##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7341091
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32394326674332	31.0	31.0	34.0	30.0	34.0
2	31.6039760302658	31.0	31.0	34.0	30.0	34.0
3	31.70789968412052	31.0	31.0	34.0	30.0	34.0
4	35.482174107363605	37.0	35.0	37.0	33.0	37.0
5	35.29505927661161	37.0	35.0	37.0	33.0	37.0
6	35.28921613967188	37.0	35.0	37.0	32.0	37.0
7	35.21816144221615	37.0	35.0	37.0	32.0	37.0
8	35.193227954809444	37.0	35.0	37.0	32.0	37.0
9	36.82379063275472	39.0	37.0	39.0	32.0	39.0
10	36.60649064287583	39.0	35.0	39.0	32.0	39.0
11	36.717163293575844	39.0	35.0	39.0	32.0	39.0
12	36.5459087756847	39.0	35.0	39.0	32.0	39.0
13	36.64870521289002	39.0	35.0	39.0	32.0	39.0
14	37.7607489132065	40.0	37.0	41.0	32.0	41.0
15	37.717345827752304	40.0	37.0	41.0	32.0	41.0
16	37.6897210509991	40.0	37.0	41.0	32.0	41.0
17	37.626558232284545	39.0	36.0	41.0	32.0	41.0
18	37.6093004432175	39.0	36.0	41.0	32.0	41.0
19	37.60117971565807	39.0	36.0	41.0	32.0	41.0
20	37.56104140379134	39.0	36.0	41.0	32.0	41.0
21	37.50233963861775	39.0	36.0	41.0	32.0	41.0
22	37.41737229520789	39.0	36.0	41.0	31.0	41.0
23	37.321275134717716	39.0	36.0	41.0	31.0	41.0
24	37.22802128457473	39.0	36.0	41.0	31.0	41.0
25	37.13823149719844	39.0	36.0	41.0	31.0	41.0
26	36.953309800954656	39.0	36.0	41.0	31.0	41.0
27	36.866916511455855	39.0	36.0	40.0	30.0	41.0
28	36.6889207612329	39.0	35.0	40.0	30.0	41.0
29	36.55967021795534	39.0	35.0	40.0	30.0	41.0
30	36.6900067033633	39.0	35.0	40.0	30.0	41.0
31	36.71599085204093	39.0	35.0	40.0	30.0	41.0
32	36.69644961491419	39.0	35.0	40.0	30.0	41.0
33	36.57786397144512	39.0	35.0	40.0	30.0	41.0
34	36.549677289111386	39.0	35.0	40.0	30.0	41.0
35	36.44220225576825	39.0	35.0	40.0	30.0	41.0
36	36.365129515490274	38.0	35.0	40.0	30.0	41.0
37	36.29665767663144	38.0	35.0	40.0	30.0	41.0
38	36.16607027484062	38.0	35.0	40.0	29.0	41.0
39	36.04770571567632	38.0	35.0	40.0	29.0	41.0
40	35.92969015095985	38.0	35.0	40.0	28.0	41.0
41	35.78439812284032	38.0	34.0	40.0	28.0	41.0
42	35.651386421990956	38.0	34.0	40.0	27.0	41.0
43	35.50028177010747	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	9.0
13	10.0
14	20.0
15	58.0
16	216.0
17	433.0
18	609.0
19	1102.0
20	2278.0
21	4352.0
22	8011.0
23	14086.0
24	23089.0
25	36443.0
26	53613.0
27	78313.0
28	108296.0
29	147583.0
30	193043.0
31	244777.0
32	302745.0
33	371602.0
34	459567.0
35	568158.0
36	715880.0
37	926419.0
38	1354418.0
39	1725958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59907689470135	19.189777105337612	12.09982276476344	26.111323235197602
2	19.4096626782041	21.59782517339725	33.15289239705652	25.839619751342134
3	20.33306765983421	23.799718597685278	29.28553807601622	26.58167566646429
4	14.804189186593655	15.876781802595827	34.17261821165274	35.146410799157785
5	14.81757956685185	36.97637040597917	33.20352519809385	15.002524829075133
6	34.7092550684905	34.81584685437083	15.87338993618251	14.60150814095616
7	29.124322256732682	31.085515763256446	21.0033222582311	18.786839721779774
8	28.222630668929185	32.55836223798343	19.949459828246237	19.26954726484115
9	27.479784680505936	13.955269046521831	19.09585373618172	39.46909253679051
10	17.79924809541252	25.70796357108228	31.72995675983311	24.762831573672088
11	37.41170079488185	21.319147249366612	20.608081278382194	20.661070677369345
12	21.74642706377022	25.39675642217213	28.72227030015021	24.13454621390744
13	30.026681320256078	19.086781515172607	25.32980452088116	25.556732643690154
14	23.133809402444406	20.638635320008973	23.919387458894054	32.308167818652564
15	25.92564238748709	27.476433679953022	21.343680387560923	25.254243544998968
16	26.180141344113565	25.21686490468515	23.846850556681563	24.756143194519726
17	24.71842400536923	26.037778308428543	24.00068327718591	25.243114409016314
18	25.483964168268724	23.735014318716388	25.625564374559584	25.155457138455304
19	25.058332610234636	25.058291744374234	24.936034712006702	24.947340933384424
20	24.82009826604792	23.690443286971924	25.16151890774818	26.327939539231977
21	26.120858602624597	24.49285807790695	25.219780002727116	24.166503316741338
22	25.969736650860202	23.96955711351351	25.277360000032694	24.78334623559359
