##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5886054
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288672852814464	31.0	31.0	34.0	30.0	34.0
2	31.566920045245933	31.0	31.0	34.0	30.0	34.0
3	31.67739541635194	31.0	31.0	34.0	30.0	34.0
4	35.45338761757877	37.0	35.0	37.0	33.0	37.0
5	35.25682961794099	37.0	35.0	37.0	33.0	37.0
6	35.262278939336944	37.0	35.0	37.0	32.0	37.0
7	35.17431780272488	37.0	35.0	37.0	32.0	37.0
8	35.16612470765644	37.0	35.0	37.0	32.0	37.0
9	36.79172277386514	39.0	37.0	39.0	32.0	39.0
10	36.56622382329486	39.0	35.0	39.0	32.0	39.0
11	36.68308037948683	39.0	35.0	39.0	32.0	39.0
12	36.51281826500402	39.0	35.0	39.0	32.0	39.0
13	36.61800180562394	39.0	35.0	39.0	32.0	39.0
14	37.716573446319046	40.0	37.0	41.0	32.0	41.0
15	37.66479274570026	39.0	36.0	41.0	32.0	41.0
16	37.618959323173044	39.0	36.0	41.0	32.0	41.0
17	37.5780315641005	39.0	36.0	41.0	32.0	41.0
18	37.55586238250618	39.0	36.0	41.0	32.0	41.0
19	37.54733272919345	39.0	36.0	41.0	32.0	41.0
20	37.52948681748418	39.0	36.0	41.0	32.0	41.0
21	37.462697589930364	39.0	36.0	41.0	32.0	41.0
22	37.384259981304965	39.0	36.0	41.0	31.0	41.0
23	37.295587672148436	39.0	36.0	41.0	31.0	41.0
24	37.20561483126047	39.0	36.0	41.0	31.0	41.0
25	37.11527094382756	39.0	36.0	41.0	31.0	41.0
26	36.914172550914415	39.0	36.0	40.0	30.0	41.0
27	36.820715712088266	39.0	36.0	40.0	30.0	41.0
28	36.659348011418174	39.0	35.0	40.0	30.0	41.0
29	36.5264701954824	39.0	35.0	40.0	30.0	41.0
30	36.64752005333285	39.0	35.0	40.0	30.0	41.0
31	36.65693230133465	39.0	35.0	40.0	30.0	41.0
32	36.615311888066266	39.0	35.0	40.0	30.0	41.0
33	36.50209631104302	39.0	35.0	40.0	30.0	41.0
34	36.46590992879101	39.0	35.0	40.0	30.0	41.0
35	36.36930751909514	38.0	35.0	40.0	30.0	41.0
36	36.293666860684596	38.0	35.0	40.0	30.0	41.0
37	36.20062524061111	38.0	35.0	40.0	29.0	41.0
38	36.081674921772716	38.0	35.0	40.0	29.0	41.0
39	35.96227608513276	38.0	35.0	40.0	28.0	41.0
40	35.834306311155146	38.0	34.0	40.0	28.0	41.0
41	35.69042808645656	38.0	34.0	40.0	27.0	41.0
42	35.56517388389573	38.0	34.0	40.0	27.0	41.0
43	35.39160089255043	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	9.0
15	13.0
16	47.0
17	143.0
18	311.0
19	757.0
20	1689.0
21	3539.0
22	6521.0
23	11443.0
24	19253.0
25	30117.0
26	45169.0
27	64662.0
28	90861.0
29	121714.0
30	158167.0
31	199721.0
32	247416.0
33	303049.0
34	372300.0
35	458908.0
36	575032.0
37	748700.0
38	1082523.0
39	1343981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69806223320411	19.07287972553429	12.14910702484211	26.07995101641949
2	19.017052850687406	21.91172558049926	32.72523493668254	26.345986632130796
3	20.739802930792003	23.77241187389718	29.220068317416047	26.267716877894763
4	15.062688857424686	16.143310951615465	33.070338804231156	35.7236613867287
5	14.76914754774591	36.69414177987494	33.335287104059866	15.201423568319287
6	35.03095622296364	33.9255297352012	15.73109930693806	15.312414734897098
7	28.812053032473028	30.740254846455706	20.905533656334107	19.542158464737156
8	28.553628627939876	32.13310649205733	20.001617382375354	19.311647497627444
9	27.155170509818632	13.88747028144832	19.047174898497364	39.910184310235685
10	18.053555064224692	26.19534241445967	31.68080007420931	24.07030244710633
11	36.721919302813056	21.404560678512293	20.332823993799582	21.540696024875068
12	21.745485175637192	25.72106881792114	28.537471793496966	23.9959742129447
13	30.665875644362078	18.95137217565452	24.880862458958074	25.50188972102532
14	23.169818013902013	20.739378198025367	24.062623278685518	32.028180509387106
15	26.41416473583151	26.72636030862102	21.615465981114003	25.24400897443347
16	25.972799434052085	24.934990402738404	24.384910502010346	24.707299661199166
17	24.822317294404705	26.182107741451233	24.36019105499202	24.63538390915204
18	25.607631190607492	23.23373180062568	25.92030246409564	25.238334544671183
19	24.73293653099343	25.48811139007559	25.336413835143208	24.442538243787773
20	24.76574288988854	23.730601180349346	25.61882714633607	25.884828783426045
