FastQCFastQC Report
Thu 9 Feb 2017
SRR2727481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629811
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1112690.7119024024026906No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC790440.505725884977112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT780670.4994750096466298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG737990.4721682175171536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT717800.45925059490482645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT575920.3684753449673832No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA507000.32438012206289635No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG407730.2608668780447825No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG278870.1784218631946349No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT264120.1689847689137124No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG259180.16582414208335597No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC208930.13367404122800974No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA204240.13067336514817743No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166520.10653999590909961No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT165650.1059833672972757No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165090.10562507761610168No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161540.10335377695865931No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT160990.10300188530750629No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC159420.10199739459421486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG36700.015.5258864
AAGACGG36150.015.3015215
CGAACGA21150.014.95744616
ACGAACG22850.014.08752715
CTAGCGG21100.014.02843629
GCGCAAG41450.013.7466831
TAACGAA23300.013.736052513
TCTAGCG21150.013.7328628
CGATAAC24200.013.60743810
CGCCGGT22300.013.6053817
AACGAAC23850.013.49685514
ACGGACC39450.013.4588088
CGCAAGA41700.013.3980822
CCGATAA24650.013.3590279
ATAACGA24750.013.30505112
CGGTCCA24050.013.23076910
TATACTG23600.013.1694925
CGCTTCG22150.012.94582432
TAGCGGC23500.012.75319230
CCGGTCC26350.012.707789
Produced by FastQC (version 0.11.5)