23	24.64392554185747	23.877731525191557	25.739620990939905	25.73872194201107
24	24.419912517090445	24.88442113031973	25.625864057535864	25.069802295053968
25	25.277673304962438	24.10893694138923	24.90558964600766	25.707800107640676
26	24.95115508035522	25.388256323208637	25.263901510007162	24.39668708642898
27	26.5314379020775	24.048005943530736	24.827985377105392	24.592570777286372
28	24.72503065280079	24.66152510573701	25.589779502801424	25.023664738660777
29	24.609748060608432	24.5883752156185	25.846253642680633	24.955623081092444
30	24.229028083155487	25.143333599869557	26.144887728540624	24.482750588434335
31	25.3124637741175	24.908273170840683	24.832235426587136	24.94702762845468
32	25.318116884806358	24.388527536302167	24.535998804537364	25.75735677435411
33	24.1275172859184	24.26655656495744	25.40300889881354	26.202917250310616
34	25.943405414808236	24.504859018911493	25.322244336706902	24.22949122957337
35	25.6006498216682	23.942435804160446	26.134889214695743	24.322025159475615
36	24.125841785641946	25.416453766885606	25.385218627585466	25.072485819886992
37	25.650669634799517	24.593715021377612	25.520988637792392	24.23462670603048
38	24.567492760953378	23.922792947260838	25.797705000523763	25.71200929126202
39	25.570231999576087	24.197670346274144	24.98995040382962	25.24214725032015
40	25.25639581364677	24.136289823951234	25.94104881685842	24.66626554554357
41	24.151355704485887	25.253385361930537	26.0219632204532	24.573295713130378
42	25.897022663252645	24.65570853160654	26.036960991220514	23.4103078139203
43	24.67853892561746	23.553569898534153	25.777857814322147	25.990033361526237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	708.0
1	944.0
2	1180.0
3	2415.5
4	3651.0
5	3651.0
6	4845.5
7	6040.0
8	5071.5
9	4103.0
10	5735.5
11	7368.0
12	7368.0
13	12713.5
14	18059.0
15	24688.5
16	31318.0
17	30101.0
18	28884.0
19	28884.0
20	35504.5
21	42125.0
22	39845.5
23	37566.0
24	45853.0
25	54140.0
26	54140.0
27	66434.5
28	78729.0
29	91796.5
30	104864.0
31	121312.5
32	137761.0
33	137761.0
34	162894.0
35	188027.0
36	225247.0
37	262467.0
38	298441.0
39	334415.0
40	334415.0
41	370635.0
42	406855.0
43	429445.5
44	452036.0
45	497982.5
46	543929.0
47	543929.0
48	599448.0
49	654967.0
50	651131.0
51	647295.0
52	634280.5
53	621266.0
54	621266.0
55	598459.0
56	575652.0
57	548355.0
58	521058.0
59	488792.5
60	456527.0
61	456527.0
62	395211.5
63	333896.0
64	311319.0
65	288742.0
66	250286.5
67	211831.0
68	211831.0
69	165483.5
70	119136.0
71	101117.0
72	83098.0
73	57287.0
74	31476.0
75	31476.0
76	24634.0
77	17792.0
78	15757.5
79	13723.0
80	10734.0
81	7745.0
82	7745.0
83	6629.0
84	5513.0
85	4841.0
86	4169.0
87	2795.5
88	1422.0
89	1422.0
90	1086.0
91	750.0
92	552.5
93	355.0
94	383.5
95	412.0
96	412.0
97	213.5
98	15.0
99	20.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7341091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.6735209518769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94382361593657	32.89867087480007
2	10.83185371578073	9.028029655445078
3	3.6598929081724028	4.5756187137104485
4	1.8165428899002678	3.0280695272896727
5	1.0766545956579845	2.2433993925043785
6	0.6986322913863956	1.7468680459649235
7	0.4851157430349671	1.4151536757017122
8	0.35977342755013186	1.1994420376751187
9	0.2760094438385844	1.0352056806650825
>10	1.6368414738352717	12.569880124603197
>50	0.11362029219651071	3.254235642337154
>100	0.07408072539245657	6.345979286746789
>500	0.01204897573868698	3.5356442904600134
>1k	0.013514243869577825	11.734885369151659
>5k	0.0012700132790714512	3.639851569374915
>10k+	3.256444305311413E-4	1.7490661135697596
