FastQCFastQC Report
Thu 9 Feb 2017
SRR2727476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054456
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73020.10350904449613124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.024.415361
GTATCAA49350.015.5947312
ATACCGT5550.013.6666676
TATACCG3200.013.2968755
TATACTG12100.012.6900835
GGGTAAG28400.012.5721831
TACCGTC4500.012.3333327
GTATTAC6300.011.7460321
TTATACT13650.011.5201464
TATACAG14650.011.4914675
TCTTATA24900.011.44176737
TCGTTAC8100.011.41975323
CCGATAA4750.011.2947379
ATCAACG71400.011.27100852
TTACACT12350.011.23481754
TCAACGC72350.011.1741533
TACACCG5150.011.1359225
TTTATAC15150.011.1122113
GTTACGG12350.011.0850225
ATAGTAC6850.011.0729933
Produced by FastQC (version 0.11.5)