FastQCFastQC Report
Thu 9 Feb 2017
SRR2727469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115294
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT432971.052099801375066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT400260.9726158082508808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393540.9562864767377494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT272030.6610220314757584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152980.3717352879283959No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG138920.3375700496732433No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC106360.25845055055604776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC87970.21376358529913048No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT87830.21342339089260695TruSeq Adapter, Index 1 (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG85160.20693539756819318No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC81040.19692396217621388No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC68010.16526158276905611No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA62850.15272298892861602No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56700.13777873464204501No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA56130.1363936574154848No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG51990.12633362282257354No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT50390.12244568674801849No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48830.1186549490753273No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC45060.10949399969965694No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG43490.10567896242649978No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.10563036322556786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.10412378799667776No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC41970.10198542315567247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.10152373074681907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC60850.030.0682013
TTATACA62550.029.1326942
CTTATAC62500.029.0080011
TACACAT76650.024.0874125
GCCGTCT14950.023.75919736
ATACACA77900.023.7483964
ACACATC90900.020.8404856
ACATCTC94600.020.2991548
CACATCT95250.020.0635197
ATCTCCG97750.019.3800510
CCGTCTT20850.018.27817737
CATCTCC107750.017.821819
TCTCCGA108150.017.482211
CCCACGA108700.017.29162819
CACGAGA108550.017.26439321
GCCCACG109600.017.21715418
GAGACTA108800.017.1567124
TCCGAGC109900.017.15332213
GACTAGG109100.017.14344626
AGACTAG109250.017.0860425
Produced by FastQC (version 0.11.5)