FastQCFastQC Report
Thu 9 Feb 2017
SRR2727479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139060
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66430.21162386192044752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60920.19407083649245316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48510.15453670844138054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.024.041851
TAAGGCG4050.016.4444455
GTATCAA35600.015.1221912
TAGACCG1508.1150574E-814.85
TAATACT7700.014.4155844
CGCTACG3800.014.11842228
TTTCGCG800.00630182613.87521
CCGTCGT1753.5723133E-813.7428579
ACCGTCG2408.185452E-1113.1041668
CGTACGC1000.001833708612.9500013
TATACAC9300.012.7311823
GGGTAAG10950.012.6712321
ATTATAC5950.012.4369753
ATCTCCG6100.012.43442610
TAGACTG4350.012.3333345
GATAACG2401.1295924E-912.33333311
CTGTGCG5100.012.3333339
TACGGAT6650.012.24060227
TCTTATA12900.012.18992237
GACGCTA5050.012.08910926
Produced by FastQC (version 0.11.5)