##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727477.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13355394
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47682157486331	31.0	31.0	34.0	30.0	34.0
2	31.682657209513998	31.0	31.0	34.0	30.0	34.0
3	31.779404710935523	31.0	31.0	34.0	30.0	34.0
4	35.51290729423632	37.0	35.0	37.0	33.0	37.0
5	35.38515000006739	37.0	35.0	37.0	33.0	37.0
6	35.36485340679579	37.0	35.0	37.0	33.0	37.0
7	35.25219944840264	37.0	35.0	37.0	32.0	37.0
8	35.17970724038542	37.0	35.0	37.0	32.0	37.0
9	36.78001876994419	39.0	37.0	39.0	32.0	39.0
10	36.62429614581195	39.0	35.0	39.0	32.0	39.0
11	36.65809986586693	39.0	35.0	39.0	32.0	39.0
12	36.48714197424651	38.0	35.0	39.0	32.0	39.0
13	36.57776678097254	39.0	35.0	39.0	32.0	39.0
14	37.610567909864734	39.0	36.0	41.0	32.0	41.0
15	37.57724189941532	39.0	36.0	41.0	32.0	41.0
16	37.498638452748004	39.0	36.0	41.0	32.0	41.0
17	37.496891368386436	39.0	36.0	41.0	32.0	41.0
18	37.37561886979898	39.0	36.0	41.0	32.0	41.0
19	37.44088575746998	39.0	36.0	41.0	32.0	41.0
20	37.338688398110904	39.0	36.0	41.0	31.0	41.0
21	37.1788479621043	39.0	36.0	41.0	31.0	41.0
22	37.01282021331606	39.0	36.0	40.0	31.0	41.0
23	37.173631942269914	39.0	36.0	41.0	31.0	41.0
24	37.177751626047126	39.0	36.0	41.0	31.0	41.0
25	37.13238868130734	39.0	36.0	41.0	31.0	41.0
26	36.95348433748941	39.0	36.0	41.0	30.0	41.0
27	36.9051275462184	39.0	36.0	41.0	30.0	41.0
28	36.66615503818158	39.0	36.0	41.0	30.0	41.0
29	36.545730436705945	39.0	35.0	40.0	30.0	41.0
30	36.49444224558257	39.0	35.0	40.0	30.0	41.0
31	36.34252430141709	39.0	35.0	40.0	30.0	41.0
32	36.296810636960615	39.0	35.0	40.0	30.0	41.0
33	36.05965140376989	38.0	35.0	40.0	29.0	41.0
34	36.02239941404948	38.0	35.0	40.0	29.0	41.0
35	35.824135925903796	38.0	35.0	40.0	27.0	41.0
36	35.709715714863975	38.0	34.0	40.0	27.0	41.0
37	35.65436751622603	38.0	34.0	40.0	27.0	41.0
38	35.59987664909025	38.0	34.0	40.0	26.0	41.0
39	35.549515574007025	38.0	34.0	40.0	26.0	41.0
40	35.385256249272764	38.0	34.0	40.0	26.0	41.0
41	35.252123898403894	38.0	34.0	40.0	25.0	41.0
42	35.26590978895868	38.0	34.0	40.0	25.0	41.0
43	35.019303062118574	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	15.0
13	18.0
14	46.0
15	94.0
16	232.0
17	428.0
18	1041.0
19	2345.0
20	5215.0
21	10562.0
22	19713.0
23	33889.0
24	54524.0
25	82460.0
26	119518.0
27	166321.0
28	225453.0
29	295939.0
30	377313.0
31	470486.0
32	573922.0
33	696526.0
34	851844.0
35	1043056.0
36	1302502.0
37	1699130.0
38	2456373.0
39	2866420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14124225762265	20.351252834622475	12.588771248530744	25.91873365922413
2	18.45303852510828	22.432666531590158	34.906443044660456	24.207851898641103
3	19.393647240957474	24.575358840031225	30.806054841961235	25.224939077050067
4	14.536381330270002	16.39780151749922	35.23623488756677	33.829582264664
5	13.386755942954585	38.3572884483977	34.72066043128342	13.535295177364294
6	34.08627255773959	36.52651505451655	15.484717261055719	13.90249512668814
7	27.941931177769817	32.06418320567705	21.664422629538297	18.329462987014836
8	27.208489693377825	34.97961947060491	19.83082640616967	17.981064429847596
9	27.226954143022663	14.042041739839348	19.314278560407878	39.416725556730114
10	15.937582972093523	27.989365195815264	34.251681380571775	21.82137045151944
11	36.03492341745964	22.36514325223202	21.389215473538258	20.21071785677008
12	20.975502482367798	25.999719663830213	30.498418841106446	22.526359012695547
13	30.094806637677628	20.810235924151694	25.07741067017566	24.017546767995015
14	22.09555180476143	21.15021840613613	26.05062044594117	30.70360934316127
15	24.55512731410245	28.713409727934646	22.745057165666545	23.986405792296356
