##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3139060
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519589940937735	31.0	31.0	34.0	30.0	34.0
2	31.732964326900408	31.0	31.0	34.0	30.0	34.0
3	31.83031576331768	31.0	31.0	34.0	30.0	34.0
4	35.56055538919294	37.0	35.0	37.0	33.0	37.0
5	35.42986945136442	37.0	35.0	37.0	33.0	37.0
6	35.40698234503323	37.0	35.0	37.0	33.0	37.0
7	35.29664740399993	37.0	35.0	37.0	32.0	37.0
8	35.21989831350786	37.0	35.0	37.0	32.0	37.0
9	36.833172032391865	39.0	37.0	39.0	33.0	39.0
10	36.67534867125828	39.0	35.0	39.0	32.0	39.0
11	36.709260096971704	39.0	35.0	39.0	32.0	39.0
12	36.54335724707396	39.0	35.0	39.0	32.0	39.0
13	36.62759934502686	39.0	35.0	39.0	32.0	39.0
14	37.6711658904258	39.0	37.0	41.0	32.0	41.0
15	37.63696361331099	39.0	36.0	41.0	32.0	41.0
16	37.54930743598402	39.0	36.0	41.0	32.0	41.0
17	37.53311787605207	39.0	36.0	41.0	32.0	41.0
18	37.405560900396935	39.0	36.0	41.0	32.0	41.0
19	37.466025179512336	39.0	36.0	41.0	32.0	41.0
20	37.356310169286346	39.0	36.0	41.0	31.0	41.0
21	37.19398004498162	39.0	36.0	41.0	31.0	41.0
22	37.030618720253834	39.0	36.0	40.0	31.0	41.0
23	37.19936987505814	39.0	36.0	41.0	31.0	41.0
24	37.19575350582659	39.0	36.0	41.0	31.0	41.0
25	37.1491765050684	39.0	36.0	41.0	31.0	41.0
26	36.976430842354084	39.0	36.0	41.0	31.0	41.0
27	36.93517294986397	39.0	36.0	41.0	30.0	41.0
28	36.691455722413714	39.0	36.0	41.0	30.0	41.0
29	36.577813421852404	39.0	35.0	40.0	30.0	41.0
30	36.53372187852415	39.0	35.0	40.0	30.0	41.0
31	36.386896714303006	39.0	35.0	40.0	30.0	41.0
32	36.34853491172517	39.0	35.0	40.0	30.0	41.0
33	36.11230177186801	38.0	35.0	40.0	29.0	41.0
34	36.07756430268934	38.0	35.0	40.0	29.0	41.0
35	35.877714347607245	38.0	35.0	40.0	27.0	41.0
36	35.76431320204138	38.0	34.0	40.0	27.0	41.0
37	35.709094760851976	38.0	34.0	40.0	27.0	41.0
38	35.65985932094321	38.0	34.0	40.0	27.0	41.0
39	35.615132874172524	38.0	34.0	40.0	26.0	41.0
40	35.45661758615636	38.0	34.0	40.0	26.0	41.0
41	35.332048129057746	38.0	34.0	40.0	25.0	41.0
42	35.36015367657834	38.0	34.0	40.0	26.0	41.0
43	35.12260804189789	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	3.0
14	4.0
15	14.0
16	51.0
17	94.0
18	215.0
19	522.0
20	1159.0
21	2271.0
22	4488.0
23	7773.0
24	12398.0
25	18652.0
26	27158.0
27	37748.0
28	51682.0
29	67493.0
30	86617.0
31	108554.0
32	133093.0
33	162255.0
34	200314.0
35	244009.0
36	306978.0
37	398702.0
38	583658.0
39	683149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64532057367492	20.728466483597	12.819124196415487	25.80708874631259
2	18.55262403394647	22.479309092530887	34.853459315846145	24.1146075576765
3	19.25907755824992	24.434767095882208	31.37620816422751	24.92994718164036
4	14.583028040241347	16.78601237313081	35.548030302064944	33.082929284562894
5	13.339980758571038	38.191656100870965	35.12385873478048	13.344504405777528
6	33.89062330761438	36.755398112810845	15.51327467458411	13.840703904990667
7	27.52566054806216	32.24086191407619	21.796939211101414	18.43653832676024
8	26.78699992991532	34.85489286601722	20.125865705019976	18.232241499047486
9	26.765719674042547	14.400744171822138	19.87690582531076	38.95663032882455
10	15.96379170834581	28.01765496677349	34.63995591036807	21.378597414512626
11	35.605690875612446	22.54397813358139	21.726981962753182	20.123349028052985
12	20.741846285193656	26.153816747688797	30.393844016998717	22.710492950118823
