FastQCFastQC Report
Thu 9 Feb 2017
SRR2727482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13338376
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165330.12395062187480693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.12198636475684896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140090.1050277784941735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61100.025.191491
GTATCAA107650.014.5903391
AAGACGG29250.013.97777755
CAAGACG29600.013.68754
CGAACGA22000.013.28636516
ACGAACG22950.013.13943415
ACGGACC26900.012.9293688
TACCGTC22050.012.4172337
GCGCAAG29950.012.4156921
ATACCGT24200.012.3842986
CGGACCA28400.012.37676059
TAACGAA23550.012.33333313
TATACTG22800.012.3333335
TCTAGCG12600.012.18650728
ACCGTCG22100.012.1380098
ATCGCTC30450.012.09031216
TAACGCC21150.012.0709224
CGTCGTA21900.011.99543410
TATACCG6200.011.9354835
GCGGGTA16200.011.87654323
Produced by FastQC (version 0.11.5)