FastQCFastQC Report
Thu 9 Feb 2017
SRR2727474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341091
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167800.2285763791785172No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC151670.20660416823602923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146060.19896225234096676No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA134640.18340598148149914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122650.16707325927440486No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG122310.16661011285652227No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT118130.16091613630726004No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG112840.15371012292314587No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA107340.1462180485162219No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC105410.14358901149706496No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC99090.13497993690583593No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG89480.12188923962391966No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC88910.121112788276293No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG86020.1171760437242911No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC85150.1159909337726504No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT81530.11105978661754771No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT80310.10939790829455731No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT77090.10501163927813999No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG76870.10471195630186304No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG76610.10435778551171754No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG75870.10334976095514958No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA75830.10329527314128105No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC74730.10179685825989625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC20150.017.9950373
AAGACGG26150.017.6864245
CAAGACG26950.017.161414
GGTATCA100650.016.1748641
CGCAATA18100.015.02486136
TTCGGGC12000.014.95416635
CGCTTCG11850.014.831223532
TCTAGCG17950.014.73816228
CTAGCGG18050.014.65650929
CGCAAGA32400.014.5601852
ATCTCCG21450.014.489511510
TTATACA24250.014.4185562
ACGGACC32250.014.0542648
TATGCCG7900.014.05063233
AGACGGA33950.013.9499266
GCGCAAG33250.013.85413551
TACACAT25850.013.8123795
TATTCCG2551.8189894E-1213.7843135
CGAGCCG26200.013.62786315
GCGCAAT20000.013.59750135
Produced by FastQC (version 0.11.5)