##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2805966
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290917994017033	31.0	31.0	34.0	30.0	34.0
2	31.576806347617897	31.0	31.0	34.0	30.0	34.0
3	31.674543811293507	31.0	31.0	34.0	30.0	34.0
4	35.46215599191152	37.0	35.0	37.0	33.0	37.0
5	35.26968466474647	37.0	35.0	37.0	33.0	37.0
6	35.267682145827855	37.0	35.0	37.0	32.0	37.0
7	35.20702210931993	37.0	35.0	37.0	32.0	37.0
8	35.187844756493845	37.0	35.0	37.0	32.0	37.0
9	36.81398705472554	39.0	37.0	39.0	32.0	39.0
10	36.58543047207272	39.0	35.0	39.0	32.0	39.0
11	36.710846460719765	39.0	35.0	39.0	32.0	39.0
12	36.55059719184053	39.0	35.0	39.0	32.0	39.0
13	36.66657258142116	39.0	35.0	39.0	32.0	39.0
14	37.75878538799116	40.0	37.0	41.0	32.0	41.0
15	37.72579603601754	40.0	37.0	41.0	32.0	41.0
16	37.6996884495393	39.0	37.0	41.0	32.0	41.0
17	37.603344088987534	39.0	36.0	41.0	32.0	41.0
18	37.63728320300388	39.0	36.0	41.0	32.0	41.0
19	37.67517104626357	39.0	37.0	41.0	32.0	41.0
20	37.639053359876776	39.0	37.0	41.0	32.0	41.0
21	37.590664320237664	39.0	36.0	41.0	32.0	41.0
22	37.51425676576267	39.0	36.0	41.0	32.0	41.0
23	37.40267130820545	39.0	36.0	41.0	32.0	41.0
24	37.36710138326694	39.0	36.0	41.0	31.0	41.0
25	37.25092285508805	39.0	36.0	41.0	31.0	41.0
26	37.08775979466608	39.0	36.0	41.0	31.0	41.0
27	37.02282315608956	39.0	36.0	40.0	31.0	41.0
28	36.917582394084604	39.0	36.0	40.0	30.0	41.0
29	36.80569579246505	39.0	36.0	40.0	30.0	41.0
30	36.90808441727376	39.0	36.0	40.0	30.0	41.0
31	36.87562572034016	39.0	36.0	40.0	30.0	41.0
32	36.866981638409015	39.0	35.0	40.0	30.0	41.0
33	36.74512805928511	39.0	35.0	40.0	30.0	41.0
34	36.711158652670775	39.0	35.0	40.0	30.0	41.0
35	36.615614016705834	39.0	35.0	40.0	30.0	41.0
36	36.57380310381523	39.0	35.0	40.0	30.0	41.0
37	36.49415032113718	39.0	35.0	40.0	30.0	41.0
38	36.3830099865786	38.0	35.0	40.0	30.0	41.0
39	36.28588051316374	38.0	35.0	40.0	30.0	41.0
40	36.15702791837107	38.0	35.0	40.0	29.0	41.0
41	36.05223726873383	38.0	35.0	40.0	29.0	41.0
42	35.94911021730128	38.0	35.0	40.0	28.0	41.0
43	35.77535044972035	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	3.0
16	21.0
17	54.0
18	102.0
19	274.0
20	561.0
21	1160.0
22	2358.0
23	4219.0
24	7195.0
25	11579.0
26	18065.0
27	27059.0
28	39061.0
29	53232.0
30	71356.0
31	92151.0
32	114559.0
33	141478.0
34	176449.0
35	217900.0
36	274884.0
37	355655.0
38	510050.0
39	686536.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.63513242854689	20.77355890983711	12.823177472570944	25.768131189045057
2	18.016576109617866	22.510073179789064	35.038129471276555	24.435221239316512
3	19.643930111768995	24.87385805815181	31.121189636652762	24.361022193426436
4	14.17130499799356	17.749787417238842	34.63473898115657	33.44416860361102
5	13.775576753246476	37.306831230314266	34.64026292549517	14.277329090944082
6	32.31899459936435	36.308993052659936	16.394175838196187	14.977836509779518
7	26.75324647554532	31.804020433604684	21.838218994813193	19.6045140960368
8	26.58189728599705	34.18042128806978	20.74768546732213	18.489995958611043
9	25.936593672197027	14.383709567400318	20.30010342249336	39.3795933379093
10	16.638013432807096	28.119086261202025	33.51277242846136	21.73012787752952
11	34.46371053676345	22.24535151174319	22.511641267214213	20.77929668427914
12	20.22102192257497	26.82748115978597	30.016899705841055	22.934597211798007
13	29.71960458537274	20.749574299902424	25.964249032240588	23.56657208248425
14	21.800941280115296	21.958676619745216	25.94600219674793	30.29437990339156
15	24.3650849653916	28.50444374593277	22.853127942391318	24.27734334628431
16	24.762630766017836	26.49543864751034	25.26017777834799	23.481752808123833
17	22.372544784933247	28.433095768088425	25.888553175626505	23.305806271351827
18	23.5565933443242	25.467664255375865	27.109059767652212	23.866682632647723
19	22.967919069582454	26.63392927783159	27.34491437173508	23.053237280850873
