FastQCFastQC Report
Thu 9 Feb 2017
SRR2727478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10432421
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT437930.41977792115559753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT426980.4092817956637294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT345500.33117911940095207No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273270.2619430331655519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104350.025.5826551
GTATCAA173800.015.296032
TAAGGCG12050.014.2780085
ATTACGC1203.3053846E-513.8749993
CGCCTTA9000.013.36111233
TATACCG3900.012.8076935
ACCCGAC32650.012.74885111
CTTATAC81750.012.71804237
ACGAACG10600.012.56603815
CGCGCTA9600.012.52604124
TATACTG19450.012.1748085
GCACCCG35600.011.9002829
CCCGACT36150.011.77040112
CGATAAC11600.011.642241510
CGAACGA11150.011.61434916
TCTTATA116500.011.49699637
AAGACGG14400.011.4340295
GGGTAAG40350.011.4163571
TATTAAG23300.011.3540772
TAATACT27700.011.353794
Produced by FastQC (version 0.11.5)