##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727478.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10432421
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.427716442808432	31.0	31.0	34.0	30.0	34.0
2	31.63226953743527	31.0	31.0	34.0	30.0	34.0
3	31.723190810646926	31.0	31.0	34.0	30.0	34.0
4	35.46638829088665	37.0	35.0	37.0	33.0	37.0
5	35.33225068275139	37.0	35.0	37.0	33.0	37.0
6	35.31663973300157	37.0	35.0	37.0	33.0	37.0
7	35.204132482766944	37.0	35.0	37.0	32.0	37.0
8	35.11391948235218	37.0	35.0	37.0	32.0	37.0
9	36.68198139243039	39.0	35.0	39.0	32.0	39.0
10	36.54941408135274	38.0	35.0	39.0	32.0	39.0
11	36.57500123892623	39.0	35.0	39.0	32.0	39.0
12	36.41170012214806	38.0	35.0	39.0	32.0	39.0
13	36.499806612482374	38.0	35.0	39.0	32.0	39.0
14	37.51881332243014	39.0	36.0	41.0	32.0	41.0
15	37.48904784421564	39.0	36.0	41.0	32.0	41.0
16	37.42017964957511	39.0	36.0	41.0	32.0	41.0
17	37.41869169198598	39.0	36.0	41.0	32.0	41.0
18	37.28640130608226	39.0	36.0	41.0	32.0	41.0
19	37.35330955297912	39.0	36.0	41.0	31.0	41.0
20	37.25819040470088	39.0	36.0	41.0	31.0	41.0
21	37.08422829178385	39.0	36.0	40.0	31.0	41.0
22	36.887936558541874	39.0	36.0	40.0	30.0	41.0
23	37.05211436539994	39.0	36.0	40.0	31.0	41.0
24	37.029084907520506	39.0	36.0	41.0	31.0	41.0
25	36.974896718604434	39.0	36.0	41.0	30.0	41.0
26	36.79376436207856	39.0	36.0	41.0	30.0	41.0
27	36.65774023115056	39.0	35.0	40.0	30.0	41.0
28	36.405138270397636	39.0	35.0	40.0	30.0	41.0
29	36.323770867759265	39.0	35.0	40.0	29.0	41.0
30	36.29184452966383	39.0	35.0	40.0	29.0	41.0
31	36.13597054796772	38.0	35.0	40.0	29.0	41.0
32	36.06796035167676	38.0	35.0	40.0	29.0	41.0
33	35.84233496711837	38.0	34.0	40.0	27.0	41.0
34	35.818616119882435	38.0	34.0	40.0	27.0	41.0
35	35.61217391437712	38.0	34.0	40.0	27.0	41.0
36	35.500933196618504	38.0	34.0	40.0	26.0	41.0
37	35.45778558974949	38.0	34.0	40.0	26.0	41.0
38	35.39659528694251	38.0	34.0	40.0	26.0	41.0
39	35.35580686400597	38.0	34.0	40.0	25.0	41.0
40	35.17047701583362	38.0	34.0	40.0	25.0	41.0
41	35.03005131790598	38.0	33.0	40.0	24.0	41.0
42	35.066256720276144	38.0	33.0	40.0	25.0	41.0
43	34.82424252242121	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	10.0
12	14.0
13	20.0
14	30.0
15	77.0
16	174.0
17	394.0
18	901.0
19	2096.0
20	4418.0
21	8993.0
22	16885.0
23	29107.0
24	46578.0
25	69680.0
26	101066.0
27	140136.0
28	187877.0
29	245355.0
30	310156.0
31	384023.0
32	467042.0
33	564567.0
34	684304.0
35	834539.0
36	1036839.0
37	1326449.0
38	1863104.0
39	2107584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19122694530829	21.36762885623577	13.295063533191383	25.146080665264563
2	18.376578169151724	22.84872322541431	35.21271812170924	23.561980483724724
3	19.248293373129783	25.013129742367568	31.21666581515451	24.52191106934814
4	14.381829490968586	16.93469809165102	35.67935956572305	33.00411285165735
5	13.011457263850836	38.779819180993556	34.94623156024858	13.26249199490703
6	33.333585751571945	37.14528008407636	15.648390723495535	13.872743440856153
7	27.148952290173106	32.76397683720778	22.06937392576469	18.017696946854425
8	26.58205607308217	35.46924534583104	20.206757376835156	17.741941204251628
9	26.832985363608312	14.565238500248409	19.993134862943126	38.60864127320015
10	15.971920611716111	28.215454495174225	34.446213395720896	21.366411497388764
11	35.23535907916293	22.817896248627235	21.96140282298807	19.985341849221765
12	20.530555659132236	26.540148255136558	31.06437134774373	21.864924737987472
13	29.528812152040263	21.328721300645363	25.744426916820167	23.398039630494203
14	21.635351947548894	21.859135094337162	26.69735049994627	29.80816245816767
