##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727465.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3192332
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268776555821887	31.0	31.0	34.0	30.0	34.0
2	31.553217835738888	31.0	31.0	34.0	30.0	34.0
3	31.653675119003914	31.0	31.0	34.0	30.0	34.0
4	35.44476075796628	37.0	35.0	37.0	33.0	37.0
5	35.25871337943547	37.0	35.0	37.0	33.0	37.0
6	35.25373488722351	37.0	35.0	37.0	32.0	37.0
7	35.188024616487255	37.0	35.0	37.0	32.0	37.0
8	35.17926738196403	37.0	35.0	37.0	32.0	37.0
9	36.79762787830339	39.0	37.0	39.0	32.0	39.0
10	36.563525347614224	39.0	35.0	39.0	32.0	39.0
11	36.69607233834075	39.0	35.0	39.0	32.0	39.0
12	36.52017083436184	39.0	35.0	39.0	32.0	39.0
13	36.641141021673185	39.0	35.0	39.0	32.0	39.0
14	37.72735010017755	39.0	37.0	41.0	32.0	41.0
15	37.688925525289974	39.0	37.0	41.0	32.0	41.0
16	37.6610487255085	39.0	36.0	41.0	32.0	41.0
17	37.61681209849101	39.0	36.0	41.0	32.0	41.0
18	37.61466789795046	39.0	36.0	41.0	32.0	41.0
19	37.645911515468946	39.0	37.0	41.0	32.0	41.0
20	37.61301048888399	39.0	36.0	41.0	32.0	41.0
21	37.55319528169376	39.0	36.0	41.0	32.0	41.0
22	37.472607485687575	39.0	36.0	41.0	32.0	41.0
23	37.39180761900705	39.0	36.0	41.0	32.0	41.0
24	37.32723664080052	39.0	36.0	41.0	31.0	41.0
25	37.221046871064786	39.0	36.0	41.0	31.0	41.0
26	37.0470778102027	39.0	36.0	41.0	31.0	41.0
27	36.9781019016819	39.0	36.0	40.0	31.0	41.0
28	36.84301413512129	39.0	36.0	40.0	30.0	41.0
29	36.701128203457536	39.0	35.0	40.0	30.0	41.0
30	36.806982481771946	39.0	35.0	40.0	30.0	41.0
31	36.76702642456988	39.0	35.0	40.0	30.0	41.0
32	36.752414849082115	39.0	35.0	40.0	30.0	41.0
33	36.620221205062634	39.0	35.0	40.0	30.0	41.0
34	36.58557067372692	39.0	35.0	40.0	30.0	41.0
35	36.495326300647925	39.0	35.0	40.0	30.0	41.0
36	36.42397000061397	38.0	35.0	40.0	30.0	41.0
37	36.32365023437412	38.0	35.0	40.0	30.0	41.0
38	36.21301230573762	38.0	35.0	40.0	29.0	41.0
39	36.105320185995694	38.0	35.0	40.0	29.0	41.0
40	35.9662970518104	38.0	35.0	40.0	28.0	41.0
41	35.840715502021716	38.0	34.0	40.0	28.0	41.0
42	35.72346829841007	38.0	34.0	40.0	27.0	41.0
43	35.55478753462986	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	6.0
15	13.0
16	35.0
17	70.0
18	163.0
19	349.0
20	699.0
21	1555.0
22	2966.0
23	5286.0
24	8992.0
25	14240.0
26	21988.0
27	32335.0
28	45969.0
29	62717.0
30	82945.0
31	105942.0
32	133347.0
33	163412.0
34	202716.0
35	250351.0
36	315831.0
37	410243.0
38	582653.0
39	747501.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.35873085882045	20.418803558025918	12.704255071214398	25.51821051193923
2	18.438464420367307	22.49412028573469	34.3286663166613	24.738748977236703
3	20.19962209444381	24.61861736185334	30.451845234142315	24.729915309560536
4	14.45692365330423	17.264401071066544	34.323466356256176	33.95520891937305
5	14.046189431425052	37.39210082159374	34.047429903907236	14.514279843073966
6	32.9653682637019	35.70790882652556	16.235811312858438	15.090911596914106
7	27.287324751936833	31.667195016057224	21.62018236198491	19.425297870021037
8	27.233633594500823	33.55164813684792	20.409030138469305	18.80568813018195
9	26.405868813143496	14.160995786152569	20.12334556681448	39.30978983388946
10	17.012015041042098	27.7998967525934	33.02175337652851	22.166334829835996
11	35.05659812325285	22.161980646123272	21.865457602780662	20.91596362784322
12	20.613144246901637	26.762066100894266	29.788818957426734	22.83597069477736
13	30.01849431700713	20.521706388934486	25.681508063697635	23.77829123036075
14	22.20940679102299	21.67274581716438	25.83127318837765	30.28657420343498
15	24.827210954249118	28.201421406044236	22.816392530601455	24.15497510910519
16	24.802652105106862	26.325676652678982	25.113365401844167	23.758305840369985
17	22.88327153942635	27.699092700884492	25.838383977606338	23.579251782082817
18	23.635354969345293	25.1222617196457	27.136995776128547	24.105387534880457
