FastQCFastQC Report
Thu 9 Feb 2017
SRR2727464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088355
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT89760.8247309012224872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52840.4855033513881041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.4055661985289726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35080.32232130141360127No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATG28430.26121991445805826No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC22300.20489638031708407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.18146652516871795No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC16080.14774591011204985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC13950.030.634413
CTTATAC14850.028.9023571
TTATACA14950.028.7090282
GGTATCA13250.024.9924531
TACACAT17900.023.770955
ATACACA18550.022.9380054
ACACATC20350.021.3636366
TGCCGTC2700.020.55555537
ACATCTC21900.020.3584488
ATCTCCG21700.020.03456310
TATCTCG20150.019.64764436
CGCTATC20250.019.64197533
CGCTACG20550.019.62530528
CACATCT22250.019.622477
CCCACGA21900.019.59817519
GCTACGC20900.019.562229
CTACGCT20850.019.43165430
ACGCTAC21150.019.33096927
CGAGCCC22250.019.28988815
CGAGACG21550.019.22969823
Produced by FastQC (version 0.11.5)