##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6340013
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30780599345774	31.0	31.0	34.0	30.0	34.0
2	31.58110148985499	31.0	31.0	34.0	30.0	34.0
3	31.681015953752777	31.0	31.0	34.0	30.0	34.0
4	35.45488723761292	37.0	35.0	37.0	33.0	37.0
5	35.268755600343404	37.0	35.0	37.0	33.0	37.0
6	35.2781921740539	37.0	35.0	37.0	32.0	37.0
7	35.20689168933881	37.0	35.0	37.0	32.0	37.0
8	35.193051023712414	37.0	35.0	37.0	32.0	37.0
9	36.81290038364275	39.0	37.0	39.0	32.0	39.0
10	36.59704420164438	39.0	35.0	39.0	32.0	39.0
11	36.71564995213732	39.0	35.0	39.0	32.0	39.0
12	36.55222016106276	39.0	35.0	39.0	32.0	39.0
13	36.65474471424585	39.0	35.0	39.0	32.0	39.0
14	37.74102135121805	40.0	37.0	41.0	32.0	41.0
15	37.7053383013568	40.0	37.0	41.0	32.0	41.0
16	37.649095672201305	39.0	36.0	41.0	32.0	41.0
17	37.59053080805986	39.0	36.0	41.0	32.0	41.0
18	37.60513472133259	39.0	36.0	41.0	32.0	41.0
19	37.61455094808165	39.0	36.0	41.0	32.0	41.0
20	37.58846204258572	39.0	36.0	41.0	32.0	41.0
21	37.53972838856955	39.0	36.0	41.0	32.0	41.0
22	37.47170723466971	39.0	36.0	41.0	32.0	41.0
23	37.34593130960457	39.0	36.0	41.0	31.0	41.0
24	37.29247873781962	39.0	36.0	41.0	31.0	41.0
25	37.19430843438334	39.0	36.0	41.0	31.0	41.0
26	36.99968832871478	39.0	36.0	41.0	31.0	41.0
27	36.93226575402921	39.0	36.0	40.0	31.0	41.0
28	36.76448849552832	39.0	35.0	40.0	30.0	41.0
29	36.6431644540792	39.0	35.0	40.0	30.0	41.0
30	36.75026407674559	39.0	35.0	40.0	30.0	41.0
31	36.72472201555423	39.0	35.0	40.0	30.0	41.0
32	36.69564242218431	39.0	35.0	40.0	30.0	41.0
33	36.576767113884465	39.0	35.0	40.0	30.0	41.0
34	36.553520473853915	39.0	35.0	40.0	30.0	41.0
35	36.45546610077929	39.0	35.0	40.0	30.0	41.0
36	36.37969133501777	38.0	35.0	40.0	30.0	41.0
37	36.30823548784522	38.0	35.0	40.0	30.0	41.0
38	36.1921335177073	38.0	35.0	40.0	29.0	41.0
39	36.05652890617101	38.0	35.0	40.0	29.0	41.0
40	35.94916950485748	38.0	35.0	40.0	28.0	41.0
41	35.80226365466443	38.0	34.0	40.0	28.0	41.0
42	35.68340948196794	38.0	34.0	40.0	27.0	41.0
43	35.50885495029742	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	7.0
14	13.0
15	21.0
16	88.0
17	181.0
18	335.0
19	751.0
20	1699.0
21	3340.0
22	6444.0
23	11328.0
24	18742.0
25	29944.0
26	45320.0
27	66442.0
28	92769.0
29	126053.0
30	166794.0
31	211782.0
32	261834.0
33	322476.0
34	400506.0
35	495732.0
36	619758.0
37	806066.0
38	1161442.0
39	1490142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76988075576501	19.024472031839682	12.39145724149146	25.814189970903843
2	18.763557740339017	22.12265179897896	33.766728869483394	25.347061591198628
3	20.596740101321558	24.394934205970873	29.669639478657224	25.338686214050348
4	14.975679071951431	16.241070799066186	33.636445224954585	35.1468049040278
5	14.290096881504816	37.943502639505624	33.12843364832217	14.63796683066738
6	34.18515072445435	34.2015860219845	16.357632074256	15.255631179305155
7	28.242197610635817	31.96909848607566	21.03796948050422	18.75073442278431
8	28.346850392893515	33.03942752167858	20.285683956799456	18.328038128628442
9	28.323064952705934	13.90964024837173	19.02948779442566	38.73780700449668
10	17.789032924695896	26.707894762991813	31.875155461037696	23.627916851274595
11	36.46599778265439	21.83411926757879	20.416409240801244	21.28347370896558
12	21.41686460264356	25.877880692042744	29.038442034740306	23.66681267057339
13	30.323108170282932	19.551726471223326	25.347850233114666	24.77731512537908
14	22.919243225526508	21.05730698028537	24.83050113619641	31.19294865799171
15	26.51237465916868	27.1023103580387	22.123724351984766	24.261590630807856
16	25.178544586580497	25.55207694369081	25.198639182601045	24.070739287127648
17	24.373356963148183	26.246602333465248	24.635233397786408	24.744807305600162
18	24.709618103306727	24.169035615542114	26.524425107645676	24.596921173505482
19	24.6420157182643	25.725672802248194	26.096381821299104	23.535929658188397
