##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13338376
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515727326924956	31.0	31.0	34.0	30.0	34.0
2	31.726228665318775	31.0	31.0	34.0	30.0	34.0
3	31.823547259426483	31.0	31.0	34.0	30.0	34.0
4	35.559754425876136	37.0	35.0	37.0	33.0	37.0
5	35.427821422937846	37.0	35.0	37.0	33.0	37.0
6	35.40099851736073	37.0	35.0	37.0	33.0	37.0
7	35.29078367561388	37.0	35.0	37.0	32.0	37.0
8	35.20225513210904	37.0	35.0	37.0	32.0	37.0
9	36.82470354711848	39.0	37.0	39.0	33.0	39.0
10	36.66010074989639	39.0	35.0	39.0	32.0	39.0
11	36.69153816026779	39.0	35.0	39.0	32.0	39.0
12	36.51840538908185	38.0	35.0	39.0	32.0	39.0
13	36.6001202845084	39.0	35.0	39.0	32.0	39.0
14	37.64905802625447	39.0	36.0	41.0	32.0	41.0
15	37.608037965041625	39.0	36.0	41.0	32.0	41.0
16	37.528353151837976	39.0	36.0	41.0	32.0	41.0
17	37.49298685237243	39.0	36.0	41.0	32.0	41.0
18	37.360340794111664	39.0	36.0	41.0	32.0	41.0
19	37.40318828918903	39.0	36.0	41.0	32.0	41.0
20	37.28803236615912	39.0	36.0	41.0	31.0	41.0
21	37.11565943260259	39.0	36.0	40.0	31.0	41.0
22	36.93608907111331	39.0	36.0	40.0	31.0	41.0
23	37.10670376963432	39.0	36.0	40.0	31.0	41.0
24	37.09432302703118	39.0	36.0	41.0	31.0	41.0
25	37.05289721927167	39.0	36.0	41.0	31.0	41.0
26	36.87055358163543	39.0	36.0	41.0	30.0	41.0
27	36.826927281102286	39.0	36.0	41.0	30.0	41.0
28	36.56155074650767	39.0	36.0	40.0	30.0	41.0
29	36.4282929196178	39.0	35.0	40.0	30.0	41.0
30	36.380910614605554	39.0	35.0	40.0	30.0	41.0
31	36.25079874791354	39.0	35.0	40.0	29.0	41.0
32	36.20470595520774	39.0	35.0	40.0	29.0	41.0
33	35.967832065912674	38.0	35.0	40.0	27.0	41.0
34	35.928370140412895	38.0	35.0	40.0	27.0	41.0
35	35.719077419919785	38.0	34.0	40.0	27.0	41.0
36	35.61085142599069	38.0	34.0	40.0	26.0	41.0
37	35.55655351146196	38.0	34.0	40.0	26.0	41.0
38	35.50087207018306	38.0	34.0	40.0	26.0	41.0
39	35.454449627151014	38.0	34.0	40.0	26.0	41.0
40	35.29078120154957	38.0	34.0	40.0	25.0	41.0
41	35.1600838812761	38.0	34.0	40.0	25.0	41.0
42	35.172734896662085	38.0	34.0	40.0	25.0	41.0
43	34.936530879021554	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	13.0
12	19.0
13	41.0
14	58.0
15	142.0
16	359.0
17	670.0
18	1309.0
19	3036.0
20	6629.0
21	12740.0
22	23295.0
23	38430.0
24	60260.0
25	88315.0
26	125278.0
27	172682.0
28	230330.0
29	299203.0
30	380071.0
31	469822.0
32	570994.0
33	691788.0
34	844040.0
35	1032860.0
36	1291918.0
37	1673771.0
38	2459557.0
39	2860744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.608326231019426	19.73339932837401	12.0511672485466	26.607107192059964
2	19.10120842297443	21.913814695282245	34.1244766229412	24.86050025880212
3	19.53179307585871	24.086020666983746	30.315639625093787	26.06654663206375
4	14.514060782212168	16.261372448939813	35.25794294597783	33.96662382287019
5	13.69408839576872	37.75388398107836	34.79721969151267	13.754807931640253
6	34.650357734704734	36.495454918949655	15.301682903525887	13.552504442819727
7	28.63216631469978	31.44024429960589	21.40960038913283	18.5179889965615
8	27.572532068371743	34.07212392273242	19.70349313889487	18.651850870000967
9	27.265463201817074	13.863584292420605	18.85483660079758	40.01611590496474
10	16.215977117454177	27.04948488481656	34.00398969109882	22.730548306630432
11	37.083989835044385	21.725815796465778	21.24292342636015	19.947270942129684
12	21.386726540022565	25.390662251536465	29.9135367004199	23.309074508021066
13	30.372078279994508	20.149162086898734	24.646433718767565	24.832325914339197
14	22.618188301184492	20.81942359399675	24.84237211486616	31.7200159899526
15	25.024425762176744	28.372277104798965	21.764388708190562	24.83890842483373
