##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15629811
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486909918488458	31.0	31.0	34.0	30.0	34.0
2	31.65127690923454	31.0	31.0	34.0	30.0	34.0
3	31.757103460816	31.0	31.0	34.0	30.0	34.0
4	35.4683365013179	37.0	35.0	37.0	33.0	37.0
5	35.361903032608645	37.0	35.0	37.0	33.0	37.0
6	35.37547626135722	37.0	35.0	37.0	33.0	37.0
7	35.239786520771105	37.0	35.0	37.0	32.0	37.0
8	35.202996568544556	37.0	35.0	37.0	32.0	37.0
9	36.79324151776371	39.0	37.0	39.0	32.0	39.0
10	36.650020336138425	39.0	35.0	39.0	32.0	39.0
11	36.68994551501614	39.0	35.0	39.0	32.0	39.0
12	36.530159385804474	39.0	35.0	39.0	32.0	39.0
13	36.60065582366927	39.0	35.0	39.0	32.0	39.0
14	37.60540098661462	39.0	36.0	41.0	32.0	41.0
15	37.569598698282405	39.0	36.0	41.0	32.0	41.0
16	37.46118068862125	39.0	36.0	41.0	32.0	41.0
17	37.52270376142104	39.0	36.0	41.0	32.0	41.0
18	37.4077262994415	39.0	36.0	41.0	32.0	41.0
19	37.444177667919334	39.0	36.0	41.0	32.0	41.0
20	37.393324077943106	39.0	36.0	41.0	32.0	41.0
21	37.23232193914565	39.0	36.0	41.0	31.0	41.0
22	37.090235640085474	39.0	36.0	40.0	31.0	41.0
23	37.2028562597462	39.0	36.0	41.0	31.0	41.0
24	37.18976742585051	39.0	36.0	41.0	31.0	41.0
25	37.156141043548125	39.0	36.0	41.0	31.0	41.0
26	36.947598790541996	39.0	36.0	41.0	31.0	41.0
27	36.886942714790344	39.0	36.0	41.0	30.0	41.0
28	36.619618688927204	39.0	35.0	41.0	30.0	41.0
29	36.47535981081281	39.0	35.0	40.0	30.0	41.0
30	36.3726007947249	39.0	35.0	40.0	30.0	41.0
31	36.21992441239372	38.0	35.0	40.0	29.0	41.0
32	36.069156178536005	38.0	35.0	40.0	29.0	41.0
33	35.861945355577234	38.0	35.0	40.0	28.0	41.0
34	35.78674265478962	38.0	35.0	40.0	27.0	41.0
35	35.56738050127414	38.0	34.0	40.0	27.0	41.0
36	35.4583276790743	38.0	34.0	40.0	26.0	41.0
37	35.34927261756396	38.0	34.0	40.0	26.0	41.0
38	35.2498231744453	38.0	34.0	40.0	25.0	41.0
39	35.16688192838672	38.0	34.0	40.0	25.0	41.0
40	34.9571526488708	38.0	33.0	40.0	24.0	41.0
41	34.78457167524291	38.0	33.0	40.0	24.0	41.0
42	34.744806191194506	38.0	33.0	40.0	24.0	41.0
43	34.452557103857494	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	14.0
11	21.0
12	61.0
13	87.0
14	117.0
15	302.0
16	620.0
17	991.0
18	2040.0
19	4294.0
20	8526.0
21	15770.0
22	27553.0
23	45359.0
24	70804.0
25	103890.0
26	148958.0
27	204773.0
28	275230.0
29	356217.0
30	450571.0
31	556075.0
32	678098.0
33	827568.0
34	1011302.0
35	1233930.0
36	1548696.0
37	2044769.0
38	2860129.0
39	3153045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78849565103506	20.048297449022257	12.2022460796231	24.96096082031958
2	17.798942034551793	23.983213872515798	33.9497963219133	24.268047771019113
3	20.43867964878142	26.21114868247607	29.89807746235703	23.452094206385475
4	14.554814514391762	16.670847779285367	34.02937501931405	34.744962687008815
5	12.90135242198386	39.64212363156535	33.86997449937175	13.586549447079047
6	33.64223662077552	35.34780426967415	15.996066747064312	15.01389236248602
7	26.952072549053856	32.248105879207365	22.150261445899762	18.649560125839013
8	28.23068685859349	33.63467414929074	20.577145814495136	17.557493177620636
9	27.978732436367913	13.441320563633175	19.520677505313405	39.05926949468551
10	16.96753722741753	28.168555589059906	33.048320289989434	21.815586893533133
11	35.21473804129813	22.96564558586153	20.630268657759203	21.189347715081137
12	21.453912654478035	27.301904034540147	29.878774605783782	21.365408705198035
13	30.243449520918713	20.173686041373116	25.672927203022482	23.909937234685692
14	22.52846819452903	21.830276770461268	26.3848999837554	29.256355051254303
15	25.916788117271537	27.487907563309626	23.22536721653256	23.369937102886272
16	23.14991524849533	26.729062814643118	26.700220495308614	23.42080144155294
17	23.69095186115814	27.523237485085396	26.26973544337804	22.51607521037842
18	23.671335501113866	24.77441345899832	28.344814918107453	23.209436121780357
19	23.38291870579881	26.918828385064923	28.243713247716173	21.454539661420093
20	23.468313212488624	25.426820580236065	28.09284769982183	23.01201850745348
21	23.361997147630255	26.504159263346178	28.153277093369844	21.980566495653722
22	23.818874073397307	26.217246005086047	26.436327349063916	23.52755257245273
