##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360075
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055213131628772	31.0	31.0	33.0	30.0	34.0
2	31.306827932283145	31.0	31.0	34.0	28.0	34.0
3	31.371250850136942	31.0	31.0	34.0	28.0	34.0
4	35.195337021855416	35.0	35.0	37.0	33.0	37.0
5	35.01546605885705	35.0	35.0	37.0	32.0	37.0
6	35.07915519364741	37.0	35.0	37.0	32.0	37.0
7	34.94864253809533	36.0	35.0	37.0	32.0	37.0
8	35.00580997371468	37.0	35.0	37.0	32.0	37.0
9	36.531548627833025	39.0	35.0	39.0	32.0	39.0
10	36.30670514493686	38.0	35.0	39.0	32.0	39.0
11	36.44108670477731	38.0	35.0	39.0	32.0	39.0
12	36.26739407753249	38.0	35.0	39.0	32.0	39.0
13	36.391892358877264	38.0	35.0	39.0	32.0	39.0
14	37.298159292686066	39.0	36.0	41.0	32.0	41.0
15	37.26001066117677	39.0	36.0	41.0	31.0	41.0
16	37.10986379427605	39.0	36.0	41.0	31.0	41.0
17	37.22161719022848	39.0	36.0	41.0	31.0	41.0
18	37.26156719298568	39.0	36.0	41.0	31.0	41.0
19	37.30015403562304	39.0	36.0	41.0	31.0	41.0
20	37.35256658640149	39.0	36.0	41.0	31.0	41.0
21	37.304829513078325	39.0	36.0	41.0	31.0	41.0
22	37.22126059224675	39.0	36.0	41.0	31.0	41.0
23	37.137254195540685	39.0	36.0	41.0	31.0	41.0
24	37.02586254434498	39.0	36.0	40.0	31.0	41.0
25	36.905948569012736	39.0	35.0	40.0	31.0	41.0
26	36.6394926750363	39.0	35.0	40.0	30.0	41.0
27	36.46978585739757	38.0	35.0	40.0	30.0	41.0
28	36.34158189805709	38.0	35.0	40.0	30.0	41.0
29	36.145341984817016	38.0	35.0	40.0	30.0	41.0
30	36.08113596676654	38.0	35.0	40.0	30.0	41.0
31	36.00038674337812	38.0	35.0	40.0	30.0	41.0
32	35.785280958770656	38.0	34.0	40.0	29.0	41.0
33	35.62733305148613	38.0	34.0	40.0	29.0	41.0
34	35.53862691395695	38.0	34.0	40.0	28.0	41.0
35	35.38055695457971	38.0	34.0	40.0	27.0	41.0
36	35.23383857507858	38.0	34.0	40.0	26.0	41.0
37	35.03213719831627	38.0	33.0	40.0	25.0	41.0
38	34.84236972225796	38.0	33.0	40.0	25.0	41.0
39	34.57984743488411	37.0	33.0	40.0	24.0	41.0
40	34.29807620903259	37.0	33.0	40.0	23.0	41.0
41	34.05751521055824	37.0	33.0	40.0	22.0	41.0
42	33.75071154164292	37.0	33.0	40.0	21.0	41.0
43	33.35417090969248	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	1.0
15	9.0
16	16.0
17	55.0
18	126.0
19	278.0
20	677.0
21	1286.0
22	2313.0
23	3873.0
24	6393.0
25	9959.0
26	14568.0
27	20554.0
28	27785.0
29	36031.0
30	44836.0
31	54510.0
32	66100.0
33	79350.0
34	95905.0
35	115000.0
36	142272.0
37	185740.0
38	227149.0
39	225286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42957189860853	20.033748138889397	13.53182728893627	24.004852673565797
2	17.351984265573588	25.11846773155892	32.390419645975406	25.139128356892083
3	21.311471793834897	26.43890961895484	32.12043453486021	20.129184052350055
4	14.765141628219034	19.050272962888076	29.745418451188353	36.439166957704536
5	12.69687333419113	39.18651544951565	33.11346800727901	15.003143209014208
6	31.65060750326269	33.45881660937816	16.1237431759278	18.766832711431356
7	23.769865632409978	33.566751833538596	20.840541881881514	21.822840652169916
8	29.954303990588755	31.43039905887543	20.941639247835596	17.67365770270022
9	26.27458044593129	12.667242615296951	20.068819734205835	40.98935720456593
10	19.282318989761592	29.50763744646435	29.090895722662353	22.1191478411117
11	32.22726687866478	22.541698068121242	20.389904968476003	24.841130084737976
12	20.144550851975072	29.83306067680091	26.986379427605094	23.036009043618918
13	31.461426759553703	18.242596915611273	27.159605168832602	23.13637115600243
14	22.749627777879898	22.808006911383565	26.126868003602745	28.315497307133796
15	27.43267834494421	25.153098174733014	23.58164071834274	23.832582761980035
16	24.6290829549841	24.073892983842804	28.00360274249582	23.29342131867728
17	21.915482602062387	29.766667279377973	26.55280039703693	21.76504972152271
18	23.896255721191846	21.189419701119423	29.95974486701101	24.95457971067772
19	21.388452842674116	26.187526423175196	30.685293090454568	21.73872764369612
20	21.295810892781645	22.445747477161184	31.63038803007187	24.628053599985293
21	22.52633126849622	24.1183758248626	30.628531514806166	22.72676139183501
22	22.262669338087974	26.88131169236991	29.899601124937963	20.956417844604157
23	20.76936933624984	23.546495597669246	30.38347149973347	25.300663566347442
24	21.921658732055217	26.75543628108744	30.97152730547948	20.351377681377866
25	21.083543186956604	26.2027461720861	28.99575391062993	23.717956730327373
26	22.672499678326563	24.828410197967024	30.4884657096116	22.010624414094814