21	25.531264239166003	24.600351950559745	25.56682286638893	24.301560943885324
22	25.632350637625816	24.631544324941636	25.068373480773364	24.66773155665918
23	24.432939283261756	24.016310417811322	25.723362374860987	25.82738792406594
24	24.33018793235672	25.672224549757782	25.426083416835795	24.5715041010497
25	25.224539224410787	24.341383888085293	24.698482208963764	25.735594678540156
26	25.109147826370602	25.1682706274866	25.36791201711707	24.354669529025728
27	26.052411343830688	24.414420934636347	24.78611307337649	24.74705464815647
28	24.625428173102048	24.487950671196696	26.029135988218933	24.857485167482324
29	24.398586897096088	24.40876349418473	26.02047143977952	25.17217816893967
30	23.96410226613619	25.661963006115812	26.39802489069927	23.975909837048725
31	25.55749573483356	24.69197530297887	24.68460194215004	25.06592702003753
32	24.52865705955127	24.86258196068198	25.103966086617625	25.50479489314913
33	24.26547564803177	24.48429796940361	25.66046794677725	25.589758435787367
34	25.102776834871037	24.972723661726516	25.464037536862556	24.460461966539892
35	24.94766442849488	24.641534039612957	26.58856000981303	23.82224152207914
36	24.503529869076974	25.2341381849368	25.323637873522735	24.938694072463488
37	25.165399433984128	24.890971098804055	25.709448129425926	24.23418133778589
38	24.267361461515645	24.24840139081293	26.09233622389465	25.391900923776777
39	25.253590945648817	23.90819044473598	25.246897157246607	25.591321452368597
40	24.638323059897175	24.301917719409303	26.557877314751106	24.50188190594242
41	23.80863308423606	25.001282692955247	26.139192742710144	25.050891480098553
42	25.452484805610005	24.69270584333749	26.38582656564143	23.468982785411075
43	24.501559109039775	23.259419638351943	26.25008197342396	25.988939279184326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	658.0
1	816.0
2	974.0
3	2214.0
4	3454.0
5	3454.0
6	4822.5
7	6191.0
8	5572.0
9	4953.0
10	6602.0
11	8251.0
12	8251.0
13	14274.5
14	20298.0
15	29777.0
16	39256.0
17	35736.5
18	32217.0
19	32217.0
20	37541.0
21	42865.0
22	37077.0
23	31289.0
24	37034.5
25	42780.0
26	42780.0
27	51254.0
28	59728.0
29	68388.5
30	77049.0
31	88122.5
32	99196.0
33	99196.0
34	116886.0
35	134576.0
36	160019.0
37	185462.0
38	212593.5
39	239725.0
40	239725.0
41	269164.0
42	298603.0
43	320665.0
44	342727.0
45	388266.5
46	433806.0
47	433806.0
48	509972.5
49	586139.0
50	581293.0
51	576447.0
52	544124.0
53	511801.0
54	511801.0
55	484566.5
56	457332.0
57	440851.5
58	424371.0
59	397130.0
60	369889.0
61	369889.0
62	321139.0
63	272389.0
64	251610.0
65	230831.0
66	195476.5
67	160122.0
68	160122.0
69	124149.5
70	88177.0
71	72667.5
72	57158.0
73	38885.5
74	20613.0
75	20613.0
76	15877.5
77	11142.0
78	9547.0
79	7952.0
80	5862.5
81	3773.0
82	3773.0
83	2959.5
84	2146.0
85	1588.5
86	1031.0
87	718.0
88	405.0
89	405.0
90	278.5
91	152.0
92	108.0
93	64.0
94	56.0
95	48.0
96	48.0
97	28.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5886054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.18895688082422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09603238980112	34.16075132327398
2	10.869278102008284	9.388655665466455
3	3.577885823468712	4.635754696629075
4	1.7498021257446785	3.02288514235046
5	1.0280828054743936	2.2200911977775193
6	0.7050325556739679	1.8269772387948184
7	0.49074197077270054	1.4836243670719003
8	0.3506394706172723	1.2115002381763527
9	0.2921528692560545	1.1355999905617935
>10	1.625384838130882	12.885374726998544
>50	0.11412533476555453	3.368103006183873
>100	0.07554456154110266	6.5787394609066645
>500	0.012491456185799124	3.786476479979594
>1k	0.011745102059375186	10.34789099370101
>5k	8.249068336000485E-4	2.3425173131309016
