FastQCFastQC Report
Thu 9 Feb 2017
SRR2727480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15573201
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT489880.3145660291676708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT445500.2860683555037914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT394670.2534289514403622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284300.18255720195225117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG39600.016.2108575
CAAGACG40450.015.778744
GGTATCA260300.014.4560131
TCTAGCG19600.014.25255228
CTAGCGG20100.013.71393129
ACGGACC45300.013.6810158
TCGTTAC40350.013.617123
CGCTTCG21550.013.47795932
TAGAGTG26800.013.460825
ACGAACG30200.013.41556415
CGAACGA30050.013.17470816
TACGGAT70850.013.10797427
GACGGAC47150.013.1049847
ACGGATG72200.013.04224428
GCGCAAG49800.012.8162651
CGCAAGA49550.012.6195762
CGAACGT9250.012.64
CGAGCCG39150.012.5223515
CGGACCA49450.012.495459
AGACGGA52800.012.4384476
Produced by FastQC (version 0.11.5)