23	22.422337672541275	26.085037112732838	27.58947628989244	23.90314892483345
24	22.505844760374902	27.69598429565143	27.307841406399604	22.490329537574063
25	23.317665197614993	25.794848063101977	26.473020051234148	24.414466688048883
26	23.34339807435931	26.505445267380388	27.58889406916053	22.562262589099767
27	23.730370124117304	26.07615664706374	26.510103033235655	23.6833701955833
28	22.717773106789327	26.712907788840184	27.97314695615961	22.596172148210876
29	23.18842499119151	25.941964365404036	27.847975896829464	23.02163474657499
30	22.201087396386303	27.615484281927653	28.0502944021524	22.13313391953364
31	23.915650675494412	26.10161440851716	26.48712130940035	23.49561360658808
32	21.557957418678956	27.20810251640279	27.50914902297923	23.72479104193902
33	22.415645333139345	26.717994222706853	27.710405455318686	23.155954988835116
34	22.538090831680563	26.401502871659805	27.699087340211598	23.361318956448034
35	22.27880426705096	27.106258674529077	28.51431152942284	22.10062552899712
36	22.898338310040987	26.49341697094098	27.423824894619646	23.18441982439839
37	22.565032936098845	26.378361197073975	27.999551626056128	23.05705424077105
38	22.63845033058941	26.081422225770996	28.33144943339366	22.94867801024593
39	22.96762897516803	25.034909251301883	27.78954908667802	24.20791268685207
40	22.381479852827397	26.043098025945422	28.63255992027031	22.942862200956878
41	21.69424185615552	26.04860033176345	28.60350006791509	23.65365774416594
42	22.313424007494394	26.369896603356242	28.629469671770185	22.68720971737918
43	21.96369489048844	25.1001307693356	28.82974080748641	24.10643353268955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9022.0
1	8536.5
2	8051.0
3	12724.0
4	17397.0
5	17397.0
6	27225.0
7	37053.0
8	35893.5
9	34734.0
10	46569.0
11	58404.0
12	58404.0
13	102331.0
14	146258.0
15	199285.5
16	252313.0
17	223455.0
18	194597.0
19	194597.0
20	212241.5
21	229886.0
22	198924.5
23	167963.0
24	190282.5
25	212602.0
26	212602.0
27	240161.5
28	267721.0
29	295353.5
30	322986.0
31	355871.0
32	388756.0
33	388756.0
34	431822.5
35	474889.0
36	535213.5
37	595538.0
38	659423.5
39	723309.0
40	723309.0
41	783009.5
42	842710.0
43	893364.5
44	944019.0
45	1032813.0
46	1121607.0
47	1121607.0
48	1344180.5
49	1566754.0
50	1597434.0
51	1628114.0
52	1384143.0
53	1140172.0
54	1140172.0
55	1078556.0
56	1016940.0
57	950440.0
58	883940.0
59	806718.5
60	729497.0
61	729497.0
62	627675.5
63	525854.0
64	457295.5
65	388737.0
66	325964.0
67	263191.0
68	263191.0
69	212391.0
70	161591.0
71	136189.0
72	110787.0
73	86123.5
74	61460.0
75	61460.0
76	48108.0
77	34756.0
78	29805.0
79	24854.0
80	19984.5
81	15115.0
82	15115.0
83	12812.5
84	10510.0
85	8869.0
86	7228.0
87	5950.0
88	4672.0
89	4672.0
90	3745.0
91	2818.0
92	2134.0
93	1450.0
94	1304.0
95	1158.0
96	1158.0
97	693.0
98	228.0
99	199.0
100	170.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.5629811E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.74866014424751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3651893051574	31.881443351521405
2	11.757048272716435	9.816820252743012
3	4.311468070640459	5.3999404561182915
4	2.1482603628276498	3.587479671561975
5	1.2486647247566807	2.6065039613988508
6	0.8216160949142471	2.058082266937122
7	0.5681734291003301	1.6604335576150973
8	0.40799071978913887	1.362645272198694
9	0.31443441540427175	1.1814493991731274
>10	1.8234625759293026	14.013886812457446
>50	0.13916781618768076	4.005519442717099
>100	0.0782115249191948	6.230862806035879
>500	0.008287208045277758	2.4085605548513933
>1k	0.006808598931921892	5.67680875992969
>5k	7.701776457221729E-4	2.288327672161936
>10k+	4.467030345188604E-4	5.821235762578874