>10k+	2.3568766674287095E-4	1.6050581589970994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18552	0.315185691466643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17971	0.30531490196997857	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	15470	0.26282463599552436	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15404	0.2617033414916003	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	13737	0.23338216061218603	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13475	0.22893096121782097	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	9809	0.16664814831804126	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	9306	0.15810252505328695	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	8486	0.1441712903075643	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	8022	0.1362882501587651	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7947	0.13501405185885146	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7455	0.12665531101141783	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7304	0.12408992510092501	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	6416	0.10900341722994726	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6339	0.10769524030870258	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	6275	0.10660792442610958	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6086	0.10339694471032715	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	6034	0.10251350055572035	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	5961	0.10127328087713773	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	5888	0.1000330611985551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.397862133103094E-5	6.795724266206189E-5
2	0.0	0.0	0.0	3.397862133103094E-5	6.795724266206189E-5
3	0.0	0.0	0.0	5.0967931996546414E-5	6.795724266206189E-5
4	0.0	0.0	0.0	5.0967931996546414E-5	6.795724266206189E-5
5	0.0	0.0	0.0	1.0193586399309283E-4	6.795724266206189E-5
6	0.0	0.0	0.0	1.0193586399309283E-4	6.795724266206189E-5
7	0.0	0.0	0.0	1.189251746586083E-4	6.795724266206189E-5
8	0.0	0.0	0.0	1.189251746586083E-4	6.795724266206189E-5
9	0.0	0.0	0.0	1.5290379598963924E-4	1.3591448532412377E-4
10	0.0	0.0	0.0	1.5290379598963924E-4	1.3591448532412377E-4
11	0.0	0.0	0.0	1.6989310665515471E-4	1.3591448532412377E-4
12	0.0	0.0	0.0	1.8688241732067019E-4	1.8688241732067019E-4
13	0.0	0.0	0.0	2.0387172798618566E-4	2.0387172798618566E-4
14	0.0	0.0	0.0	2.378503493172166E-4	2.378503493172166E-4
15	0.0	1.698931066551547E-5	0.0	2.5483965998273207E-4	2.88818281313763E-4
16	0.0	1.698931066551547E-5	0.0	3.9075414530685584E-4	3.3978621331030943E-4
17	0.0	1.698931066551547E-5	0.0	4.926900092999487E-4	3.3978621331030943E-4
18	0.0	1.698931066551547E-5	0.0	5.266686306309796E-4	4.5871138796891773E-4
19	0.0	1.698931066551547E-5	0.0	6.11615183958557E-4	4.5871138796891773E-4
20	0.0	1.698931066551547E-5	0.0	8.66454843941289E-4	5.606472519620106E-4
21	0.0	1.698931066551547E-5	0.0	0.0012402196785826294	5.606472519620106E-4
22	0.0	1.698931066551547E-5	0.0	0.001512048649230877	6.11615183958557E-4
23	0.0	1.698931066551547E-5	0.0	0.0019028027945377328	6.795724266206189E-4
24	0.0	1.698931066551547E-5	0.0	0.0028202255704755682	6.965617372861343E-4
25	0.0	1.698931066551547E-5	0.0	0.003652701793085826	6.965617372861343E-4
26	0.0	1.698931066551547E-5	0.0	0.005674429762282167	7.135510479516498E-4
27	0.0	1.698931066551547E-5	0.0	0.012775961620467634	7.475296692826807E-4
28	0.0	1.698931066551547E-5	0.0	0.029153657102024548	7.475296692826807E-4
29	0.0	1.698931066551547E-5	0.0	0.0509509426858809	7.475296692826807E-4
30	0.0	1.698931066551547E-5	0.0	0.08304375053303963	7.475296692826807E-4
31	0.0	1.698931066551547E-5	0.0	0.1475011951980053	7.645189799481962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2465	0.0	23.490871	3
TTATACA	2875	0.0	19.947826	2
TACACAT	3000	0.0	19.301666	5
CTTATAC	2955	0.0	19.032148	1
ATACACA	3245	0.0	18.072418	4
ACACATC	3390	0.0	17.29941	6
ATCTCCG	3380	0.0	17.131657	10
AAGACGG	1975	0.0	16.76709	5
GCCGTCT	930	0.0	16.311827	36
ACATCTC	3655	0.0	16.298222	8
CAAGACG	2145	0.0	15.783217	4
CGCTTCG	935	0.0	15.631017	32
CTAGCGG	1185	0.0	15.299579	29
CACGAGA	3635	0.0	15.268227	21
CGCAAGA	2185	0.0	15.240274	2
TCTAGCG	1190	0.0	15.079832	28
CACATCT	3915	0.0	15.026819	7
CGAGACC	3790	0.0	14.936675	23
TCTCCGA	3885	0.0	14.857142	11
CCCACGA	3845	0.0	14.7711315	19
>>END_MODULE