13	29.885698266359995	21.173153746663015	24.936955649143375	24.00419233783362
14	22.006173822736745	21.76874605773703	25.68784922875001	30.537230890776218
15	24.523296783113416	29.029072397469307	22.500748631756004	23.946882187661274
16	23.986416315712347	27.32461310073716	24.9619949921314	23.726975591419087
17	22.936930163807	27.460354373602286	26.090804253502643	23.511911209088073
18	23.06464355571413	26.75539811281084	26.863105515663925	23.316852815811103
19	23.70461220875039	26.571425840856815	27.02490554497206	22.69905640542073
20	23.31780851592515	26.340656119984963	26.87148381999707	23.47005154409282
21	23.628570336342726	26.410358514969452	26.99725395500564	22.963817193682186
22	24.056246137378707	26.310551566392487	26.49605295852899	23.13714933769982
23	23.709964129389054	26.394971743133294	26.480029053283467	23.415035074194186
24	23.514236746032253	26.461615897752832	26.523895688518216	23.5002516676967
25	23.847202665766183	26.258625193529273	26.38926939911948	23.50490274158506
26	23.680337425853597	26.459226647467716	26.587895739488893	23.272540187189797
27	23.84057647830879	26.726058119309602	26.01262798417361	23.420737418208
28	23.497320854013623	26.854631641319376	26.219218492160074	23.42882901250693
29	23.547495110001083	26.738259224098933	26.422081769701755	23.292163896198225
30	23.676642052079284	26.611246678942102	26.214694844953584	23.497416424025026
31	23.462151089816697	26.29267997425981	26.705606136868997	23.539562799054494
32	23.283307741808056	26.722776882251374	26.20182475008442	23.79209062585615
33	23.110485304517915	26.53236956286277	26.561359132988855	23.79578599963046
34	23.844685988799196	26.495256541767283	26.56489522341083	23.095162246022692
35	23.446222754582582	26.430842354080518	26.78833791007499	23.334596981261907
36	23.424241651959505	26.332118532299475	26.81000044599339	23.43363936974763
37	23.435678196657598	26.141870496263213	26.9943868546635	23.428064452415693
38	23.2740374507018	25.901671200932764	27.067593483399488	23.756697864965943
39	23.014246303033392	25.922696603441796	27.386415041445527	23.676642052079284
40	23.35845762744261	25.596516154517595	27.83275885137589	23.212267366663905
41	22.745344147611068	25.635253865806963	27.788032086038495	23.831369900543475
42	22.581887571438582	25.82926098895848	27.664077781246615	23.924773658356322
43	22.5060686957242	25.07228278529241	27.90191968296242	24.519728836020974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1121.0
1	954.5
2	788.0
3	1032.5
4	1277.0
5	1277.0
6	1815.5
7	2354.0
8	2541.5
9	2729.0
10	3850.0
11	4971.0
12	4971.0
13	7934.0
14	10897.0
15	14189.5
16	17482.0
17	18007.5
18	18533.0
19	18533.0
20	22876.5
21	27220.0
22	28944.5
23	30669.0
24	37937.0
25	45205.0
26	45205.0
27	52928.5
28	60652.0
29	68149.0
30	75646.0
31	86327.5
32	97009.0
33	97009.0
34	108822.0
35	120635.0
36	134720.0
37	148805.0
38	159514.0
39	170223.0
40	170223.0
41	182994.5
42	195766.0
43	207816.5
44	219867.0
45	230786.0
46	241705.0
47	241705.0
48	252051.0
49	262397.0
50	261203.0
51	260009.0
52	255216.0
53	250423.0
54	250423.0
55	236884.5
56	223346.0
57	205980.0
58	188614.0
59	170671.5
60	152729.0
61	152729.0
62	132865.0
63	113001.0
64	96232.5
65	79464.0
66	64574.5
67	49685.0
68	49685.0
69	39659.5
70	29634.0
71	23034.5
72	16435.0
73	12769.5
74	9104.0
75	9104.0
76	6985.5
77	4867.0
78	3850.5
79	2834.0
80	2143.5
81	1453.0
82	1453.0
83	1083.5
84	714.0
85	547.5
86	381.0
87	287.5
88	194.0
