##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7054456
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426217556676235	31.0	31.0	34.0	30.0	34.0
2	31.639488147633212	31.0	31.0	34.0	30.0	34.0
3	31.734624611734766	31.0	31.0	34.0	30.0	34.0
4	35.48397154932996	37.0	35.0	37.0	33.0	37.0
5	35.339207020357065	37.0	35.0	37.0	33.0	37.0
6	35.314963336648496	37.0	35.0	37.0	33.0	37.0
7	35.20893092252613	37.0	35.0	37.0	32.0	37.0
8	35.10563408432911	37.0	35.0	37.0	32.0	37.0
9	36.68648468429033	39.0	35.0	39.0	32.0	39.0
10	36.54316023801127	38.0	35.0	39.0	32.0	39.0
11	36.567125941390806	38.0	35.0	39.0	32.0	39.0
12	36.3929132168377	38.0	35.0	39.0	32.0	39.0
13	36.48037538259506	38.0	35.0	39.0	32.0	39.0
14	37.51336474421274	39.0	36.0	41.0	32.0	41.0
15	37.48069220362279	39.0	36.0	41.0	32.0	41.0
16	37.41008987794381	39.0	36.0	41.0	32.0	41.0
17	37.38616826017485	39.0	36.0	41.0	32.0	41.0
18	37.239392661886335	39.0	36.0	41.0	31.0	41.0
19	37.291711366546195	39.0	36.0	41.0	31.0	41.0
20	37.18539317560418	39.0	36.0	40.0	31.0	41.0
21	36.99959458248801	39.0	36.0	40.0	31.0	41.0
22	36.802302969924256	39.0	36.0	40.0	30.0	41.0
23	37.00093288554071	39.0	36.0	40.0	31.0	41.0
24	36.99089058036509	39.0	36.0	41.0	31.0	41.0
25	36.94147316249474	39.0	36.0	41.0	30.0	41.0
26	36.77558737342752	39.0	36.0	40.0	30.0	41.0
27	36.653553441966324	39.0	36.0	40.0	30.0	41.0
28	36.41085974595348	39.0	35.0	40.0	29.0	41.0
29	36.34635654400566	39.0	35.0	40.0	29.0	41.0
30	36.33419926355767	39.0	35.0	40.0	29.0	41.0
31	36.19937327555803	38.0	35.0	40.0	29.0	41.0
32	36.156092829836915	38.0	35.0	40.0	29.0	41.0
33	35.95348046681417	38.0	35.0	40.0	27.0	41.0
34	35.93893505041353	38.0	35.0	40.0	27.0	41.0
35	35.74943227372883	38.0	34.0	40.0	27.0	41.0
36	35.650408479406494	38.0	34.0	40.0	27.0	41.0
37	35.61988181654262	38.0	34.0	40.0	27.0	41.0
38	35.59322462284831	38.0	34.0	40.0	26.0	41.0
39	35.56981133626746	38.0	34.0	40.0	26.0	41.0
40	35.40908370539132	38.0	34.0	40.0	26.0	41.0
41	35.2803406811241	38.0	34.0	40.0	25.0	41.0
42	35.33544967889799	38.0	34.0	40.0	26.0	41.0
43	35.100211554228984	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	4.0
14	12.0
15	35.0
16	101.0
17	222.0
18	530.0
19	1213.0
20	2930.0
21	5772.0
22	10979.0
23	19055.0
24	31001.0
25	46396.0
26	66923.0
27	93746.0
28	125504.0
29	163436.0
30	208211.0
31	257745.0
32	313327.0
33	376576.0
34	456494.0
35	555953.0
36	687924.0
37	882795.0
38	1297831.0
39	1449735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57231344273747	20.4731732680734	12.571543432973428	26.382969856215706
2	18.917688337697477	22.282455231133344	34.45986480034747	24.33999163082171
3	19.5485945337245	24.271736899344187	30.634764750109717	25.544903816821595
4	15.037715168965546	16.518126982434932	35.00169254723539	33.44246530136413
5	13.763187409489833	37.92686494890605	34.7010456936722	13.608901947931917
6	34.48523883344088	36.30231728711612	15.313271498184978	13.89917238125803
7	28.22265813267529	31.741426978919424	21.4400798587446	18.595835029660684
8	27.046394505827237	34.798487650925885	19.837532475927272	18.317585367319605
9	27.254844314005215	14.0836940509658	19.008907277896412	39.652554357132566
10	15.974711586548985	27.64403095008318	34.38652108681378	21.99473637655405
11	36.5015247100556	22.042847811369153	21.321899236454236	20.13372824212101
12	21.093093500051598	25.567456937856015	30.061141496948878	23.27830806514351