16	23.936156432374815	27.06448046384854	24.99858109764489	24.000782006131754
17	23.083384885537633	27.229604757448563	26.024009475122934	23.663000881890866
18	23.13507935445409	26.413889399294398	27.013564706514835	23.43746653973668
19	23.806343714007987	26.321986457307062	27.25228473229618	22.61938509638877
20	23.5317505421405	25.964924733781718	27.13695305432397	23.366371669753807
21	23.78150730708506	26.218657420365133	27.111614977439082	22.888220295110724
22	24.183809178523674	25.875470240713227	26.64791469274512	23.29280588801798
23	23.615791492186602	26.15126143040033	26.700956931708642	23.531990145704423
24	23.44152482510063	26.20849673173251	26.74743253549839	23.602545907668468
25	23.76544638069083	25.969544589998616	26.66533836440917	23.59967066490139
26	23.62569760203256	26.224557658126745	26.821252896020887	23.32849184381981
27	23.842973108842763	26.421481837226217	26.380052883501605	23.355492170429415
28	23.52099084459807	26.548127295982432	26.522437301363027	23.40844455805647
29	23.448967510804998	26.565558455257854	26.77833390763313	23.207140126304022
30	23.652271134793924	26.57663263247793	26.46106883855317	23.310027394174966
31	23.494177708272776	26.20780787148623	26.879169569987976	23.418844850253016
32	23.27249948597548	26.573053554241827	26.57808522908422	23.576361730698473
33	23.1772196312591	26.331256120186346	26.78198786198296	23.709536386571596
34	23.636135332285964	26.259809332468965	27.035039175931463	23.06901615931361
35	23.409530261705495	26.26871210239099	26.993220866415474	23.328536769488043
36	23.31768722060914	26.21631379800551	26.985718279820126	23.480280701565228
37	23.264779758650324	25.882965339697208	27.281778433492864	23.570476468159608
38	23.107637258773497	25.719660535660722	27.373868565764514	23.798833639801266
39	22.921180760372923	25.727208047924304	27.687958887622482	23.663652304080284
40	23.20555275269303	25.405472874854908	27.97037661337434	23.418597759077716
41	22.59666019587292	25.52693690654128	28.14712916743602	23.72927373014978
42	22.459584494474665	25.59658666752924	28.07483627963353	23.86899255836256
43	22.190951461259772	25.107735496234707	28.276020909604014	24.425292132901507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6445.0
1	5880.0
2	5315.0
3	6888.0
4	8461.0
5	8461.0
6	12466.5
7	16472.0
8	16464.0
9	16456.0
10	22555.5
11	28655.0
12	28655.0
13	49125.5
14	69596.0
15	94738.5
16	119881.0
17	112721.5
18	105562.0
19	105562.0
20	121407.0
21	137252.0
22	131581.0
23	125910.0
24	150721.0
25	175532.0
26	175532.0
27	207122.5
28	238713.0
29	267797.5
30	296882.0
31	337811.0
32	378740.0
33	378740.0
34	424009.0
35	469278.0
36	523544.0
37	577810.0
38	632867.0
39	687924.0
40	687924.0
41	743731.5
42	799539.0
43	852247.5
44	904956.0
45	958258.5
46	1011561.0
47	1011561.0
48	1063825.5
49	1116090.0
50	1122218.0
51	1128346.0
52	1101275.5
53	1074205.0
54	1074205.0
55	1022939.0
56	971673.0
57	903530.5
58	835388.0
59	757872.5
60	680357.0
61	680357.0
62	587384.5
63	494412.0
64	419385.0
65	344358.0
66	281680.0
67	219002.0
68	219002.0
69	175822.0
70	132642.0
71	105176.5
72	77711.0
73	60943.0
74	44175.0
75	44175.0
76	34559.5
77	24944.0
78	19717.0
79	14490.0
80	11113.0
81	7736.0
82	7736.0
83	5923.5
84	4111.0
85	3180.5
86	2250.0
87	1771.0
88	1292.0
89	1292.0
90	962.0
91	632.0
92	477.0
93	322.0
94	282.0
95	242.0
96	242.0
97	141.0
98	40.0
99	38.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.3355394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.908791030496886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3469261268127	34.13177495315634
2	12.223462418809577	11.223287637085216
3	4.763420105500367	6.560485746416514