19	23.34478368791216	26.05051730208512	27.27730073187876	23.327398278123955
20	23.573174719922616	25.212697175607047	27.27401160029721	23.940116504173126
21	24.26987543902075	25.424924475273876	27.17139069495278	23.133809390752592
22	24.284347617979584	25.992722561437848	27.05066390337847	22.6722659172041
23	23.77124309125743	25.353440682234808	26.921197419316034	23.954118807191733
24	23.595446839489124	26.004438134880708	27.151593255338103	23.248521770292065
25	23.712351973416297	25.967036010039056	26.99174146047466	23.328870556069983
26	23.95208267811744	26.08942302993548	26.928966034861034	23.029528257086042
27	24.06657578221814	26.05737749081236	26.977050006077064	22.89899672089244
28	23.41523375388274	25.762013474788965	27.33418704570828	23.488565725620017
29	23.076672476421624	25.74769792114354	27.303300533904366	23.872329068530465
30	23.411412096235605	26.206328163862658	27.57285269827825	22.80940704162349
31	23.43268181379631	26.260708472677653	26.85362925911215	23.45298045441389
32	23.653554830763216	25.959079444117965	27.031336339704016	23.3560293854148
33	23.462628573719776	25.698580222859025	27.242529912302356	23.596261291118843
34	23.442267282976832	25.992471960936392	27.09273346255966	23.472527293527115
35	23.00271400343072	25.754244859243965	28.411800527012854	22.831240610312463
36	23.255883160022204	26.174345274864898	27.11719833651387	23.45257322859903
37	23.41307232455772	26.10925179461284	27.329989487308964	23.147686393520473
38	22.629569856769283	25.55216687988593	27.926982531892047	23.891280731452742
39	23.304562307429176	25.583147366877878	27.275170627616426	23.837119698076517
40	22.793024033841093	25.4458182920824	28.52610568073747	23.23505199333904
41	22.687615197918014	25.875754777385307	27.784954697694346	23.65167532700233
42	23.267473433214338	25.610118245846607	28.081101840284784	23.041306480654267
43	22.533151313835777	25.09272218553709	28.228110359448827	24.146016141178297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1144.0
1	1089.0
2	1034.0
3	1944.0
4	2854.0
5	2854.0
6	3884.5
7	4915.0
8	4396.0
9	3877.0
10	5542.5
11	7208.0
12	7208.0
13	12326.0
14	17444.0
15	25442.5
16	33441.0
17	31403.5
18	29366.0
19	29366.0
20	34692.0
21	40018.0
22	36245.0
23	32472.0
24	38826.0
25	45180.0
26	45180.0
27	53560.5
28	61941.0
29	68180.0
30	74419.0
31	82787.0
32	91155.0
33	91155.0
34	102215.0
35	113275.0
36	125044.5
37	136814.0
38	147806.5
39	158799.0
40	158799.0
41	171473.5
42	184148.0
43	194047.0
44	203946.0
45	215312.5
46	226679.0
47	226679.0
48	237830.0
49	248981.0
50	246959.5
51	244938.0
52	241932.0
53	238926.0
54	238926.0
55	238840.5
56	238755.0
57	217303.5
58	195852.0
59	183134.5
60	170417.0
61	170417.0
62	149652.0
63	128887.0
64	113759.0
65	98631.0
66	82735.0
67	66839.0
68	66839.0
69	52401.0
70	37963.0
71	31167.5
72	24372.0
73	17929.0
74	11486.0
75	11486.0
76	9004.0
77	6522.0
78	5311.0
79	4100.0
80	3280.0
81	2460.0
82	2460.0
83	1939.5
84	1419.0
85	1219.5
86	1020.0
87	681.0
88	342.0
89	342.0
90	252.0
91	162.0
92	116.5
93	71.0
94	60.0
95	49.0
96	49.0
97	27.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3192332.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.62792399601194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40824568433717	52.86313195355372
2	9.403676380104507	11.778654592325555
3	2.677703396130233	5.030970143301218
4	1.1175440456803258	2.7995785422025254
5	0.6360542395790886	1.9917378286850165
6	0.3753320528850875	1.4103760368812637
7	0.25527924153877296	1.1191326255794893
8	0.17935071547732273	0.8985890382035302
9	0.1389784418825442	0.7833538165773726
>10	0.7095823577473382	8.047689049318231
>50	0.05379005698916589	2.3168185090930056
>100	0.037621131997380895	4.627091733891088
>500	0.004544855944365296	2.0163723464155288
>1k	0.0020476823474010163	2.31649012507798
>5k	4.994347188782967E-5	0.18854715735849395
>10k+	1.9977388755131867E-4	1.8114665015361346