20	23.038625557116514	25.70890025039505	27.376846333847237	23.875627858641195
21	24.16932350570178	25.602733604042243	27.220821635044757	23.007121255211217
22	23.507626250638815	27.1193592509674	27.07081981748888	22.3021946809049
23	23.768106954966665	25.482739277667655	26.94615686718941	23.802996900176268
24	23.686174387002552	26.698826714222484	27.100292733411596	22.51470616536337
25	23.25209927704042	26.735320385207807	26.883611561936245	23.12896877581553
26	24.131725045848736	26.567784499170692	26.927375456438174	22.37311499854239
27	23.241942347127516	27.279767466890192	26.819426892556784	22.658863293425508
28	23.34522228708402	25.90230957894714	27.39844317429363	23.354024959675208
29	22.749990555837098	27.154890686487292	27.216545032976164	22.878573724699443
30	22.68758780398622	27.03575168052642	27.541495513488044	22.73516500199931
31	24.005707838227547	25.952345823149674	27.00970004625858	23.032246292364196
32	22.84297101247841	26.941737711718535	27.083542708642945	23.131748567160116
33	23.307303082075833	26.060828962289634	27.360060670728014	23.27180728490652
34	23.989456750366898	26.191479155485137	27.13087043820203	22.688193655945938
35	22.641079756490278	25.80109666332379	28.228959296014278	23.328864284171654
36	22.556367397181575	27.00927238605172	27.14840450668326	23.285955710083446
37	23.044434608259685	26.4633641319959	27.627633406819612	22.864567852924804
38	22.351411243044286	25.616561284064026	28.52215600616686	23.509871466724828
39	23.07978785202672	25.72340505907769	27.362555355268025	23.83425173362756
40	22.444070954530453	25.503480797700327	29.196469237332167	22.855979010437046
41	22.608827049222977	25.883884551701623	27.79691557203473	23.710372827040672
42	23.10498416588084	25.63306184037868	28.31684346852385	22.945110525216627
43	22.301838297399186	25.151801554259745	28.763498916237758	23.78286123210331
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1336.0
1	1207.5
2	1079.0
3	1808.0
4	2537.0
5	2537.0
6	3382.5
7	4228.0
8	3717.5
9	3207.0
10	4581.0
11	5955.0
12	5955.0
13	10027.0
14	14099.0
15	19864.5
16	25630.0
17	24937.0
18	24244.0
19	24244.0
20	29588.5
21	34933.0
22	32448.0
23	29963.0
24	36459.0
25	42955.0
26	42955.0
27	51038.5
28	59122.0
29	65044.0
30	70966.0
31	79177.0
32	87388.0
33	87388.0
34	98148.5
35	108909.0
36	119093.5
37	129278.0
38	138807.0
39	148336.0
40	148336.0
41	158176.5
42	168017.0
43	175817.0
44	183617.0
45	191730.0
46	199843.0
47	199843.0
48	208569.0
49	217295.0
50	229200.5
51	241106.0
52	222460.0
53	203814.0
54	203814.0
55	200174.5
56	196535.0
57	182546.5
58	168558.0
59	150138.0
60	131718.0
61	131718.0
62	116515.5
63	101313.0
64	89500.0
65	77687.0
66	65081.5
67	52476.0
68	52476.0
69	41112.0
70	29748.0
71	24119.5
72	18491.0
73	13832.5
74	9174.0
75	9174.0
76	7303.0
77	5432.0
78	4341.0
79	3250.0
80	2489.0
81	1728.0
82	1728.0
83	1327.5
84	927.0
85	797.0
86	667.0
87	451.0
88	235.0
89	235.0
90	168.5
91	102.0
92	69.5
93	37.0
94	31.5
95	26.0
96	26.0
97	14.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2805966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.59059052113081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.05381246233173	52.60977757569728
2	9.492456542984948	11.882769210431999
3	2.730657100721221	5.127403213345806
4	1.1534026218168933	2.887686048325595
5	0.6679583799513831	2.0903954722347464
6	0.3948063015066046	1.4826695731657202
7	0.26689496495979376	1.1693579424765006
8	0.19165747028758168	0.9596763394468657
9	0.14918243737593467	0.8403675165667231
>10	0.8003983682164709	9.199144493010085
>50	0.061652166549617	2.6458340069163353
>100	0.032459468715218466	3.809192419722069
>500	0.0030130594254653806	1.3115842940967148
>1k	0.0012507039123679162	1.277531495074628
>5k	2.2740071133962116E-4	1.0269925635975798
