FastQCFastQC Report
Thu 9 Feb 2017
SRR2727467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755394
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227720.6063811147378944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197150.5249782046837163No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT163010.43406896852900123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159090.4236306496735096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.2158228936830596No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG55400.1475211389271006No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT50550.13460638217987247TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC40610.10813778793916165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC30900.024.18773
TTATACA33750.022.1451842
CTTATAC33450.022.0672651
GCCGTCT9150.021.63387936
TACACAT38200.019.6138745
GGTATCA79500.019.3842771
ATACACA42050.018.4780034
ATCTCCG39850.018.24466710
ACACATC41400.018.053146
ACATCTC42550.017.9565228
CCGTCTT11800.017.40254237
CACATCT44600.016.7993287
TGCCGTC12100.016.51239635
TATGCCG12000.016.34166733
TCTCCGA45200.016.04424911
CACGAGA45750.016.01311521
ATCTCGT44650.015.95184937
GACGTAG45000.015.91000126
AGACGTA45350.015.90959225
CCCACGA46250.015.88000119
Produced by FastQC (version 0.11.5)