FastQCFastQC Report
Thu 9 Feb 2017
SRR2727470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360075
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT256941.8891605242357958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247371.8187967575317536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238331.7523298347517602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159131.1700090068562394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94200.6926088634817933No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG87070.6401852838997849No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG83130.611216293219124No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT80210.5897468889583295RNA PCR Primer, Index 39 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC79720.5861441464625113No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC67340.4951197544253074No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA53230.39137547561715347No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC52930.389169714905428RNA PCR Primer, Index 39 (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48290.35505394923074096No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.2572652243442457No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT33790.2484421814973439No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33600.2470451997132511No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG32120.23616344686873883No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC26950.1981508372700035No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26560.19528334834476038No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA26270.19315111299009247No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC25860.19013657335073433No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25310.186092678712571No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG24450.1797694980056247No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.17947539657739464No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.178813668363877No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20920.15381504696432183No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC19880.14616840983034024No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA19330.1421245151921769No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18970.13947760233810635No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC18240.13411025127290774No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17730.13036045806297447No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT17190.12639008878186864No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16820.12366965057074059No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC16430.12080216164549748No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG16220.11925812914728967No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC16190.11903755307611712RNA PCR Primer, Index 39 (95% over 24bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15230.11197911879859566No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.1102880355862728No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT14800.10881752844512252No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14200.1044060070216716No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.10249434773817621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC31700.032.681393
TTATACA32150.032.396582
CTTATAC32550.031.8279571
GCCGTCT11300.025.53982436
TACACAT41550.025.2009625
ATACACA41400.025.1135274
ACGTTTA551.9029918E-523.54545426
TACCGAC551.9029918E-523.54545433
CCGTCTT12800.022.69140637
CGAATAC502.7024548E-422.234
ATACCGA502.7024548E-422.232
GTACGAA502.7024548E-422.231
ACACATC49700.021.5150896
TGCGACG705.1030584E-621.14285722
ACGAATA450.003826163220.55555533
CACATCT53000.020.357
GCGCATA656.903318E-519.92307726
AACACAT1600.019.656255
TAGGTCG851.2456512E-619.58823621
ATCGTCA851.2456512E-619.58823610
Produced by FastQC (version 0.11.5)