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16780	0.2285763791785172	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	15167	0.20660416823602923	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14606	0.19896225234096676	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	13464	0.18340598148149914	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12265	0.16707325927440486	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	12231	0.16661011285652227	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	11813	0.16091613630726004	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	11284	0.15371012292314587	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	10734	0.1462180485162219	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	10541	0.14358901149706496	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	9909	0.13497993690583593	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	8948	0.12188923962391966	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	8891	0.121112788276293	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	8602	0.1171760437242911	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	8515	0.1159909337726504	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	8153	0.11105978661754771	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	8031	0.10939790829455731	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	7709	0.10501163927813999	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	7687	0.10471195630186304	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	7661	0.10435778551171754	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	7587	0.10334976095514958	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	7583	0.10329527314128105	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	7473	0.10179685825989625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3621953467134517E-5	4.086586040140355E-5
2	0.0	0.0	0.0	1.3621953467134517E-5	4.086586040140355E-5
3	0.0	0.0	0.0	2.7243906934269035E-5	4.086586040140355E-5
4	0.0	0.0	0.0	8.17317208028071E-5	4.086586040140355E-5
5	0.0	0.0	0.0	1.2259758120421066E-4	4.086586040140355E-5
6	0.0	0.0	0.0	1.2259758120421066E-4	5.448781386853807E-5
7	0.0	0.0	0.0	1.2259758120421066E-4	5.448781386853807E-5
8	0.0	0.0	0.0	1.3621953467134518E-4	5.448781386853807E-5
9	0.0	0.0	0.0	1.7708539507274872E-4	5.448781386853807E-5
10	0.0	0.0	0.0	1.9070734853988324E-4	5.448781386853807E-5
11	0.0	0.0	0.0	2.0432930200701776E-4	5.448781386853807E-5
12	0.0	0.0	0.0	2.0432930200701776E-4	6.810976733567259E-5
13	0.0	0.0	0.0	2.0432930200701776E-4	1.0897562773707614E-4
14	0.0	0.0	0.0	2.0432930200701776E-4	1.7708539507274872E-4
15	0.0	0.0	0.0	2.0432930200701776E-4	1.7708539507274872E-4
16	1.3621953467134517E-5	0.0	0.0	2.315732089412868E-4	1.7708539507274872E-4
17	1.3621953467134517E-5	0.0	0.0	2.315732089412868E-4	1.7708539507274872E-4
18	1.3621953467134517E-5	0.0	0.0	2.5881711587555584E-4	1.7708539507274872E-4
19	1.3621953467134517E-5	0.0	0.0	2.7243906934269036E-4	1.9070734853988324E-4
20	1.3621953467134517E-5	0.0	0.0	3.1330492974409387E-4	2.0432930200701776E-4
21	1.3621953467134517E-5	0.0	0.0	3.6779274361263195E-4	2.5881711587555584E-4
22	1.3621953467134517E-5	0.0	0.0	5.176342317511117E-4	2.5881711587555584E-4
23	1.3621953467134517E-5	0.0	0.0	7.219635337581294E-4	3.269268832112284E-4
24	1.3621953467134517E-5	0.0	0.0	0.0010080245565679542	3.269268832112284E-4
25	1.3621953467134517E-5	0.0	0.0	0.0012532197189763755	3.269268832112284E-4
26	1.3621953467134517E-5	0.0	0.0	0.0023021101359457334	3.405488366783629E-4
27	1.3621953467134517E-5	0.0	0.0	0.005394293572985269	3.405488366783629E-4
28	1.3621953467134517E-5	0.0	0.0	0.014371160907826915	3.405488366783629E-4
29	1.3621953467134517E-5	0.0	0.0	0.02856523642058108	3.5417079014549743E-4
30	1.3621953467134517E-5	0.0	0.0	0.049733752108508125	3.5417079014549743E-4
31	1.3621953467134517E-5	0.0	0.0	0.10179685825989625	3.5417079014549743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2015	0.0	17.995037	3
AAGACGG	2615	0.0	17.686424	5
CAAGACG	2695	0.0	17.16141	4
GGTATCA	10065	0.0	16.174864	1
CGCAATA	1810	0.0	15.024861	36
TTCGGGC	1200	0.0	14.954166	35
CGCTTCG	1185	0.0	14.8312235	32
TCTAGCG	1795	0.0	14.738162	28
CTAGCGG	1805	0.0	14.656509	29
CGCAAGA	3240	0.0	14.560185	2
ATCTCCG	2145	0.0	14.4895115	10
TTATACA	2425	0.0	14.418556	2
ACGGACC	3225	0.0	14.054264	8
TATGCCG	790	0.0	14.050632	33
AGACGGA	3395	0.0	13.949926	6
GCGCAAG	3325	0.0	13.8541355	1
TACACAT	2585	0.0	13.812379	5
TATTCCG	255	1.8189894E-12	13.784313	5
CGAGCCG	2620	0.0	13.627863	15
GCGCAAT	2000	0.0	13.597501	35
>>END_MODULE