15	24.050831537569277	29.283183644525078	23.380306450439452	23.28567836746619
16	23.26216512926386	27.664364772088856	25.759734964683652	23.313735133963633
17	22.43838702445003	27.916492250456532	26.720499489044773	22.924621236048658
18	22.58077966753834	26.788230651351206	27.811291357969548	22.81969832314091
19	23.21435264163515	26.771849027181705	28.025191851440812	21.988606479742334
20	22.870127653015537	26.35383483852885	27.967698006052476	22.808339502403133
21	23.247758118657213	26.585171361470174	28.032956108653973	22.13411441121864
22	23.555050165249273	26.30534178020615	27.56566285045437	22.573945204090208
23	23.034892859481033	26.59722033840467	27.62420151564052	22.743685286473774
24	22.826762838654613	26.704319160432654	27.65162563895763	22.8172923619551
25	23.195210392678746	26.46707796780824	27.500979878016807	22.836731761496203
26	22.94107954424002	26.79552521893049	27.63696940527994	22.62642583154955
27	23.19948552689735	26.905605132308214	27.142980521970884	22.75192881882355
28	22.92445828250221	26.94355413762539	27.435175401759572	22.696812178112825
29	22.812336657042504	26.989257814652994	27.629233904574978	22.569171623729524
30	23.02174154973232	26.902480258417484	27.35658386485745	22.71919432699275
31	22.77810682678546	26.542860952409804	27.785448842603266	22.893583378201473
32	22.643967301549658	26.904349431450285	27.39860670883585	23.053076558164207
33	22.510700057062497	26.587337685087668	27.729028573520946	23.172933684328882
34	23.15684921074408	26.528166376721185	27.828104329762	22.486880082772736
35	22.813218523293873	26.3938830689444	28.04126673952288	22.751631668238847
36	22.697003888167476	26.25774017363755	27.984568490861328	23.060687447333652
37	22.629857441527715	25.87997551095762	28.362505692590435	23.127661354924232
38	22.45148082118235	25.48438181319561	28.600715020990812	23.463422344631223
39	22.22084403994049	25.51929221414665	28.90223659493803	23.35762715097483
40	22.381592920761154	25.358850069413418	29.292874587787438	22.96668242203799
41	21.624970848089816	25.419363348162427	29.514299700903557	23.4413661028442
42	21.496122520362242	25.711855378535812	29.36774694962943	23.42427515147251
43	21.169841592857498	25.148467455444905	29.58291272946136	24.098778222236238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15934.0
1	13606.5
2	11279.0
3	12333.0
4	13387.0
5	13387.0
6	18669.5
7	23952.0
8	23014.0
9	22076.0
10	29687.5
11	37299.0
12	37299.0
13	57722.5
14	78146.0
15	93324.0
16	108502.0
17	105682.0
18	102862.0
19	102862.0
20	117719.0
21	132576.0
22	127310.5
23	122045.0
24	143369.0
25	164693.0
26	164693.0
27	188025.0
28	211357.0
29	233903.5
30	256450.0
31	287764.0
32	319078.0
33	319078.0
34	355629.5
35	392181.0
36	433761.0
37	475341.0
38	513637.0
39	551933.0
40	551933.0
41	592227.0
42	632521.0
43	670313.5
44	708106.0
45	748233.0
46	788360.0
47	788360.0
48	820070.5
49	851781.0
50	849846.0
51	847911.0
52	822840.0
53	797769.0
54	797769.0
55	753849.0
56	709929.0
57	655541.0
58	601153.0
59	544366.5
60	487580.0
61	487580.0
62	421354.0
63	355128.0
64	302908.0
65	250688.0
66	202927.0
67	155166.0
68	155166.0
69	123158.0
70	91150.0
71	71754.5
72	52359.0
73	40615.0
74	28871.0
75	28871.0
76	22223.0
77	15575.0
78	12261.5
79	8948.0
80	6838.0
81	4728.0
82	4728.0
83	3593.0
84	2458.0
85	1953.0
86	1448.0
87	1131.0
88	814.0
89	814.0
90	622.0
91	430.0
92	321.0
93	212.0
94	191.0
95	170.0
96	170.0
97	103.0
98	36.0
99	37.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0432421E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.08117219338301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07770793707222	35.81827667463199