>10k+	1.7055053350471585E-4	1.6796178358913647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	22405	0.7984772445567766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13653	0.4865704003541026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11145	0.3971894171205211	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9030	0.32181430566157965	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	7396	0.2635812408275795	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6283	0.22391575664138486	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	6153	0.21928277106707636	RNA PCR Primer, Index 33 (95% over 22bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	4946	0.17626728192715094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4636	0.16521939324995386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.5638350571603505E-5	0.0
2	0.0	3.5638350571603505E-5	0.0	3.5638350571603505E-5	0.0
3	3.5638350571603505E-5	3.5638350571603505E-5	0.0	7.127670114320701E-5	0.0
4	3.5638350571603505E-5	3.5638350571603505E-5	0.0	1.0691505171481051E-4	0.0
5	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	0.0
6	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	0.0
7	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	0.0
8	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	3.5638350571603505E-5
9	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	7.127670114320701E-5
10	7.127670114320701E-5	7.127670114320701E-5	0.0	1.0691505171481051E-4	7.127670114320701E-5
11	7.127670114320701E-5	7.127670114320701E-5	0.0	1.7819175285801753E-4	1.0691505171481051E-4
12	7.127670114320701E-5	7.127670114320701E-5	0.0	2.1383010342962102E-4	1.0691505171481051E-4
13	7.127670114320701E-5	7.127670114320701E-5	0.0	2.8510680457282804E-4	1.0691505171481051E-4
14	7.127670114320701E-5	7.127670114320701E-5	0.0	3.2074515514443153E-4	1.0691505171481051E-4
15	7.127670114320701E-5	7.127670114320701E-5	0.0	3.5638350571603507E-4	1.0691505171481051E-4
16	7.127670114320701E-5	7.127670114320701E-5	0.0	3.9202185628763855E-4	1.0691505171481051E-4
17	7.127670114320701E-5	7.127670114320701E-5	0.0	4.989369080024491E-4	1.0691505171481051E-4
18	7.127670114320701E-5	7.127670114320701E-5	0.0	4.989369080024491E-4	1.4255340228641402E-4
19	7.127670114320701E-5	7.127670114320701E-5	0.0	4.989369080024491E-4	1.4255340228641402E-4
20	7.127670114320701E-5	7.127670114320701E-5	0.0	4.989369080024491E-4	1.4255340228641402E-4
21	7.127670114320701E-5	7.127670114320701E-5	0.0	4.989369080024491E-4	1.4255340228641402E-4
22	7.127670114320701E-5	7.127670114320701E-5	0.0	6.771286608604665E-4	1.4255340228641402E-4
23	7.127670114320701E-5	7.127670114320701E-5	0.0	9.978738160048982E-4	1.4255340228641402E-4
24	7.127670114320701E-5	7.127670114320701E-5	0.0	0.0018175558791517787	1.4255340228641402E-4
25	7.127670114320701E-5	7.127670114320701E-5	0.0	0.0027441529940134698	1.4255340228641402E-4
26	7.127670114320701E-5	7.127670114320701E-5	0.0	0.004632985574308456	1.4255340228641402E-4
27	7.127670114320701E-5	7.127670114320701E-5	0.0	0.012045762493201984	1.4255340228641402E-4
28	7.127670114320701E-5	7.127670114320701E-5	0.0	0.027120784784990266	1.4255340228641402E-4
29	7.127670114320701E-5	7.127670114320701E-5	0.0	0.04615166399022654	1.4255340228641402E-4
30	7.127670114320701E-5	1.0691505171481051E-4	0.0	0.07348627887864642	1.7819175285801753E-4
31	7.127670114320701E-5	1.0691505171481051E-4	0.0	0.14857628353301502	1.7819175285801753E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2985	0.0	28.075377	1
TATACAC	3145	0.0	27.529411	3
GCCGTCT	1035	0.0	26.096619	36
TTATACA	3410	0.0	25.281525	2
CCGTCTT	1145	0.0	23.58952	37
GGTATCA	3945	0.0	23.259823	1
TACACAT	4085	0.0	21.239902	5
ATACACA	4510	0.0	19.894678	4
ACACATC	4690	0.0	18.697227	6
ACATCTC	4920	0.0	18.011179	8
ATGCCGT	1555	0.0	17.607716	34
TGCCGTC	1540	0.0	17.418833	35
CACATCT	5080	0.0	17.225395	7
ATCTCCG	5090	0.0	16.937132	10
TATGCCG	1690	0.0	16.201183	33
GTATCAA	6230	0.0	14.817818	2
CGCACTA	100	1.0942564E-4	14.799999	29
TCTCCGA	5865	0.0	14.667519	11
CATCTCC	6025	0.0	14.523652	9
CGAGACA	5930	0.0	14.413154	23
>>END_MODULE