4	2.3944174251774006	4.396992368489988
5	1.4505228870957878	3.3295876054316773
6	0.940536099514775	2.590732514955746
7	0.6644744732688324	2.1353653816878824
8	0.4883722736061762	1.7936464523259674
9	0.3581873052843206	1.47995515332672
>10	2.1394343647741176	17.91795518734135
>50	0.15097397254877823	4.768816279853296
>100	0.07385644831859049	6.16553042641995
>500	0.004125128702122163	1.2773710550620556
>1k	0.0011602422324466925	0.886673331001313
>5k	6.536417698767932E-5	0.17362572920014235
>10k+	6.536417698767932E-5	1.1682001782456348
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51525	0.38579917597339325	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	43258	0.3238990927560804	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36102	0.2703177457737301	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24834	0.1859473408272343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0482655921644842E-4
2	0.0	0.0	0.0	0.0	1.0482655921644842E-4
3	0.0	0.0	0.0	0.0	1.0482655921644842E-4
4	0.0	0.0	0.0	7.487611372603459E-6	1.0482655921644842E-4
5	0.0	0.0	0.0	1.4975222745206917E-5	1.272893933342588E-4
6	0.0	0.0	0.0	1.4975222745206917E-5	1.4975222745206918E-4
7	0.0	0.0	0.0	1.4975222745206917E-5	1.5723983882467264E-4
8	0.0	0.0	0.0	1.4975222745206917E-5	1.647274501972761E-4
9	0.0	0.0	0.0	2.2462834117810378E-5	1.9467789568768993E-4
10	0.0	0.0	0.0	4.4925668235620756E-5	1.9467789568768993E-4
11	0.0	0.0	0.0	5.990089098082767E-5	2.0216550706029338E-4
12	0.0	0.0	0.0	8.236372509863804E-5	2.4709117529591413E-4
13	0.0	0.0	0.0	9.733894784384497E-5	2.545787866685176E-4
14	0.0	0.0	0.0	1.0482655921644842E-4	2.920168435315349E-4
15	0.0	1.4975222745206917E-5	0.0	1.272893933342588E-4	3.5940534588496605E-4
16	0.0	1.4975222745206917E-5	0.0	2.0216550706029338E-4	4.0433101412058677E-4
17	0.0	1.4975222745206917E-5	0.0	2.9950445490413836E-4	4.267938482383972E-4
18	0.0	1.4975222745206917E-5	0.0	3.444301231397591E-4	5.091575733370352E-4
19	0.0	1.4975222745206917E-5	0.0	4.193062368657937E-4	5.166451847096387E-4
20	0.0	1.4975222745206917E-5	0.0	5.54083241572656E-4	6.064965211808801E-4
21	0.0	1.4975222745206917E-5	0.0	7.412735258877424E-4	6.139841325534836E-4
22	0.0	1.4975222745206917E-5	0.0	0.0012204806537343639	6.214717439260871E-4
23	0.0	1.4975222745206917E-5	0.0	0.0017221506156987955	6.589098007891044E-4
24	0.0	1.4975222745206917E-5	0.0	0.0030549454400222113	6.589098007891044E-4
25	0.0	1.4975222745206917E-5	0.0	0.004335326984737402	6.738850235343113E-4
26	0.0	1.4975222745206917E-5	0.0	0.007487611372603459	6.963478576521217E-4
27	0.0	1.4975222745206917E-5	0.0	0.029800693262961767	7.33785914515139E-4
28	7.487611372603459E-6	1.4975222745206917E-5	0.0	0.057152937607082205	7.33785914515139E-4
29	7.487611372603459E-6	1.4975222745206917E-5	0.0	0.09108679234772107	7.412735258877424E-4
30	7.487611372603459E-6	1.4975222745206917E-5	0.0	0.1395990264308189	7.412735258877424E-4
31	7.487611372603459E-6	1.4975222745206917E-5	0.0	0.2844169179883424	7.412735258877424E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17605	0.0	17.591024	1
TTCGCGA	110	2.4594244E-4	13.454545	22
TTATACT	2695	0.0	12.150278	4
TATACTG	2240	0.0	12.140625	5
CTTATAC	6385	0.0	11.995301	37
TATACCG	670	0.0	11.873135	5
GTATCAA	26025	0.0	11.828627	2
GTACATG	32025	0.0	11.801873	1
TAATACT	3070	0.0	11.690555	4
TACATGG	32730	0.0	11.496792	2
TAACGCC	925	0.0	11.4	4
CGTATAC	510	0.0	11.245098	3
ACATGGG	33155	0.0	11.209923	3
TACGGAT	2510	0.0	11.203187	27
CTCTTAT	11520	0.0	11.032552	37
TAGTACT	1210	0.0	11.008265	4
GTTACGG	1935	0.0	10.994832	25
CATGGGA	21805	0.0	10.851411	4
TATACTA	1810	0.0	10.834254	5
TAGGCCG	445	0.0	10.80899	5
>>END_MODULE