064	1.2759867025116276
>10	2.086029963820351	16.556570229581816
>50	0.19678938304533827	5.738650465860912
>100	0.12178686257667046	9.621239047344478
>500	0.009666020439554184	2.7583671572992507
>1k	0.004556831684579231	3.3691203918940973
>5k	9.729388626968319E-5	0.23275473287707615
>10k+	1.1350953398129706E-4	1.0109964785696912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35714	0.24124927594481815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34884	0.23564259791843634	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29061	0.1963080362947964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3510067533450082E-5
2	0.0	0.0	0.0	6.755033766725041E-6	1.3510067533450082E-5
3	0.0	0.0	0.0	6.755033766725041E-6	1.3510067533450082E-5
4	0.0	0.0	0.0	6.755033766725041E-6	1.3510067533450082E-5
5	6.755033766725041E-6	0.0	0.0	6.755033766725041E-6	1.3510067533450082E-5
6	1.3510067533450082E-5	0.0	0.0	6.755033766725041E-6	6.0795303900525374E-5
7	1.3510067533450082E-5	0.0	0.0	6.755033766725041E-6	6.755033766725042E-5
8	1.3510067533450082E-5	0.0	0.0	1.3510067533450082E-5	1.0132550650087562E-4
9	1.3510067533450082E-5	0.0	0.0	1.3510067533450082E-5	1.5536577663467595E-4
10	1.3510067533450082E-5	0.0	0.0	2.7020135066900165E-5	1.5536577663467595E-4
11	1.3510067533450082E-5	0.0	0.0	3.377516883362521E-5	1.5536577663467595E-4
12	1.3510067533450082E-5	0.0	0.0	4.728523636707529E-5	1.8914094546830117E-4
13	1.3510067533450082E-5	0.0	0.0	5.404027013380033E-5	1.8914094546830117E-4
14	2.0265101300175123E-5	6.755033766725041E-6	0.0	6.755033766725042E-5	2.0940604676847628E-4
15	2.0265101300175123E-5	1.3510067533450082E-5	0.0	8.10604052007005E-5	2.0940604676847628E-4
16	6.0795303900525374E-5	1.3510067533450082E-5	0.0	1.2159060780105075E-4	2.904664519691768E-4
17	6.0795303900525374E-5	1.3510067533450082E-5	0.0	2.2291611430192635E-4	2.972214857359018E-4
18	6.0795303900525374E-5	1.3510067533450082E-5	0.0	2.499362493688265E-4	3.445067221029771E-4
19	6.0795303900525374E-5	1.3510067533450082E-5	0.0	2.702013506690017E-4	3.445067221029771E-4
20	6.0795303900525374E-5	2.0265101300175123E-5	0.0	3.107315532693519E-4	3.512617558697022E-4
21	6.0795303900525374E-5	2.0265101300175123E-5	0.0	4.1881209353695256E-4	3.580167896364272E-4
22	6.0795303900525374E-5	2.0265101300175123E-5	0.0	6.214631065387038E-4	3.580167896364272E-4
23	6.0795303900525374E-5	2.0265101300175123E-5	0.0	8.916644572077054E-4	4.86362431204203E-4
24	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.001459087293612609	4.86362431204203E-4
25	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.0018711443533828364	5.066275325043781E-4
26	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.0031343356677604193	5.133825662711032E-4
27	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.011733493652801397	5.201376000378282E-4
28	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.023750698723805246	5.201376000378282E-4
29	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.03891574953010296	5.201376000378282E-4
30	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.06103173008236075	5.201376000378282E-4
31	6.0795303900525374E-5	2.0265101300175123E-5	0.0	0.16343128695214565	5.268926338045533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10290	0.0	27.417395	1
GTATCAA	16285	0.0	17.267426	2
CGTTACG	5065	0.0	15.157947	24
TCGTTAC	8640	0.0	15.052663	23
TACTTAC	5715	0.0	15.020123	31
TACGGAT	9540	0.0	14.078617	27
GTTACGG	7445	0.0	13.91538	25
CTCGTTA	7570	0.0	13.905548	22
GTACTTA	6960	0.0	13.901581	30
ACGGATG	9955	0.0	13.696133	28
AGTCGGG	6615	0.0	13.256236	29
GATTTCG	3085	0.0	13.1329	19
TTACGGA	10600	0.0	13.107075	26
ATTTCGT	3090	0.0	13.05178	20
CGGATGG	10415	0.0	13.020163	29
CTTATAC	4370	0.0	12.954233	37
TATACCG	750	0.0	12.826666	5
ACTTACA	6015	0.0	12.763925	32
TCTTATA	6255	0.0	12.658673	37
GTGTACT	7515	0.0	12.628742	28
>>END_MODULE