16	25.1368082591164	26.340463036879452	23.99504257489817	24.527686129105973
17	23.955884884336744	26.51819082023179	24.958428222446273	24.567496072985197
18	24.197578475820443	25.536347153506544	25.781279520085505	24.48479485058751
19	24.73675955753534	25.648504735509032	25.683291579124774	23.931444127830854
20	24.53962911226974	25.237690105602063	25.52942727060626	24.69325351152194
21	25.08087191424203	25.402860138295697	25.54508135023334	23.97118659722893
22	25.284022582659237	25.175276210537174	25.31540571355913	24.225295493244456
23	24.64865287948098	25.140122005857386	25.38729602464348	24.823929090018154
24	24.398584955169955	25.486580975075228	25.43532286089401	24.67951120886081
25	24.82762519215233	25.077123331955853	25.360231260537265	24.735020215354552
26	24.74890496414256	25.56436405751345	25.483844510006314	24.202886468337674
27	25.23530600726805	25.353273891814116	25.099052538330003	24.31236756258783
28	24.70093060804404	25.439693707839695	25.461083118364634	24.398292565751632
29	24.42272582509295	25.57306076841738	25.551371471309555	24.452841935180114
30	24.468338574351183	25.81703349793108	25.50400438554139	24.21062354217635
31	24.561505838491883	25.40992246732286	25.525881111763532	24.502690582421728
32	24.559106745828725	25.54996950153452	25.129093676771447	24.76183007586531
33	24.29040836755539	25.29184962247278	25.527350556019712	24.890391453952116
34	25.032575180066896	25.235440956230352	25.6716484825439	24.060335381158847
35	24.707850490944324	25.105897449584567	25.955491133253407	24.230760926217705
36	24.277363301199486	25.427450838093037	25.78981879053342	24.50536707017406
37	24.73949602260425	25.010818408477913	25.931905053508764	24.31778051540907
38	24.2270723212481	24.881484822440154	26.13747730608284	24.753965550228905
39	24.38982076978487	24.88602060700643	26.068638340979444	24.65552028222926
40	24.39161259211766	24.6554003275961	26.60757951342802	24.345407566858217
41	23.78029379288753	24.949956426479506	26.844692337358012	24.42505744327495
42	24.021912412725506	24.769604635526843	26.766227012943705	24.442255938803946
43	23.671195054030566	24.14159714795864	26.892291835227915	25.294915962782877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	733.0
1	832.0
2	931.0
3	1422.5
4	1914.0
5	1914.0
6	2936.0
7	3958.0
8	4005.5
9	4053.0
10	6020.0
11	7987.0
12	7987.0
13	14223.5
14	20460.0
15	29689.0
16	38918.0
17	41659.0
18	44400.0
19	44400.0
20	55740.0
21	67080.0
22	75121.0
23	83162.0
24	105823.5
25	128485.0
26	128485.0
27	158621.5
28	188758.0
29	219173.5
30	249589.0
31	293283.0
32	336977.0
33	336977.0
34	392258.5
35	447540.0
36	511189.0
37	574838.0
38	633782.0
39	692726.0
40	692726.0
41	752590.5
42	812455.0
43	858138.5
44	903822.0
45	960013.5
46	1016205.0
47	1016205.0
48	1072543.0
49	1128881.0
50	1131964.5
51	1135048.0
52	1121090.0
53	1107132.0
54	1107132.0
55	1061677.0
56	1016222.0
57	959217.5
58	902213.0
59	833074.0
60	763935.0
61	763935.0
62	667477.5
63	571020.0
64	498374.5
65	425729.0
66	350855.5
67	275982.0
68	275982.0
69	219492.5
70	163003.0
71	131610.0
72	100217.0
73	77956.5
74	55696.0
75	55696.0
76	42362.5
77	29029.0
78	23433.5
79	17838.0
80	13536.0
81	9234.0
82	9234.0
83	7281.0
84	5328.0
85	4241.5
86	3155.0
87	2508.0
88	1861.0
89	1861.0
90	1395.5
91	930.0
92	681.5
93	433.0
94	411.0
95	389.0
96	389.0
97	215.5
98	42.0
99	55.0
100	68.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.3338376E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.44099168404882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16700317030734	34.15663165976806
2	12.19545043448664	11.083467235534739
3	4.604458453532604	6.276934748895699
4	2.2250366201234684	4.044314822069384