89	194.0
90	163.0
91	132.0
92	84.0
93	36.0
94	35.0
95	34.0
96	34.0
97	23.0
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3139060.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.584793682126424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57419448993427	43.89519790270079
2	12.286308878046965	13.90436505958331
3	4.140211942359475	7.028191156760601
4	1.865106406527588	4.22146644834303
5	1.0499810162367251	2.970647958695226
6	0.689336752185735	2.340358673996215
7	0.45313448417989693	1.7948364908303371
8	0.3183564150161104	1.4411305648854413
9	0.24656793215909106	1.255679601286564
>10	1.25678501283919	12.54414043319761
>50	0.07932972434476915	3.082777996850811
>100	0.03803282235539406	3.835418971748792
>500	0.0020894166207827925	0.7742095917714285
>1k	4.5176575521182805E-4	0.5046469961329496
>5k	1.1294143880295701E-4	0.40693215321681203
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6643	0.21162386192044752	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6092	0.19407083649245316	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4851	0.15453670844138054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.371334093645869E-5	0.0
5	0.0	0.0	0.0	6.371334093645869E-5	0.0
6	0.0	0.0	0.0	6.371334093645869E-5	3.1856670468229345E-5
7	0.0	0.0	0.0	6.371334093645869E-5	3.1856670468229345E-5
8	0.0	0.0	0.0	6.371334093645869E-5	6.371334093645869E-5
9	0.0	0.0	0.0	1.2742668187291738E-4	9.557001140468803E-5
10	0.0	0.0	0.0	1.2742668187291738E-4	9.557001140468803E-5
11	0.0	0.0	0.0	1.2742668187291738E-4	9.557001140468803E-5
12	0.0	0.0	0.0	1.2742668187291738E-4	1.2742668187291738E-4
13	0.0	0.0	0.0	1.2742668187291738E-4	1.2742668187291738E-4
14	0.0	0.0	0.0	1.2742668187291738E-4	1.9114002280937607E-4
15	0.0	0.0	0.0	1.2742668187291738E-4	1.9114002280937607E-4
16	0.0	0.0	0.0	1.592833523411467E-4	2.5485336374583476E-4
17	0.0	0.0	0.0	1.9114002280937607E-4	2.5485336374583476E-4
18	0.0	0.0	0.0	1.9114002280937607E-4	2.5485336374583476E-4
19	0.0	0.0	0.0	1.9114002280937607E-4	2.5485336374583476E-4
20	0.0	0.0	0.0	2.229966932776054E-4	2.5485336374583476E-4
21	0.0	0.0	0.0	2.8671003421406406E-4	2.5485336374583476E-4
22	0.0	0.0	0.0	3.8228004561875214E-4	2.5485336374583476E-4
23	0.0	0.0	0.0	5.734200684281281E-4	2.5485336374583476E-4
24	0.0	0.0	0.0	0.0011468401368562562	2.5485336374583476E-4
25	0.0	0.0	0.0	0.001592833523411467	2.5485336374583476E-4
26	0.0	0.0	0.0	0.0032175237172911634	2.5485336374583476E-4
27	0.0	0.0	0.0	0.013857651653679763	2.5485336374583476E-4
28	0.0	0.0	0.0	0.027747159977827757	2.5485336374583476E-4
29	0.0	0.0	0.0	0.044025918587092955	3.185667046822934E-4
30	0.0	0.0	0.0	0.06963868164354935	3.185667046822934E-4
31	0.0	0.0	0.0	0.15479156180512638	3.185667046822934E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2270	0.0	24.04185	1
TAAGGCG	405	0.0	16.444445	5
GTATCAA	3560	0.0	15.122191	2
TAGACCG	150	8.1150574E-8	14.8	5
TAATACT	770	0.0	14.415584	4
CGCTACG	380	0.0	14.118422	28
TTTCGCG	80	0.006301826	13.875	21
CCGTCGT	175	3.5723133E-8	13.742857	9
ACCGTCG	240	8.185452E-11	13.104166	8
CGTACGC	100	0.0018337086	12.950001	3
TATACAC	930	0.0	12.731182	3
GGGTAAG	1095	0.0	12.671232	1
ATTATAC	595	0.0	12.436975	3
ATCTCCG	610	0.0	12.434426	10
TAGACTG	435	0.0	12.333334	5
GATAACG	240	1.1295924E-9	12.333333	11
CTGTGCG	510	0.0	12.333333	9
TACGGAT	665	0.0	12.240602	27
TCTTATA	1290	0.0	12.189922	37
GACGCTA	505	0.0	12.089109	26
>>END_MODULE