##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4115294
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129448345610303	31.0	31.0	34.0	30.0	34.0
2	31.40551999444025	31.0	31.0	34.0	30.0	34.0
3	31.486857804083986	31.0	31.0	34.0	28.0	34.0
4	35.29739138929078	37.0	35.0	37.0	33.0	37.0
5	35.11195506323485	37.0	35.0	37.0	32.0	37.0
6	35.140911925126126	37.0	35.0	37.0	32.0	37.0
7	35.04442088463181	37.0	35.0	37.0	32.0	37.0
8	35.054838609343584	37.0	35.0	37.0	32.0	37.0
9	36.62514561535579	39.0	35.0	39.0	32.0	39.0
10	36.39374295007841	38.0	35.0	39.0	32.0	39.0
11	36.51881275068075	38.0	35.0	39.0	32.0	39.0
12	36.348860373037745	38.0	35.0	39.0	32.0	39.0
13	36.45957202571675	38.0	35.0	39.0	32.0	39.0
14	37.47166690885268	39.0	36.0	41.0	32.0	41.0
15	37.42402414019509	39.0	36.0	41.0	32.0	41.0
16	37.35703232867445	39.0	36.0	41.0	32.0	41.0
17	37.3662287554668	39.0	36.0	41.0	32.0	41.0
18	37.37760631439698	39.0	36.0	41.0	32.0	41.0
19	37.39336630627119	39.0	36.0	41.0	31.0	41.0
20	37.39954496568168	39.0	36.0	41.0	32.0	41.0
21	37.34255559870085	39.0	36.0	41.0	31.0	41.0
22	37.25242570761652	39.0	36.0	41.0	31.0	41.0
23	37.154436110761466	39.0	36.0	41.0	31.0	41.0
24	37.048584378175654	39.0	36.0	41.0	31.0	41.0
25	36.937832388159876	39.0	36.0	40.0	31.0	41.0
26	36.72606015511893	39.0	35.0	40.0	30.0	41.0
27	36.59983053458635	39.0	35.0	40.0	30.0	41.0
28	36.444341036144685	38.0	35.0	40.0	30.0	41.0
29	36.26912487904874	38.0	35.0	40.0	30.0	41.0
30	36.33098534393898	38.0	35.0	40.0	30.0	41.0
31	36.29142632336839	38.0	35.0	40.0	30.0	41.0
32	36.183077563838694	38.0	35.0	40.0	30.0	41.0
33	36.026350972737305	38.0	35.0	40.0	29.0	41.0
34	35.980705145246	38.0	35.0	40.0	29.0	41.0
35	35.841669392271854	38.0	34.0	40.0	28.0	41.0
36	35.72549397442807	38.0	34.0	40.0	28.0	41.0
37	35.58074951631645	38.0	34.0	40.0	27.0	41.0
38	35.41765278495291	38.0	34.0	40.0	27.0	41.0
39	35.25115167956408	38.0	34.0	40.0	26.0	41.0
40	35.05758713715229	38.0	33.0	40.0	25.0	41.0
41	34.86042090795943	38.0	33.0	40.0	25.0	41.0
42	34.64642599046387	38.0	33.0	40.0	24.0	41.0
43	34.37579915311032	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	5.0
15	15.0
16	29.0
17	146.0
18	307.0
19	748.0
20	1595.0
21	3249.0
22	5862.0
23	10270.0
24	16658.0
25	25754.0
26	38523.0
27	54983.0
28	74657.0
29	98624.0
30	125188.0
31	154626.0
32	188254.0
33	227319.0
34	276773.0
35	335479.0
36	418131.0
37	542962.0
38	723841.0
39	791292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0828742733812	20.15724757453538	12.925540678260166	24.834337473823258
2	18.734627465255215	23.353228226221503	32.808785957941275	25.103358350582
3	20.88251774964316	25.16901587104105	30.554973715122173	23.393492664193616
4	14.835343477282548	17.792021663579806	32.07564271228252	35.296992146855125
5	13.838014975357776	38.006737793217205	33.192233653294274	14.963013578130749
6	33.028575844155974	34.456906359545634	15.936698568802132	16.577819227496263
7	26.668106822987614	32.13332996378874	20.759343074881162	20.439220138342485
8	28.737485098270017	32.15848005027102	20.249804752710258	18.85423009874872
9	26.646504478173373	13.449585861909258	19.868106628590812	40.03580303132656
10	18.541008248742376	27.806421606815938	30.561194412841463	23.091375731600223
11	34.7902482787378	22.05050234564043	20.876540047928533	22.282709327693233
12	20.928249597720114	27.53691473804788	27.895017950114863	23.639817714117147
13	30.55609149674361	19.072051717325664	26.263907268836682	24.107949517094042
14	22.799318833599738	21.921131272759613	25.07317824680327	30.206371646837383
15	26.25717627950761	26.69155593743728	22.512510649299905	24.538757133755208
16	25.432909532101473	24.93245926050484	25.658142528820544	23.976488678573148
17	23.106830277496577	28.00120720415115	25.38729918202685	23.504663336325425
18	24.42807245363272	22.886165605665113	27.70110713839643	24.98465480230574
19	23.062896599854106	25.682296331683713	28.053499944353916	23.201307124108265
20	23.152367728769804	23.74498638493386	27.932901027241307	25.169744859055026
21	24.678577034836394	24.378962961090995	27.954892165663015	22.9875678384096
22	24.32200469759876	26.032161979192736	27.191933310232514	22.453900012975986