5	1.356925888395083	3.083002900521576
6	0.8611471595462421	2.347882854940983
7	0.6138244658377212	1.9524954713318314
8	0.4499870529937216	1.635828634641387
9	0.34072961831377846	1.3934782577074982
>10	1.9515853574625126	16.332711834386593
>50	0.14145077259783664	4.415541949154936
>100	0.08171040090067822	6.889536336435498
>500	0.0067897445216150865	2.153086592471366
>1k	0.0036033434044147063	3.067558247441608
>5k	2.479313134812332E-4	0.8159239775825902
>10k+	4.958626269624664E-5	0.3516044771162888
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16533	0.12395062187480693	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16271	0.12198636475684896	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14009	0.1050277784941735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.497164572358734E-5
2	0.0	0.0	0.0	0.0	7.497164572358734E-5
3	0.0	7.497164572358734E-6	0.0	0.0	7.497164572358734E-5
4	0.0	7.497164572358734E-6	0.0	0.0	8.246881029594607E-5
5	0.0	7.497164572358734E-6	0.0	7.497164572358734E-6	8.996597486830481E-5
6	0.0	7.497164572358734E-6	0.0	7.497164572358734E-6	1.1995463315773975E-4
7	0.0	7.497164572358734E-6	0.0	1.4994329144717469E-5	1.1995463315773975E-4
8	0.0	7.497164572358734E-6	0.0	1.4994329144717469E-5	1.2745179773009847E-4
9	0.0	7.497164572358734E-6	0.0	2.9988658289434937E-5	1.7243478516425087E-4
10	0.0	7.497164572358734E-6	0.0	5.248015200651114E-5	1.7243478516425087E-4
11	0.0	7.497164572358734E-6	0.0	5.9977316578869875E-5	1.7993194973660962E-4
12	0.0	7.497164572358734E-6	0.0	5.9977316578869875E-5	2.7739508917727315E-4
13	0.0	7.497164572358734E-6	0.0	5.9977316578869875E-5	3.1488091203906685E-4
14	0.0	7.497164572358734E-6	0.0	5.9977316578869875E-5	3.4486957032850175E-4
15	0.0	7.497164572358734E-6	0.0	5.9977316578869875E-5	4.198412160520891E-4
16	0.0	7.497164572358734E-6	0.0	1.1995463315773975E-4	4.4982987434152405E-4
17	0.0	7.497164572358734E-6	0.0	1.1995463315773975E-4	4.4982987434152405E-4
18	0.0	7.497164572358734E-6	0.0	1.349489623024572E-4	4.873156972033177E-4
19	0.0	1.4994329144717469E-5	0.0	1.4994329144717467E-4	5.098071909203939E-4
20	0.0	1.4994329144717469E-5	0.0	1.7993194973660962E-4	5.922760012163399E-4
21	0.0	1.4994329144717469E-5	0.0	2.1741777259840327E-4	6.372589886504923E-4
22	0.0	1.4994329144717469E-5	0.0	3.6736106404557795E-4	6.972363052293622E-4
23	0.0	1.4994329144717469E-5	0.0	5.62287342926905E-4	7.497164572358734E-4
24	0.0	1.4994329144717469E-5	0.0	8.621739258212544E-4	7.497164572358734E-4
25	0.0	1.4994329144717469E-5	0.0	0.0011170775212814513	7.497164572358734E-4
26	0.0	1.4994329144717469E-5	0.0	0.002226657877990544	8.096937738147433E-4
27	0.0	1.4994329144717469E-5	0.0	0.008374332827324705	8.321852675318194E-4
28	0.0	1.4994329144717469E-5	0.0	0.016606219527774597	8.546767612488957E-4
29	0.0	1.4994329144717469E-5	0.0	0.028496722539535547	8.621739258212544E-4
30	0.0	1.4994329144717469E-5	0.0	0.0471646623247088	8.621739258212544E-4
31	0.0	1.4994329144717469E-5	0.0	0.11673834955619784	8.696710903936131E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6110	0.0	25.19149	1
GTATCAA	10765	0.0	14.590339	1
AAGACGG	2925	0.0	13.9777775	5
CAAGACG	2960	0.0	13.6875	4
CGAACGA	2200	0.0	13.286365	16
ACGAACG	2295	0.0	13.139434	15
ACGGACC	2690	0.0	12.929368	8
TACCGTC	2205	0.0	12.417233	7
GCGCAAG	2995	0.0	12.415692	1
ATACCGT	2420	0.0	12.384298	6
CGGACCA	2840	0.0	12.3767605	9
TAACGAA	2355	0.0	12.333333	13
TATACTG	2280	0.0	12.333333	5
TCTAGCG	1260	0.0	12.186507	28
ACCGTCG	2210	0.0	12.138009	8
ATCGCTC	3045	0.0	12.090312	16
TAACGCC	2115	0.0	12.070922	4
CGTCGTA	2190	0.0	11.995434	10
TATACCG	620	0.0	11.935483	5
GCGGGTA	1620	0.0	11.876543	23
>>END_MODULE