##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727472.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3658008
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322109465042175	31.0	31.0	34.0	30.0	34.0
2	31.606882762421513	31.0	31.0	34.0	30.0	34.0
3	31.720462065692583	31.0	31.0	34.0	30.0	34.0
4	35.490051415961915	37.0	35.0	37.0	33.0	37.0
5	35.29092090558577	37.0	35.0	37.0	33.0	37.0
6	35.290333700746416	37.0	35.0	37.0	32.0	37.0
7	35.2089016754474	37.0	35.0	37.0	32.0	37.0
8	35.19756517755019	37.0	35.0	37.0	32.0	37.0
9	36.8311887234801	39.0	37.0	39.0	32.0	39.0
10	36.604989655572105	39.0	35.0	39.0	32.0	39.0
11	36.722752930009996	39.0	35.0	39.0	32.0	39.0
12	36.55439080505018	39.0	35.0	39.0	32.0	39.0
13	36.66330008026226	39.0	35.0	39.0	32.0	39.0
14	37.77447452274571	40.0	37.0	41.0	32.0	41.0
15	37.72466025224658	40.0	37.0	41.0	32.0	41.0
16	37.69308104301576	39.0	37.0	41.0	32.0	41.0
17	37.64361340926537	39.0	36.0	41.0	32.0	41.0
18	37.62083461818563	39.0	36.0	41.0	32.0	41.0
19	37.62663969023578	39.0	37.0	41.0	32.0	41.0
20	37.595991588864756	39.0	36.0	41.0	32.0	41.0
21	37.53092912864051	39.0	36.0	41.0	32.0	41.0
22	37.45460890189415	39.0	36.0	41.0	32.0	41.0
23	37.36026192397611	39.0	36.0	41.0	31.0	41.0
24	37.27992913082749	39.0	36.0	41.0	31.0	41.0
25	37.19506490964481	39.0	36.0	41.0	31.0	41.0
26	36.99482040498545	39.0	36.0	41.0	31.0	41.0
27	36.92257452690098	39.0	36.0	40.0	30.0	41.0
28	36.76583457444598	39.0	36.0	40.0	30.0	41.0
29	36.62567222378956	39.0	35.0	40.0	30.0	41.0
30	36.76133157718627	39.0	35.0	40.0	30.0	41.0
31	36.764788103251824	39.0	35.0	40.0	30.0	41.0
32	36.737921841614344	39.0	35.0	40.0	30.0	41.0
33	36.63684497136146	39.0	35.0	40.0	30.0	41.0
34	36.59651509783467	39.0	35.0	40.0	30.0	41.0
35	36.51247564248082	39.0	35.0	40.0	30.0	41.0
36	36.43229839847261	39.0	35.0	40.0	30.0	41.0
37	36.354390969073876	38.0	35.0	40.0	30.0	41.0
38	36.23772966051468	38.0	35.0	40.0	29.0	41.0
39	36.12332203756799	38.0	35.0	40.0	29.0	41.0
40	35.99955139518558	38.0	35.0	40.0	28.0	41.0
41	35.87387124358394	38.0	34.0	40.0	28.0	41.0
42	35.74862985537484	38.0	34.0	40.0	27.0	41.0
43	35.582560234969414	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	6.0
16	19.0
17	53.0
18	149.0
19	397.0
20	853.0
21	1841.0
22	3551.0
23	6547.0
24	10930.0
25	17451.0
26	26607.0
27	38330.0
28	53509.0
29	72908.0
30	95129.0
31	121736.0
32	150020.0
33	183554.0
34	227347.0
35	281544.0
36	353195.0
37	461828.0
38	673339.0
39	877163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07847549813997	19.562067660868976	12.122690819702964	26.236766021288087
2	18.538860494564254	21.93390501059593	33.826333895387876	25.70090059945194
3	20.349682122073	24.04376917710404	29.554035967116526	26.052512733706436
4	14.684549623729637	16.551713391550813	33.93603841216312	34.82769857255643
5	14.537721076607815	36.74149974521652	33.93603841216312	14.784740766012542
6	34.23937290459725	34.83248259708563	15.993978143295475	14.934166355021642
7	28.273584967556115	30.769287546664742	21.455666581374345	19.5014609044048
8	27.623449702679707	33.14560821080763	20.165565520906462	19.065376565606197
9	26.664922548009734	14.124682067398432	19.489323150742152	39.72107223384968
10	17.39804833669035	26.725611316322983	32.52398573212524	23.352354614861422
11	36.27523504595944	21.76493326422468	20.99626354015628	20.963568149659594
12	21.22485790080284	25.89010193526094	29.328995453263083	23.55604471067313
13	30.32776308854437	19.608650391141847	25.1427006173852	24.92088590292859
14	22.62277720551732	20.987734307852797	24.66156443616307	31.72792405046681
15	25.651884850989937	27.344172019306683	21.985381114530096	25.018562015173284
16	25.452705406877186	25.574602351881133	24.634063129440943	24.33862911180074
17	24.074796993336264	26.659482428687966	24.961673129200374	24.3040474487754
18	24.89158033552688	24.181767781809114	26.319816687114955	24.606835195549053