23	23.177566414453015	24.1046933706316	27.797333556241664	24.92040665867372
24	23.480655331065048	26.190473876228527	27.916401598524914	22.412469194181508
25	23.19411444237034	25.655080779161825	27.064603403790834	24.086201374676996
26	24.422969537534865	25.557930976498884	27.855409601355337	22.163689884610918
27	24.05555957848941	25.88894013404632	27.316784657426663	22.73871563003761
28	22.87532798385729	24.990025014008722	28.38171464784776	23.75293235428623
29	22.633935752828354	25.080127932536534	29.41629443728686	22.86964187734825
30	21.69575247843775	27.097043370412905	29.023175500948412	22.184028650200933
31	24.596177089656294	25.592266311957303	26.749048792139764	23.06250780624665
32	23.424790549593784	25.539900672953138	27.63394304270849	23.40136573474459
33	22.07431595409708	25.15623428119595	27.988814407913505	24.78063535679346
34	22.91245777336929	27.03223633597017	27.46625149989284	22.589054390767707
35	22.306863130556405	25.430844066061866	30.38361293263616	21.878679870745565
36	23.126926047081934	26.28077119156007	27.219829251567447	23.372473509790552
37	22.900818264746093	26.74056823157714	27.9204110326018	22.43820247107497
38	22.062725044674817	25.129504720683382	28.935089449259277	23.872680785382528
39	23.338162473932602	25.036315752896392	27.060423872510686	24.56509790066032
40	22.344843406084717	25.217906667178582	29.68395453641951	22.753295390317195
41	21.990992624099277	26.180097946829555	27.825691189985452	24.003218239085715
42	23.77331971907718	25.758840073151518	28.529456218680853	21.93838398909045
43	22.381900296795322	24.407782287243634	28.80919321924509	24.401124196715955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	776.0
1	1016.5
2	1257.0
3	3281.5
4	5306.0
5	5306.0
6	7924.5
7	10543.0
8	9787.0
9	9031.0
10	12126.5
11	15222.0
12	15222.0
13	27600.5
14	39979.0
15	61954.0
16	83929.0
17	72116.0
18	60303.0
19	60303.0
20	67919.0
21	75535.0
22	55934.0
23	36333.0
24	40376.0
25	44419.0
26	44419.0
27	50797.0
28	57175.0
29	62044.5
30	66914.0
31	73775.5
32	80637.0
33	80637.0
34	90918.5
35	101200.0
36	117254.5
37	133309.0
38	151018.5
39	168728.0
40	168728.0
41	186700.0
42	204672.0
43	217837.5
44	231003.0
45	257619.5
46	284236.0
47	284236.0
48	331889.5
49	379543.0
50	399101.5
51	418660.0
52	363732.5
53	308805.0
54	308805.0
55	303483.0
56	298161.0
57	282526.5
58	266892.0
59	242193.5
60	217495.0
61	217495.0
62	189257.5
63	161020.0
64	150011.0
65	139002.0
66	118516.0
67	98030.0
68	98030.0
69	75899.5
70	53769.0
71	44520.5
72	35272.0
73	23643.5
74	12015.0
75	12015.0
76	9221.5
77	6428.0
78	5644.0
79	4860.0
80	3620.5
81	2381.0
82	2381.0
83	1831.0
84	1281.0
85	1000.0
86	719.0
87	494.0
88	269.0
89	269.0
90	179.0
91	89.0
92	76.0
93	63.0
94	44.5
95	26.0
96	26.0
97	16.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4115294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.00793386098529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41196912298938	35.82772552657094
2	10.4208842258645	9.172031675696598
3	3.1546804426924675	4.164929048236618
4	1.4275432684920586	2.5129291897397312
5	0.8001946195773503	1.760745594713816
6	0.5272565845661087	1.3922083740812587
7	0.3707311504459244	1.142057836432186
8	0.27466116081725755	0.9669816159541853
9	0.20323998411952648	0.804975460113584
>10	1.181409595422545	9.586156304766313
>50	0.10669975157255203	3.2633429166428716
>100	0.09261761427501976	8.486257663827262
>500	0.014662587177628663	4.369643425455137
>1k	0.012512809349339345	10.278935424335742
>5k	5.512250814686937E-4	1.6691392605704811
>10k+	3.858575570280856E-4	4.601940682863425