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16378	0.5130418765967951	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	15234	0.4772060048892158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14911	0.46708800964310726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11391	0.35682378900440176	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6028	0.18882747784378318	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	4814	0.15079885174850233	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	4490	0.1406495314397124	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	3951	0.12376532265441065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1325062681450426E-5
2	0.0	0.0	0.0	0.0	3.1325062681450426E-5
3	3.1325062681450426E-5	0.0	0.0	3.1325062681450426E-5	3.1325062681450426E-5
4	3.1325062681450426E-5	0.0	0.0	6.265012536290085E-5	3.1325062681450426E-5
5	3.1325062681450426E-5	0.0	0.0	1.5662531340725213E-4	3.1325062681450426E-5
6	3.1325062681450426E-5	0.0	0.0	1.5662531340725213E-4	6.265012536290085E-5
7	3.1325062681450426E-5	0.0	0.0	1.5662531340725213E-4	6.265012536290085E-5
8	3.1325062681450426E-5	6.265012536290085E-5	0.0	1.5662531340725213E-4	6.265012536290085E-5
9	3.1325062681450426E-5	1.5662531340725213E-4	0.0	1.8795037608870254E-4	9.397518804435127E-5
10	3.1325062681450426E-5	1.5662531340725213E-4	0.0	1.8795037608870254E-4	9.397518804435127E-5
11	3.1325062681450426E-5	1.5662531340725213E-4	0.0	2.506005014516034E-4	1.253002507258017E-4
12	3.1325062681450426E-5	1.5662531340725213E-4	0.0	2.506005014516034E-4	1.253002507258017E-4
13	3.1325062681450426E-5	1.5662531340725213E-4	0.0	2.8192556413305384E-4	1.253002507258017E-4
14	3.1325062681450426E-5	1.8795037608870254E-4	0.0	3.1325062681450427E-4	2.506005014516034E-4
15	3.1325062681450426E-5	1.8795037608870254E-4	0.0	3.445756894959547E-4	2.506005014516034E-4
16	3.1325062681450426E-5	1.8795037608870254E-4	0.0	4.698759402217564E-4	2.506005014516034E-4
17	3.1325062681450426E-5	1.8795037608870254E-4	0.0	6.265012536290085E-4	2.506005014516034E-4
18	3.1325062681450426E-5	1.8795037608870254E-4	0.0	6.265012536290085E-4	2.506005014516034E-4
19	3.1325062681450426E-5	1.8795037608870254E-4	0.0	7.831265670362606E-4	2.506005014516034E-4
20	3.1325062681450426E-5	1.8795037608870254E-4	0.0	7.831265670362606E-4	2.506005014516034E-4
21	3.1325062681450426E-5	1.8795037608870254E-4	0.0	9.084268177620623E-4	2.506005014516034E-4
22	3.1325062681450426E-5	1.8795037608870254E-4	0.0	0.0011590273192136657	2.8192556413305384E-4
23	6.265012536290085E-5	1.8795037608870254E-4	0.0	0.0015662531340725212	2.8192556413305384E-4
24	6.265012536290085E-5	2.506005014516034E-4	0.0	0.0025373300771974845	2.8192556413305384E-4
25	6.265012536290085E-5	2.506005014516034E-4	0.0	0.003539732083003898	2.8192556413305384E-4
26	6.265012536290085E-5	2.506005014516034E-4	0.0	0.00667223835114894	2.8192556413305384E-4
27	6.265012536290085E-5	2.506005014516034E-4	0.0	0.015881806779495366	2.8192556413305384E-4
28	6.265012536290085E-5	2.506005014516034E-4	0.0	0.032922640878204394	2.8192556413305384E-4
29	6.265012536290085E-5	2.506005014516034E-4	0.0	0.05578993663566321	2.8192556413305384E-4
30	6.265012536290085E-5	2.8192556413305384E-4	0.0	0.09184508378201264	2.8192556413305384E-4
31	6.265012536290085E-5	2.8192556413305384E-4	0.0	0.17980585979152544	2.8192556413305384E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2580	0.0	25.24031	3
CTTATAC	2690	0.0	23.245354	1
TTATACA	2935	0.0	21.998297	2
GCCGTCT	780	0.0	21.108973	36
TACACAT	3230	0.0	19.931889	5
GGTATCA	6305	0.0	18.455988	1
ATACACA	3715	0.0	18.026918	4
ACACATC	3595	0.0	17.908207	6
ATCTCCG	3540	0.0	17.768362	10
ACATCTC	3710	0.0	17.752022	8
CCGTCTT	985	0.0	16.715734	37
CACATCT	3920	0.0	16.234694	7
TCTCCGA	4100	0.0	15.386586	11
ATCTCGT	3985	0.0	15.273525	37
AGACCGA	4175	0.0	15.021557	25
GAGACCG	4210	0.0	14.98456	24
GATCTCG	4125	0.0	14.9345455	36
GACCGAG	4235	0.0	14.896104	26
TGCCGTC	1095	0.0	14.867579	35
CGAGACC	4245	0.0	14.773851	23
>>END_MODULE