19	24.25464897835106	25.873043470653972	25.855356248537454	24.016951302457514
20	24.289531351489664	24.432778714535342	26.088734633713212	25.188955300261785
21	25.084062145298752	25.05330770189677	25.911370341453598	23.951259811350877
22	24.92211608066467	25.3022683383962	25.692316692582413	24.083298888356726
23	24.01941165792967	24.68400834552576	26.084305993863328	25.21227400268124
24	24.158613103087802	25.988516154147284	25.852485833819937	24.000384908944977
25	24.49456097416955	25.084636228242257	25.319244791154095	25.101558006434104
26	24.686714736545138	25.597100935809873	25.91027685013264	23.80590747751235
27	25.169737190295923	25.076052321372728	25.36194562723756	24.3922648610938
28	24.079526343299413	24.982968872676057	26.480477899446914	24.45702688457762
29	24.28277904258274	25.078211966731622	26.37432722946478	24.26468176122086
30	23.47652055435636	25.80152913826323	26.58036833161655	24.14158197576386
31	24.635402656309115	25.120776116399963	25.761589367765186	24.48223185952573
32	23.835623104159424	25.55573962659458	25.674328760352626	24.934308508893366
33	23.55410376357843	25.100819899792455	26.135043991155843	25.210032345473277
34	24.886030867073007	25.186905004034983	25.997346096564034	23.929718032327976
35	24.29144496130134	25.12763777443898	26.798164465468638	23.782752798791034
36	23.692184380132574	25.617685909926934	26.218477378945042	24.47165233099545
37	24.471488307297303	25.4906768929975	26.17954908791889	23.858285711786305
38	23.696558345416413	24.802214757321472	26.750925640403196	24.750301256858926
39	24.535156839460164	24.533516602478723	25.896717557752748	25.034609000308368
40	24.044561958311736	24.85090245838719	27.109426769979727	23.995108813321348
41	23.469850257298507	25.327063254098952	26.719733800472827	24.483352688129713
42	24.704347284095608	25.117140257757775	26.801526951280586	23.37698550686603
43	23.857656954276756	23.99628431649138	26.909755254772545	25.23630347445932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	403.0
2	457.0
3	1146.5
4	1836.0
5	1836.0
6	2598.0
7	3360.0
8	3033.0
9	2706.0
10	3641.5
11	4577.0
12	4577.0
13	8126.5
14	11676.0
15	17510.0
16	23344.0
17	21919.5
18	20495.0
19	20495.0
20	24247.5
21	28000.0
22	26175.5
23	24351.0
24	29797.5
25	35244.0
26	35244.0
27	42424.5
28	49605.0
29	56473.0
30	63341.0
31	72815.5
32	82290.0
33	82290.0
34	95026.5
35	107763.0
36	124109.5
37	140456.0
38	156940.5
39	173425.0
40	173425.0
41	188442.0
42	203459.0
43	215515.5
44	227572.0
45	249073.5
46	270575.0
47	270575.0
48	304048.5
49	337522.0
50	334611.0
51	331700.0
52	317460.5
53	303221.0
54	303221.0
55	286057.0
56	268893.0
57	259527.0
58	250161.0
59	232142.0
60	214123.0
61	214123.0
62	185167.0
63	156211.0
64	140986.0
65	125761.0
66	106539.5
67	87318.0
68	87318.0
69	68315.0
70	49312.0
71	40537.5
72	31763.0
73	22039.5
74	12316.0
75	12316.0
76	9401.5
77	6487.0
78	5443.5
79	4400.0
80	3276.0
81	2152.0
82	2152.0
83	1549.0
84	946.0
85	723.5
86	501.0
87	363.0
88	225.0
89	225.0
90	143.5
91	62.0
92	43.0
93	24.0
94	23.5
95	23.0
96	23.0
97	13.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3658008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.62285322407388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43377867136783	47.808814568628
2	9.024448981776157	10.219801002465084
3	2.673689778163615	4.54175831626995
4	1.2003722205668845	2.718740002376573
5	0.6621931266099587	1.8747632107013128
6	0.4221397861913474	1.4341655492132754
7	0.28112867230257416	1.1142815284207193
8	0.20115730968192597	0.9112080656855416
9	0.1488206401117567	0.7583984335584647
>10	0.8187989195535603	8.425559478812765
>50	0.06586660806729178	2.5972748900126534
>100	0.0519756434122526	6.223376800143194
>500	0.008944964462018954	3.615735311261016
>1k	0.006396130492614737	6.355870240717511
>5k	1.44273620134167E-4	0.557111119343468
>10k+	1.44273620134167E-4	0.8431414823907231
