FastQCFastQC Report
Thu 9 Feb 2017
SRR2727471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5886054
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185520.315185691466643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179710.30531490196997857No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG154700.26282463599552436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC154040.2617033414916003No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT137370.23338216061218603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134750.22893096121782097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC98090.16664814831804126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA93060.15810252505328695No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA84860.1441712903075643No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG80220.1362882501587651No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG79470.13501405185885146No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT74550.12665531101141783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73040.12408992510092501No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG64160.10900341722994726No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC63390.10769524030870258No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA62750.10660792442610958No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC60860.10339694471032715No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG60340.10251350055572035No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC59610.10127328087713773No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC58880.1000330611985551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC24650.023.4908713
TTATACA28750.019.9478262
TACACAT30000.019.3016665
CTTATAC29550.019.0321481
ATACACA32450.018.0724184
ACACATC33900.017.299416
ATCTCCG33800.017.13165710
AAGACGG19750.016.767095
GCCGTCT9300.016.31182736
ACATCTC36550.016.2982228
CAAGACG21450.015.7832174
CGCTTCG9350.015.63101732
CTAGCGG11850.015.29957929
CACGAGA36350.015.26822721
CGCAAGA21850.015.2402742
TCTAGCG11900.015.07983228
CACATCT39150.015.0268197
CGAGACC37900.014.93667523
TCTCCGA38850.014.85714211
CCCACGA38450.014.771131519
Produced by FastQC (version 0.11.5)