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	111269	0.7119024024026906	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	79044	0.505725884977112	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	78067	0.4994750096466298	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	73799	0.4721682175171536	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	71780	0.45925059490482645	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57592	0.3684753449673832	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	50700	0.32438012206289635	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	40773	0.2608668780447825	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	27887	0.1784218631946349	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	26412	0.1689847689137124	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	25918	0.16582414208335597	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	20893	0.13367404122800974	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	20424	0.13067336514817743	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	16652	0.10653999590909961	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	16565	0.1059833672972757	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16509	0.10562507761610168	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16154	0.10335377695865931	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	16099	0.10300188530750629	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15942	0.10199739459421486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9194090062893276E-5
2	0.0	6.3980300209644246E-6	0.0	0.0	1.9194090062893276E-5
3	0.0	6.3980300209644246E-6	0.0	0.0	1.9194090062893276E-5
4	0.0	6.3980300209644246E-6	0.0	0.0	1.9194090062893276E-5
5	0.0	6.3980300209644246E-6	0.0	0.0	1.9194090062893276E-5
6	0.0	6.3980300209644246E-6	0.0	0.0	3.1990150104822124E-5
7	0.0	6.3980300209644246E-6	0.0	0.0	3.1990150104822124E-5
8	0.0	6.3980300209644246E-6	0.0	6.3980300209644246E-6	4.4786210146750974E-5
9	0.0	6.3980300209644246E-6	0.0	5.1184240167715396E-5	5.7582270188679825E-5
10	0.0	6.3980300209644246E-6	0.0	8.957242029350195E-5	6.398030020964425E-5
11	0.0	6.3980300209644246E-6	0.0	1.7274681056603946E-4	6.398030020964425E-5
12	0.0	6.3980300209644246E-6	0.0	3.1990150104822124E-4	8.957242029350195E-5
13	0.0	6.3980300209644246E-6	0.0	3.646877111949722E-4	8.957242029350195E-5
14	0.0	6.3980300209644246E-6	0.0	4.926483116142607E-4	1.0236848033543079E-4
15	0.0	1.9194090062893276E-5	0.0	7.67763602515731E-4	1.0876651035639523E-4
16	0.0	1.9194090062893276E-5	0.0	0.001612303565283035	1.4075666046121734E-4
17	0.0	1.9194090062893276E-5	0.0	0.0025336198883019125	1.4715469048218177E-4
18	0.0	1.9194090062893276E-5	0.0	0.003083850470104853	1.7274681056603946E-4
19	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.0045553973749266705	1.7274681056603946E-4
20	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.005905381709350164	1.8554287060796833E-4
21	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.009737801691907855	1.9833893064989718E-4
22	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.01642374306381568	1.9833893064989718E-4
23	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.026558222617023328	2.2393105073375487E-4
24	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.04086421774389978	2.2393105073375487E-4
25	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.046315339321761476	2.2393105073375487E-4
26	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.05943769889475951	2.303290807547193E-4
27	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.11106980116394241	2.303290807547193E-4
28	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.1804948249214274	2.303290807547193E-4
29	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.25444325590373423	2.303290807547193E-4
30	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.3604714094111567	2.303290807547193E-4
31	6.3980300209644246E-6	1.9194090062893276E-5	0.0	0.5894760979515363	2.303290807547193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	3670	0.0	15.525886	4
AAGACGG	3615	0.0	15.301521	5
CGAACGA	2115	0.0	14.957446	16
ACGAACG	2285	0.0	14.087527	15
CTAGCGG	2110	0.0	14.028436	29
GCGCAAG	4145	0.0	13.746683	1
TAACGAA	2330	0.0	13.7360525	13
TCTAGCG	2115	0.0	13.73286	28
CGATAAC	2420	0.0	13.607438	10
CGCCGGT	2230	0.0	13.605381	7
AACGAAC	2385	0.0	13.496855	14
ACGGACC	3945	0.0	13.458808	8
CGCAAGA	4170	0.0	13.398082	2
CCGATAA	2465	0.0	13.359027	9
ATAACGA	2475	0.0	13.305051	12
CGGTCCA	2405	0.0	13.230769	10
TATACTG	2360	0.0	13.169492	5
CGCTTCG	2215	0.0	12.945824	32
TAGCGGC	2350	0.0	12.753192	30
CCGGTCC	2635	0.0	12.70778	9
>>END_MODULE