FastQCFastQC Report
Thu 9 Feb 2017
SRR2727465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192332
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163780.5130418765967951No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT152340.4772060048892158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT149110.46708800964310726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113910.35682378900440176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.18882747784378318No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG48140.15079885174850233No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT44900.1406495314397124TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC39510.12376532265441065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC25800.025.240313
CTTATAC26900.023.2453541
TTATACA29350.021.9982972
GCCGTCT7800.021.10897336
TACACAT32300.019.9318895
GGTATCA63050.018.4559881
ATACACA37150.018.0269184
ACACATC35950.017.9082076
ATCTCCG35400.017.76836210
ACATCTC37100.017.7520228
CCGTCTT9850.016.71573437
CACATCT39200.016.2346947
TCTCCGA41000.015.38658611
ATCTCGT39850.015.27352537
AGACCGA41750.015.02155725
GAGACCG42100.014.9845624
GATCTCG41250.014.934545536
GACCGAG42350.014.89610426
TGCCGTC10950.014.86757935
CGAGACC42450.014.77385123
Produced by FastQC (version 0.11.5)