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	43297	1.052099801375066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40026	0.9726158082508808	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39354	0.9562864767377494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27203	0.6610220314757584	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15298	0.3717352879283959	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	13892	0.3375700496732433	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	10636	0.25845055055604776	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8797	0.21376358529913048	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	8783	0.21342339089260695	TruSeq Adapter, Index 1 (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8516	0.20693539756819318	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	8104	0.19692396217621388	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	6801	0.16526158276905611	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	6285	0.15272298892861602	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5670	0.13777873464204501	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5613	0.1363936574154848	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	5199	0.12633362282257354	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5039	0.12244568674801849	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4883	0.1186549490753273	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	4506	0.10949399969965694	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	4349	0.10567896242649978	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4347	0.10563036322556786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4285	0.10412378799667776	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	4197	0.10198542315567247	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4178	0.10152373074681907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.429960046596914E-5	0.0
2	0.0	0.0	0.0	7.289880139790742E-5	0.0
3	0.0	0.0	0.0	7.289880139790742E-5	0.0
4	0.0	2.429960046596914E-5	0.0	1.943968037277531E-4	0.0
5	0.0	2.429960046596914E-5	0.0	3.644940069895371E-4	0.0
6	0.0	2.429960046596914E-5	0.0	3.644940069895371E-4	0.0
7	0.0	2.429960046596914E-5	0.0	3.644940069895371E-4	0.0
8	0.0	2.429960046596914E-5	0.0	3.644940069895371E-4	0.0
9	0.0	4.859920093193828E-5	0.0	5.102916097853519E-4	2.429960046596914E-5
10	0.0	4.859920093193828E-5	0.0	5.588908107172902E-4	2.429960046596914E-5
11	0.0	4.859920093193828E-5	0.0	6.317896121151976E-4	2.429960046596914E-5
12	0.0	4.859920093193828E-5	0.0	6.317896121151976E-4	7.289880139790742E-5
13	0.0	7.289880139790742E-5	0.0	6.560892125811668E-4	1.2149800232984569E-4
14	0.0	9.719840186387655E-5	0.0	6.803888130471359E-4	1.2149800232984569E-4
15	0.0	9.719840186387655E-5	0.0	7.532876144450433E-4	1.4579760279581484E-4
16	0.0	9.719840186387655E-5	0.0	9.719840186387655E-4	1.7009720326178397E-4
17	0.0	9.719840186387655E-5	0.0	0.0011906804228324877	1.7009720326178397E-4
18	0.0	9.719840186387655E-5	0.0	0.001239279623764426	1.943968037277531E-4
19	0.0	1.2149800232984569E-4	0.0	0.001433676427492179	1.943968037277531E-4
20	0.0	1.2149800232984569E-4	0.0	0.0016280732312199324	2.1869640419372224E-4
21	0.0	1.2149800232984569E-4	0.0	0.001919668436811562	2.1869640419372224E-4
22	0.0	1.2149800232984569E-4	0.0	0.002794454053586451	2.6729560512566054E-4
23	0.0	1.2149800232984569E-4	0.0	0.0040823328782828155	2.915952055916297E-4
24	0.0	1.2149800232984569E-4	0.0	0.006172098518356161	2.915952055916297E-4
25	0.0	1.2149800232984569E-4	0.0	0.007387078541654618	2.915952055916297E-4
26	0.0	1.2149800232984569E-4	0.0	0.010497427401298668	3.158948060575988E-4
27	0.0	1.2149800232984569E-4	0.0	0.020484563192811982	3.158948060575988E-4
28	0.0	1.2149800232984569E-4	0.0	0.044443969252257555	3.158948060575988E-4
29	0.0	1.2149800232984569E-4	0.0	0.07404088261980797	3.158948060575988E-4
30	0.0	1.2149800232984569E-4	0.0	0.12317467476199756	3.158948060575988E-4
31	0.0	1.4579760279581484E-4	0.0	0.2174814241704238	3.158948060575988E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	6085	0.0	30.068201	3
TTATACA	6255	0.0	29.132694	2
CTTATAC	6250	0.0	29.008001	1
TACACAT	7665	0.0	24.087412	5
GCCGTCT	1495	0.0	23.759197	36
ATACACA	7790	0.0	23.748396	4
ACACATC	9090	0.0	20.840485	6
ACATCTC	9460	0.0	20.299154	8
CACATCT	9525	0.0	20.063519	7
ATCTCCG	9775	0.0	19.38005	10
CCGTCTT	2085	0.0	18.278177	37
CATCTCC	10775	0.0	17.82181	9
TCTCCGA	10815	0.0	17.4822	11
CCCACGA	10870	0.0	17.291628	19
CACGAGA	10855	0.0	17.264393	21
GCCCACG	10960	0.0	17.217154	18
GAGACTA	10880	0.0	17.15671	24
TCCGAGC	10990	0.0	17.153322	13
GACTAGG	10910	0.0	17.143446	26
AGACTAG	10925	0.0	17.08604	25
>>END_MODULE