FastQCFastQC Report
Thu 9 Feb 2017
SRR2727477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13355394
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT515250.38579917597339325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT432580.3238990927560804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT361020.2703177457737301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248340.1859473408272343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176050.017.5910241
TTCGCGA1102.4594244E-413.45454522
TTATACT26950.012.1502784
TATACTG22400.012.1406255
CTTATAC63850.011.99530137
TATACCG6700.011.8731355
GTATCAA260250.011.8286272
GTACATG320250.011.8018731
TAATACT30700.011.6905554
TACATGG327300.011.4967922
TAACGCC9250.011.44
CGTATAC5100.011.2450983
ACATGGG331550.011.2099233
TACGGAT25100.011.20318727
CTCTTAT115200.011.03255237
TAGTACT12100.011.0082654
GTTACGG19350.010.99483225
CATGGGA218050.010.8514114
TATACTA18100.010.8342545
TAGGCCG4450.010.808995
Produced by FastQC (version 0.11.5)