FastQCFastQC Report
Thu 9 Feb 2017
SRR2727475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11456511
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT660200.5762661948301713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT554880.4843359378784693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT462420.4036307388872581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346870.30277106180057783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206250.017.7061821
TCTTATA108100.012.54440437
AAGACGG20400.012.424025
CTTATAC74750.012.20133737
CGTATAC3800.012.1710533
GTACTAG9150.011.9289611
ACGAACG14250.011.81403515
GTATCAA308250.011.7931882
CGAACGA13750.011.70545416
ACCGAAC12300.011.5813018
GTACATG373250.011.4147351
TATACCG6550.011.2977095
ACGGACC19150.011.2062658
TAAGGCG7100.011.2042265
TACATGG377600.011.1950472
TGCGACG5000.011.122
CAAGACG23700.011.0843884
AACGCAG379150.011.0077815
CTCTTAT151150.010.99106837
ACATGGG382800.010.9414843
Produced by FastQC (version 0.11.5)