27	21.584618495303566	26.605076925904818	30.394059151149754	21.416245427641858
28	19.844640920537472	24.699961399187544	31.511644578423986	23.943753101851
29	20.31277686892267	24.227193353307722	32.01338161498447	23.44664816278514
30	19.08806499641564	26.63529584765546	34.19818759994853	20.07845155598037
31	21.64027719059611	25.020679006672424	29.10192452622098	24.237119276510484
32	19.270187305847102	26.76050953072441	32.476003161590356	21.493300001838133
33	19.454735952061466	25.526754039299306	30.990276271529144	24.028233737110085
34	20.01896954212084	26.921530062680365	31.732367700310643	21.32713269488815
35	19.159605168832602	28.476003161590352	32.93899233498153	19.425399334595518
36	20.48585555943606	26.79447824568498	28.69959377240226	24.0200724224767
37	20.19822436262706	28.51460397404555	30.584636876642833	20.702534786684556
38	20.417035825230226	25.905630204216678	31.54576034409867	22.131573626454422
39	21.569986949249124	25.17434700292263	29.096189548370493	24.15947649945775
40	20.117934672720256	25.92651140562101	33.13890778082091	20.816646140837822
41	19.976104258956305	26.965057074058418	28.991710015991767	24.06712865099351
42	22.391632814366854	26.79212543425914	30.921162435895077	19.895079315478924
43	20.96590261566458	24.634303255335183	31.1791629138099	23.220631215190338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	321.5
2	465.0
3	1590.5
4	2716.0
5	2716.0
6	4369.5
7	6023.0
8	5659.5
9	5296.0
10	6791.0
11	8286.0
12	8286.0
13	15751.5
14	23217.0
15	37120.0
16	51023.0
17	42406.5
18	33790.0
19	33790.0
20	37185.0
21	40580.0
22	27581.5
23	14583.0
24	15247.5
25	15912.0
26	15912.0
27	17474.0
28	19036.0
29	20230.5
30	21425.0
31	22527.0
32	23629.0
33	23629.0
34	25992.0
35	28355.0
36	32456.5
37	36558.0
38	41556.5
39	46555.0
40	46555.0
41	51329.0
42	56103.0
43	60747.0
44	65391.0
45	77511.5
46	89632.0
47	89632.0
48	121181.0
49	152730.0
50	162652.5
51	172575.0
52	130555.0
53	88535.0
54	88535.0
55	89627.5
56	90720.0
57	83594.0
58	76468.0
59	66309.0
60	56150.0
61	56150.0
62	48558.0
63	40966.0
64	38706.5
65	36447.0
66	31170.5
67	25894.0
68	25894.0
69	19911.5
70	13929.0
71	11637.0
72	9345.0
73	6219.5
74	3094.0
75	3094.0
76	2381.0
77	1668.0
78	1511.0
79	1354.0
80	1022.5
81	691.0
82	691.0
83	544.0
84	397.0
85	307.5
86	218.0
87	153.0
88	88.0
89	88.0
90	58.0
91	28.0
92	21.5
93	15.0
94	12.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1360075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.165632038114225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.96793112266043	32.886570933518385
2	8.871556747787954	6.949202543782248
3	2.5322364108593214	2.975299185036937
4	1.1550836489352245	1.8095832466975725
5	0.647822773824148	1.268619419275354
6	0.42675137190239243	1.0028392322213726
7	0.3023828577076368	0.8290111017727528
8	0.22888632597008488	0.7171582097200172
9	0.18460503259562921	0.6507155501122046
>10	1.3127695239342925	10.383246646086405
>50	0.1814034752963632	4.947744287549231
>100	0.15777208599592055	12.436337953066438
>500	0.017761168821377862	4.780736325997297
>1k	0.010770070455516364	7.265458205841346
>5k	0.0015115888358619458	4.4241162777792695
>10k+	7.557944179309729E-4	6.673360881543269
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	25694	1.8891605242357958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24737	1.8187967575317536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23833	1.7523298347517602	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15913	1.1700090068562394	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9420	0.6926088634817933	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	8707	0.6401852838997849	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8313	0.611216293219124	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	8021	0.5897468889583295	RNA PCR Primer, Index 39 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7972	0.5861441464625113	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	6734	0.4951197544253074	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5323	0.39137547561715347	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	5293	0.389169714905428	RNA PCR Primer, Index 39 (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4829	0.35505394923074096	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3499	0.2572652243442457	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	3379	0.2484421814973439	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3360	