13	30.403081399898163	20.600270240540162	24.504001442492516	24.492646917069155
14	22.432984201758437	20.97254557970168	25.11640869260507	31.478061525934812
15	25.096307922255097	28.544851651211662	21.89434592830404	24.464494498229207
16	24.516348248539646	26.861631853682265	24.205778588738806	24.41624130903928
17	23.57087491934176	26.840652206208386	25.25527127818219	24.333201596267664
18	23.676269297023044	26.44641911438671	25.974972414598657	23.90233917399159
19	24.467258708538264	26.132957665339468	26.102508825627375	23.297274800494893
20	24.178235713710595	25.827321057782488	25.913649472050004	24.080793756456913
21	24.345619279502202	25.997766518070282	26.008369745307082	23.648244457120434
22	24.87546878171754	25.612123741362907	25.450141017252076	24.062266459667477
23	24.396012959751964	25.847634459694696	25.560936236614136	24.195416343939208
24	24.16264273248001	25.889239368705397	25.573155463723925	24.374962435090673
25	24.56030628017242	25.54301848363644	25.568633499167053	24.32804173702409
26	24.439389798447962	25.80967263811696	25.700578471252776	24.0503590921823
27	24.67599202546589	26.032737322339244	25.22526187703205	24.06600877516282
28	24.29104384519515	26.128308121845258	25.314978220857853	24.26566981210174
29	24.223753043466427	26.09953198375608	25.66006507092822	24.01664990184927
30	24.5020877584324	26.004967640311317	25.343924464196814	24.14902013705947
31	24.373870926404532	25.67521861359685	25.79009635895383	24.160814101044785
32	24.191447221444147	26.043624058325687	25.49025750532713	24.274671214903034
33	24.011560919793105	25.84285733726314	25.637554476206244	24.508027266737507
34	24.471100252095983	25.766295799420963	25.98203178246487	23.78057216601819
35	24.279533389959482	25.718552925980404	25.89479614019848	24.10711754386164
36	24.173075854467022	25.7272282937196	25.950633188441465	24.149062663371918
37	24.13046448939507	25.479243190403345	26.259771129056585	24.130521191145
38	24.11261761360479	25.33979941188945	26.169771276481136	24.377811698024622
39	23.657345087984105	25.487620873955414	26.57837826190992	24.276655776150562
40	24.15117480355679	24.984236913519624	26.75409698494115	24.11049129798244
41	23.588792672319453	25.15342075987149	26.881307360907776	24.376479206901283
42	23.435357737010477	25.132639568522364	26.902485464506405	24.529517229960753
43	23.27898848614266	24.56791849010044	27.06239857474481	25.090694449012084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	677.0
1	663.5
2	650.0
3	854.0
4	1058.0
5	1058.0
6	1604.5
7	2151.0
8	2222.5
9	2294.0
10	3325.0
11	4356.0
12	4356.0
13	7338.5
14	10321.0
15	14182.0
16	18043.0
17	19761.5
18	21480.0
19	21480.0
20	27910.0
21	34340.0
22	39114.0
23	43888.0
24	56654.5
25	69421.0
26	69421.0
27	86538.0
28	103655.0
29	120607.0
30	137559.0
31	163590.5
32	189622.0
33	189622.0
34	218081.0
35	246540.0
36	280531.5
37	314523.0
38	346531.5
39	378540.0
40	378540.0
41	412912.5
42	447285.0
43	477725.0
44	508165.0
45	536598.5
46	565032.0
47	565032.0
48	594206.5
49	623381.0
50	629584.5
51	635788.0
52	620646.5
53	605505.0
54	605505.0
55	571627.5
56	537750.0
57	496500.5
58	455251.0
59	410704.0
60	366157.0
61	366157.0
62	316562.5
63	266968.0
64	226298.0
65	185628.0
66	151331.5
67	117035.0
68	117035.0
69	93658.5
70	70282.0
71	55450.5
72	40619.0
73	31952.0
74	23285.0
75	23285.0
76	18142.5
77	13000.0
78	10003.0
79	7006.0
80	5331.0
81	3656.0
82	3656.0
83	2710.5
