FastQCFastQC Report
Thu 9 Feb 2017
SRR2727472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658008
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT105900.2895018272239973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102170.27930502065605106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101560.277637446391588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75610.20669719694434785No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG66020.1804807425243466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC62960.17211553391900727No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC46760.12782913542015217No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA40850.11167280115297726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.11055197254899389No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38700.10579528530282056No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG38030.10396368734021358No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA37820.10338960439670991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC23650.023.467233
CTTATAC24450.022.0184061
TTATACA25500.021.6196082
TACACAT28300.019.6766785
ATCTCCG28350.019.05467410
CGCTATC27000.018.84259433
CTACGCT27800.018.6996430
CGCTACG26750.018.67289728
GCTACGC27650.018.60036329
TATCTCG26700.018.56928836
GACGCTA27550.018.26497326
ACGCTAC27900.018.03584127
TCTCCGA29850.018.03517511
CGAGACG28550.018.0140123
ATACACA32050.017.951644
ACACATC31000.017.9032256
ACATCTC31750.017.8881878
GCCCACG30500.017.77213118
CACGAGA30200.017.76490221
CCCACGA30500.017.6508219
Produced by FastQC (version 0.11.5)