20	24.11066034091728	23.75518788368415	26.506570254666673	25.627581520731894
21	24.913497811439818	25.02958590148001	26.89808364746255	23.158832639617614
22	25.315105820760937	24.301716731495663	25.86512046584132	24.518056981902088
23	24.009603765796694	24.364476855173642	26.81934563856573	24.80657374046394
24	23.488895054316135	25.34479030248045	26.73300512159833	24.433309521605082
25	24.598861232618923	24.352473725211603	25.701004083114654	25.347660959054817
26	24.15083376012005	25.33923826339157	26.538636434972613	23.971291541515765
27	25.660310160247306	24.843908048768984	25.57001066086142	23.92577113012229
28	23.862048863306747	25.449947815564418	25.993117048813623	24.694886272315212
29	23.826260293157127	25.27849706301864	26.88641174710525	24.008830896718983
30	24.290186786683247	25.46081530116736	26.627894927029327	23.621102985120064
31	24.984948769032492	24.951967764103955	25.97597197355904	24.08711149330451
32	24.203656995656004	25.17687266571851	25.73089361173234	24.888576726893145
33	23.624699192257175	24.481006584686813	26.33246966528302	25.56182455777299
34	24.882078317505027	25.05551644767921	26.661286025754205	23.40111920906156
35	24.317189885888247	24.73084834368636	27.177688752373218	23.77427301805217
36	23.68222273361269	25.656146130930647	26.458810100231656	24.202821035225007
37	24.47419271853228	25.319774580903857	26.881711441285688	23.324321259278175
38	24.34630654542822	24.292805077844477	26.556838290394673	24.80405008633263
39	24.322079465767658	24.784854542096365	26.400198233032015	24.492867759103966
40	24.68110081162294	24.32424034461759	27.004424123420566	23.9902347203389
41	23.2081700778847	25.713969356214257	26.800922963407174	24.276937602493874
42	24.803040624680108	25.20193570581007	27.002121289025748	22.99290238048408
43	23.62357932073641	24.009146353485395	26.60978770863719	25.757486617141005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1676.0
1	2148.0
2	2620.0
3	4804.5
4	6989.0
5	6989.0
6	8995.5
7	11002.0
8	9406.5
9	7811.0
10	10714.5
11	13618.0
12	13618.0
13	22718.0
14	31818.0
15	42658.0
16	53498.0
17	49386.0
18	45274.0
19	45274.0
20	52995.5
21	60717.0
22	51603.0
23	42489.0
24	49268.0
25	56047.0
26	56047.0
27	65826.5
28	75606.0
29	85838.0
30	96070.0
31	109531.5
32	122993.0
33	122993.0
34	142330.5
35	161668.0
36	187479.5
37	213291.0
38	240249.0
39	267207.0
40	267207.0
41	296994.0
42	326781.0
43	350182.0
44	373583.0
45	423745.5
46	473908.0
47	473908.0
48	548242.0
49	622576.0
50	627220.5
51	631865.0
52	590550.0
53	549235.0
54	549235.0
55	518932.5
56	488630.0
57	459209.5
58	429789.0
59	392345.5
60	354902.0
61	354902.0
62	306680.5
63	258459.0
64	238173.5
65	217888.0
66	186046.5
67	154205.0
68	154205.0
69	118930.5
70	83656.0
71	69814.0
72	55972.0
73	37775.5
74	19579.0
75	19579.0
76	15335.5
77	11092.0
78	9690.5
79	8289.0
80	6198.0
81	4107.0
82	4107.0
83	3306.0
84	2505.0
85	1985.0
86	1465.0
87	1013.5
88	562.0
89	562.0
90	421.0
91	280.0
92	209.5
93	139.0
94	130.5
95	122.0
96	122.0
97	69.0
98	16.0
99	15.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6340013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.6020077512571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.05224813783978	36.96145247838584
2	9.980325669681752	9.10245777097795
3	3.284357695791725	4.493199153041854
4	1.5805491787964425	2.883048636108736
5	0.9379878896163161	2.138706550643427
6	0.6095091511691467	1.6676904621646536
7	0.4194447149960633	1.338926480113201
8	0.31784481539790493	1.1595489388377713
9	0.2372627635540751	0.9737692544409852
>10	1.3703540343628227	11.401345658325683
>50	0.10822563883960841	3.4308953791567944
>100	0.07885351055415948	7.4335838547178925
>500	0.011881063346199297	3.7788818902736896
>1k	0.01029228899139656	9.501892612754139
>5k	5.871440028568928E-4	1.7258814785747736
>10k+	2.763030601679496E-4	2.008719401482656