2	11.555914871256167	10.881320358113742
3	4.478312105372295	6.325325501062339
4	2.1818683670603285	4.108996811714509
5	1.267808488843387	2.98449548857341
6	0.8430675014726833	2.381556372448835
7	0.6137143742571469	2.022607449236853
8	0.44915671525412604	1.691745972215505
9	0.3303058410963336	1.3996067563023022
>10	1.9911535259965234	17.225844408917013
>50	0.13668843245071516	4.431875667069883
>100	0.06753046873229532	5.758631727833639
>500	0.004420678021766034	1.4072623540314546
>1k	0.002166726321040048	1.772678119294967
>5k	1.0220377381354605E-4	0.3619202514530629
>10k+	8.176301905083683E-5	1.4278560871004875
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43793	0.41977792115559753	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42698	0.4092817956637294	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34550	0.33117911940095207	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27327	0.2619430331655519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.585502732299626E-6	7.668402185839701E-5
2	0.0	0.0	0.0	9.585502732299626E-6	7.668402185839701E-5
3	0.0	0.0	0.0	9.585502732299626E-6	7.668402185839701E-5
4	0.0	0.0	0.0	1.9171005464599252E-5	7.668402185839701E-5
5	0.0	0.0	0.0	1.9171005464599252E-5	8.626952459069663E-5
6	0.0	0.0	0.0	1.9171005464599252E-5	1.2461153551989514E-4
7	0.0	0.0	0.0	1.9171005464599252E-5	1.3419703825219478E-4
8	0.0	0.0	0.0	1.9171005464599252E-5	1.5336804371679402E-4
9	0.0	0.0	0.0	3.8342010929198505E-5	1.821245519136929E-4
10	0.0	0.0	0.0	7.668402185839701E-5	1.9171005464599253E-4
11	0.0	0.0	0.0	9.585502732299627E-5	2.0129555737829216E-4
12	0.0	0.0	0.0	1.4378254098449438E-4	2.6839407650438955E-4
13	0.0	0.0	0.0	1.5336804371679402E-4	3.1632159016588767E-4
14	0.0	0.0	0.0	1.9171005464599253E-4	3.450780983627865E-4
15	0.0	0.0	0.0	2.8756508196898877E-4	4.121766174888839E-4
16	0.0	0.0	0.0	5.080316448118801E-4	4.792751366149813E-4
17	0.0	0.0	0.0	8.147677322454682E-4	4.88860639347281E-4
18	0.0	0.0	0.0	9.393792677653633E-4	5.751301639379775E-4
19	0.0	0.0	0.0	0.0011790168360728541	5.847156666702772E-4
20	0.0	0.0	0.0	0.0014569964153095432	7.093272021901723E-4
21	0.0	0.0	0.0	0.0019171005464599252	7.18912704922472E-4
22	0.0	0.0	0.0	0.0032974129399110713	7.18912704922472E-4
23	9.585502732299626E-6	0.0	0.0	0.005521249573804584	7.860112240485694E-4
24	9.585502732299626E-6	0.0	0.0	0.010409855967277394	7.95596726780869E-4
25	9.585502732299626E-6	0.0	0.0	0.01411944552467735	7.95596726780869E-4
26	9.585502732299626E-6	0.0	0.0	0.021739920196855552	8.435242404423671E-4
27	9.585502732299626E-6	0.0	0.0	0.07993350728464658	8.914517541038652E-4
28	9.585502732299626E-6	0.0	0.0	0.14711829593533465	9.010372568361649E-4
29	9.585502732299626E-6	0.0	0.0	0.22406112636750378	9.202082623007642E-4
30	9.585502732299626E-6	0.0	0.0	0.32932911737361825	9.202082623007642E-4
31	9.585502732299626E-6	0.0	0.0	0.585252454823286	9.297937650330637E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10435	0.0	25.582655	1
GTATCAA	17380	0.0	15.29603	2
TAAGGCG	1205	0.0	14.278008	5
ATTACGC	120	3.3053846E-5	13.874999	3
CGCCTTA	900	0.0	13.361112	33
TATACCG	390	0.0	12.807693	5
ACCCGAC	3265	0.0	12.748851	11
CTTATAC	8175	0.0	12.718042	37
ACGAACG	1060	0.0	12.566038	15
CGCGCTA	960	0.0	12.526041	24
TATACTG	1945	0.0	12.174808	5
GCACCCG	3560	0.0	11.900282	9
CCCGACT	3615	0.0	11.770401	12
CGATAAC	1160	0.0	11.6422415	10
CGAACGA	1115	0.0	11.614349	16
TCTTATA	11650	0.0	11.496996	37
AAGACGG	1440	0.0	11.434029	5
GGGTAAG	4035	0.0	11.416357	1
TATTAAG	2330	0.0	11.354077	2
TAATACT	2770	0.0	11.35379	4
>>END_MODULE