84	1765.0
85	1339.0
86	913.0
87	701.0
88	489.0
89	489.0
90	349.5
91	210.0
92	159.5
93	109.0
94	78.5
95	48.0
96	48.0
97	28.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7054456.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.96453404323884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72660648993912	40.6378896145857
2	11.922759098596627	12.629667603339131
3	4.262143052839014	6.772272624577376
4	2.035047635800127	4.3114139914379415
5	1.20446287261602	3.1896907410244224
6	0.7834013808597119	2.4895493463638716
7	0.5240457898405019	1.9429088753356274
8	0.40325006378892625	1.7086361385189455
9	0.31466115687454904	1.4999293399830271
>10	1.672721798584732	16.085005414262753
>50	0.10556601452459355	3.822717135140851
>100	0.043693656445984864	4.026455465818995
>500	0.0014526973231320719	0.5098838488478658
>1k	1.0759540980202406E-4	0.09867369594457602
>5k	8.06965571787871E-5	0.27530616481883324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7302	0.10350904449613124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.417543748235158E-5
2	0.0	0.0	0.0	0.0	1.417543748235158E-5
3	0.0	0.0	0.0	0.0	1.417543748235158E-5
4	0.0	0.0	0.0	1.417543748235158E-5	1.417543748235158E-5
5	0.0	0.0	0.0	1.417543748235158E-5	1.417543748235158E-5
6	0.0	0.0	0.0	1.417543748235158E-5	8.505262489410948E-5
7	0.0	0.0	0.0	1.417543748235158E-5	1.1340349985881264E-4
8	0.0	0.0	0.0	1.417543748235158E-5	1.2757893734116423E-4
9	0.0	0.0	0.0	4.252631244705474E-5	1.8428068727057055E-4
10	0.0	0.0	0.0	5.670174992940632E-5	1.8428068727057055E-4
11	0.0	0.0	0.0	5.670174992940632E-5	1.8428068727057055E-4
12	0.0	0.0	0.0	5.670174992940632E-5	2.2680699971762528E-4
13	0.0	0.0	0.0	7.08771874117579E-5	2.2680699971762528E-4
14	0.0	0.0	0.0	8.505262489410948E-5	3.2603506209408635E-4
15	0.0	0.0	0.0	9.922806237646106E-5	3.5438593705878953E-4
16	0.0	0.0	0.0	1.1340349985881264E-4	3.685613745411411E-4
17	0.0	0.0	0.0	1.1340349985881264E-4	3.685613745411411E-4
18	0.0	0.0	0.0	1.1340349985881264E-4	3.8273681202349267E-4
19	0.0	0.0	0.0	1.1340349985881264E-4	3.8273681202349267E-4
20	0.0	0.0	0.0	1.2757893734116423E-4	3.9691224950584423E-4
21	0.0	0.0	0.0	1.7010524978821896E-4	4.1108768698819585E-4
22	0.0	0.0	0.0	3.5438593705878953E-4	4.1108768698819585E-4
23	0.0	0.0	0.0	5.670174992940632E-4	5.528420618117117E-4
24	0.0	0.0	0.0	9.497543113175559E-4	5.528420618117117E-4
25	0.0	0.0	0.0	0.0013608419983057517	5.528420618117117E-4
26	0.0	0.0	0.0	0.0025515787468232843	5.953683742587663E-4
27	0.0	0.0	0.0	0.010121262362399028	5.953683742587663E-4
28	0.0	0.0	0.0	0.020072419475009838	5.953683742587663E-4
29	0.0	0.0	0.0	0.033014593896396834	5.953683742587663E-4
30	0.0	0.0	0.0	0.05247746955966555	5.953683742587663E-4
31	0.0	0.0	0.0	0.12470132353224685	6.095438117411179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3190	0.0	24.41536	1
GTATCAA	4935	0.0	15.594731	2
ATACCGT	555	0.0	13.666667	6
TATACCG	320	0.0	13.296875	5
TATACTG	1210	0.0	12.690083	5
GGGTAAG	2840	0.0	12.572183	1
TACCGTC	450	0.0	12.333332	7
GTATTAC	630	0.0	11.746032	1
TTATACT	1365	0.0	11.520146	4
TATACAG	1465	0.0	11.491467	5
TCTTATA	2490	0.0	11.441767	37
TCGTTAC	810	0.0	11.419753	23
CCGATAA	475	0.0	11.294737	9
ATCAACG	7140	0.0	11.2710085	2
TTACACT	1235	0.0	11.2348175	4
TCAACGC	7235	0.0	11.174153	3
TACACCG	515	0.0	11.135922	5
TTTATAC	1515	0.0	11.112211	3
GTTACGG	1235	0.0	11.08502	25
ATAGTAC	685	0.0	11.072993	3
>>END_MODULE