0.2470451997132511	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3212	0.23616344686873883	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	2695	0.1981508372700035	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2656	0.19528334834476038	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2627	0.19315111299009247	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2586	0.19013657335073433	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2531	0.186092678712571	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	2445	0.1797694980056247	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2441	0.17947539657739464	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2432	0.178813668363877	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	2092	0.15381504696432183	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	1988	0.14616840983034024	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	1933	0.1421245151921769	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1897	0.13947760233810635	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1824	0.13411025127290774	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1773	0.13036045806297447	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	1719	0.12639008878186864	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1682	0.12366965057074059	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	1643	0.12080216164549748	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1622	0.11925812914728967	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	1619	0.11903755307611712	RNA PCR Primer, Index 39 (95% over 24bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	1523	0.11197911879859566	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1500	0.1102880355862728	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	1480	0.10881752844512252	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1420	0.1044060070216716	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1394	0.10249434773817621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.352535705751521E-5	0.0
3	0.0	0.0	0.0	1.4705071411503042E-4	7.352535705751521E-5
4	0.0	0.0	0.0	2.2057607117254564E-4	7.352535705751521E-5
5	0.0	0.0	0.0	3.6762678528757603E-4	7.352535705751521E-5
6	0.0	0.0	0.0	3.6762678528757603E-4	7.352535705751521E-5
7	0.0	0.0	0.0	3.6762678528757603E-4	7.352535705751521E-5
8	0.0	0.0	0.0	4.411521423450913E-4	7.352535705751521E-5
9	0.0	0.0	0.0	7.352535705751521E-4	7.352535705751521E-5
10	0.0	0.0	0.0	8.087789276326673E-4	7.352535705751521E-5
11	0.0	0.0	0.0	8.823042846901825E-4	7.352535705751521E-5
12	0.0	0.0	0.0	8.823042846901825E-4	7.352535705751521E-5
13	0.0	0.0	0.0	8.823042846901825E-4	7.352535705751521E-5
14	0.0	0.0	0.0	9.558296417476978E-4	7.352535705751521E-5
15	0.0	0.0	0.0	0.001029354998805213	1.4705071411503042E-4
16	0.0	0.0	0.0	0.0012499310699777585	1.4705071411503042E-4
17	0.0	0.0	0.0	0.0016175578552653346	1.4705071411503042E-4
18	0.0	0.0	0.0	0.0018381339264378802	1.4705071411503042E-4
19	0.0	0.0	0.0	0.002132235354667941	1.4705071411503042E-4
20	0.0	0.0	0.0	0.002426336782898002	1.4705071411503042E-4
21	0.0	0.0	0.0	0.003823318566990791	2.2057607117254564E-4
22	0.0	0.0	0.0	0.0052938257081410955	2.9410142823006085E-4
23	0.0	0.0	0.0	0.007793687848096613	4.411521423450913E-4
24	0.0	0.0	0.0	0.01044060070216716	4.411521423450913E-4
25	0.0	0.0	0.0	0.012352259985662556	4.411521423450913E-4
26	0.0	0.0	0.0	0.016249103909710863	4.411521423450913E-4
27	0.0	0.0	0.0	0.025586824256015293	5.146774994026065E-4
28	0.0	0.0	0.0	0.046615076374464645	5.146774994026065E-4
29	0.0	0.0	0.0	0.07102549491755969	5.146774994026065E-4
30	0.0	0.0	0.0	0.1357278091281731	5.146774994026065E-4
31	0.0	0.0	0.0	0.2407220190063048	5.146774994026065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	3170	0.0	32.68139	3
TTATACA	3215	0.0	32.39658	2
CTTATAC	3255	0.0	31.827957	1
GCCGTCT	1130	0.0	25.539824	36
TACACAT	4155	0.0	25.200962	5
ATACACA	4140	0.0	25.113527	4
ACGTTTA	55	1.9029918E-5	23.545454	26
TACCGAC	55	1.9029918E-5	23.545454	33
CCGTCTT	1280	0.0	22.691406	37
CGAATAC	50	2.7024548E-4	22.2	34
ATACCGA	50	2.7024548E-4	22.2	32
GTACGAA	50	2.7024548E-4	22.2	31
ACACATC	4970	0.0	21.515089	6
TGCGACG	70	5.1030584E-6	21.142857	22
ACGAATA	45	0.0038261632	20.555555	33
CACATCT	5300	0.0	20.35	7
GCGCATA	65	6.903318E-5	19.923077	26
AACACAT	160	0.0	19.65625	5
TAGGTCG	85	1.2456512E-6	19.588236	21
ATCGTCA	85	1.2456512E-6	19.588236	10
>>END_MODULE