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT	10590	0.2895018272239973	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10217	0.27930502065605106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10156	0.277637446391588	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7561	0.20669719694434785	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6602	0.1804807425243466	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6296	0.17211553391900727	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	4676	0.12782913542015217	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	4085	0.11167280115297726	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4044	0.11055197254899389	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3870	0.10579528530282056	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	3803	0.10396368734021358	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3782	0.10338960439670991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0934913209593856E-4
2	0.0	5.467456604796928E-5	0.0	0.0	1.0934913209593856E-4
3	0.0	5.467456604796928E-5	0.0	0.0	1.0934913209593856E-4
4	0.0	8.201184907195391E-5	0.0	0.0	1.0934913209593856E-4
5	0.0	8.201184907195391E-5	0.0	2.733728302398464E-5	1.0934913209593856E-4
6	0.0	8.201184907195391E-5	0.0	2.733728302398464E-5	1.366864151199232E-4
7	0.0	8.201184907195391E-5	0.0	2.733728302398464E-5	1.366864151199232E-4
8	0.0	1.0934913209593856E-4	0.0	2.733728302398464E-5	1.366864151199232E-4
9	0.0	2.733728302398464E-4	0.0	2.733728302398464E-5	1.6402369814390782E-4
10	0.0	3.00710113263831E-4	0.0	2.733728302398464E-5	1.6402369814390782E-4
11	0.0	3.00710113263831E-4	0.0	5.467456604796928E-5	1.6402369814390782E-4
12	0.0	3.00710113263831E-4	0.0	5.467456604796928E-5	1.6402369814390782E-4
13	0.0	3.00710113263831E-4	0.0	5.467456604796928E-5	1.6402369814390782E-4
14	0.0	3.00710113263831E-4	0.0	8.201184907195391E-5	1.9136098116789247E-4
15	0.0	3.00710113263831E-4	0.0	8.201184907195391E-5	2.1869826419187711E-4
16	0.0	3.00710113263831E-4	0.0	1.6402369814390782E-4	2.4603554721586173E-4
17	0.0	3.00710113263831E-4	0.0	3.00710113263831E-4	2.4603554721586173E-4
18	0.0	3.2804739628781564E-4	0.0	3.553846793118003E-4	2.733728302398464E-4
19	0.0	3.553846793118003E-4	0.0	4.920710944317235E-4	3.00710113263831E-4
20	0.0	3.553846793118003E-4	0.0	5.467456604796928E-4	3.00710113263831E-4
21	0.0	3.553846793118003E-4	0.0	6.83432075599616E-4	3.00710113263831E-4
22	0.0	3.553846793118003E-4	0.0	9.568049058394624E-4	3.553846793118003E-4
23	0.0	3.553846793118003E-4	0.0	0.0013942014342232165	3.553846793118003E-4
24	0.0	3.8272196233578494E-4	0.0	0.0021596453588947863	3.553846793118003E-4
25	0.0	3.8272196233578494E-4	0.0	0.002870414717518387	4.1005924535976956E-4
26	0.0	3.8272196233578494E-4	0.0	0.005576805736892866	4.1005924535976956E-4
27	0.0	3.8272196233578494E-4	0.0	0.01320390770058458	4.1005924535976956E-4
28	0.0	3.8272196233578494E-4	0.0	0.031082490798270534	4.1005924535976956E-4
29	0.0	3.8272196233578494E-4	0.0	0.05544000997264085	4.3739652838375423E-4
30	0.0	3.8272196233578494E-4	0.0	0.09045906952636516	4.3739652838375423E-4
31	0.0	3.8272196233578494E-4	0.0	0.16101659701126952	4.3739652838375423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2365	0.0	23.46723	3
CTTATAC	2445	0.0	22.018406	1
TTATACA	2550	0.0	21.619608	2
TACACAT	2830	0.0	19.676678	5
ATCTCCG	2835	0.0	19.054674	10
CGCTATC	2700	0.0	18.842594	33
CTACGCT	2780	0.0	18.69964	30
CGCTACG	2675	0.0	18.672897	28
GCTACGC	2765	0.0	18.600363	29
TATCTCG	2670	0.0	18.569288	36
GACGCTA	2755	0.0	18.264973	26
ACGCTAC	2790	0.0	18.035841	27
TCTCCGA	2985	0.0	18.035175	11
CGAGACG	2855	0.0	18.01401	23
ATACACA	3205	0.0	17.95164	4
ACACATC	3100	0.0	17.903225	6
ACATCTC	3175	0.0	17.888187	8
GCCCACG	3050	0.0	17.772131	18
CACGAGA	3020	0.0	17.764902	21
CCCACGA	3050	0.0	17.65082	19
>>END_MODULE