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27553	0.43458901424965535	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24627	0.38843768932334993	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20315	0.3204252104845842	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11613	0.1831699714180397	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11522	0.18173464313085794	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	10851	0.17115106861768264	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	10538	0.16621417022331025	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	10519	0.16591448629521738	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	9303	0.1467347148972723	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	9219	0.1454097964783353	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	7070	0.11151396692719716	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6878	0.10848558196962688	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	6822	0.10760230302366888	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6821	0.1075865301853482	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6634	0.10463700941938131	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6559	0.10345404654533041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5772838320678522E-5	1.5772838320678522E-5
2	0.0	0.0	0.0	4.731851496203557E-5	1.5772838320678522E-5
3	0.0	0.0	0.0	4.731851496203557E-5	1.5772838320678522E-5
4	0.0	0.0	0.0	9.463702992407114E-5	1.5772838320678522E-5
5	0.0	0.0	0.0	1.7350122152746375E-4	1.5772838320678522E-5
6	0.0	0.0	0.0	1.8927405984814227E-4	3.1545676641357044E-5
7	0.0	0.0	0.0	2.050468981688208E-4	3.1545676641357044E-5
8	0.0	0.0	0.0	2.2081973648949932E-4	3.1545676641357044E-5
9	0.0	0.0	0.0	2.996839280928919E-4	3.1545676641357044E-5
10	0.0	0.0	0.0	3.470024430549275E-4	3.1545676641357044E-5
11	0.0	0.0	0.0	3.470024430549275E-4	3.1545676641357044E-5
12	0.0	0.0	0.0	3.62775281375606E-4	3.1545676641357044E-5
13	0.0	0.0	0.0	3.7854811969628455E-4	3.1545676641357044E-5
14	1.5772838320678522E-5	0.0	0.0	4.258666346583201E-4	4.731851496203557E-5
15	1.5772838320678522E-5	0.0	0.0	4.4163947297899865E-4	4.731851496203557E-5
16	1.5772838320678522E-5	0.0	0.0	4.7318514962035565E-4	6.309135328271409E-5
17	1.5772838320678522E-5	0.0	0.0	5.047308262617127E-4	6.309135328271409E-5
18	1.5772838320678522E-5	0.0	0.0	5.205036645823912E-4	7.886419160339261E-5
19	1.5772838320678522E-5	0.0	0.0	6.466863711478194E-4	7.886419160339261E-5
20	1.5772838320678522E-5	0.0	0.0	8.044147543546046E-4	7.886419160339261E-5
21	1.5772838320678522E-5	0.0	0.0	0.001025234490844104	7.886419160339261E-5
22	1.5772838320678522E-5	0.0	0.0	0.00138800977221971	7.886419160339261E-5
23	1.5772838320678522E-5	0.0	0.0	0.0021293331732916004	1.2618270656542817E-4
24	1.5772838320678522E-5	0.0	0.0	0.002933747927646205	1.2618270656542817E-4
25	1.5772838320678522E-5	0.0	0.0	0.0038170268736042023	1.419555448861067E-4
26	1.5772838320678522E-5	0.0	0.0	0.006135634106743945	1.419555448861067E-4
27	1.5772838320678522E-5	0.0	0.0	0.014069371782045242	1.419555448861067E-4
28	1.5772838320678522E-5	0.0	0.0	0.0339273752277795	1.419555448861067E-4
29	1.5772838320678522E-5	0.0	0.0	0.06063079050468824	1.419555448861067E-4
30	1.5772838320678522E-5	0.0	0.0	0.10091461957570119	1.419555448861067E-4
31	1.5772838320678522E-5	0.0	0.0	0.19061475110539994	1.419555448861067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2480	0.0	17.754032	3
CAAGACG	1655	0.0	16.767372	4
AAGACGG	1680	0.0	16.517857	5
ATCTCCG	2400	0.0	15.879167	10
TACACAT	2775	0.0	15.466666	5
TCTAGCG	1140	0.0	15.254387	28
TTATACA	2690	0.0	15.130112	2
CGCAATA	1140	0.0	14.929826	36
CTAGCGG	1185	0.0	14.8312235	29
ACACATC	2785	0.0	14.813285	6
ACATCTC	2840	0.0	14.786972	8
CCCACGA	2655	0.0	14.354049	19
GCAATCT	2685	0.0	14.262569	34
CGCAAGA	1870	0.0	14.245989	2
ATACACA	3355	0.0	13.950821	4
TACGGAT	3070	0.0	13.859935	27
CACGAGA	2755	0.0	13.833031	21
GGCAATC	2770	0.0	13.824909	33
CCGAGCC	4150	0.0	13.819277	14
GCGCAAG	2035	0.0	13.818182	1
>>END_MODULE