FastQCFastQC Report
Thu 9 Feb 2017
SRR2727463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751296
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT216370.5767873289657761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142060.3786957894018494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT119270.31794345207629576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98190.2617495393591975No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT74910.19969098679496364RNA PCR Primer, Index 23 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG72620.19358642986317262No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC59230.15789209915719793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50750.13528657829187568No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC45360.12091821066639369RNA PCR Primer, Index 23 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC32800.029.103663
CTTATAC35100.026.7222231
GGTATCA40100.026.2044891
TTATACA36800.025.8396722
TACACAT41700.022.6702635
GCCGTCT13900.022.49280536
ATACACA44150.021.7055474
CCGTCTT14800.021.2537
ACACATC50150.019.2562316
ACATCTC54900.017.7586548
ATCTCCG54100.017.54251510
TATACCG1900.017.5263165
TATGCCG18950.017.27968433
CACATCT55850.017.2578337
GTATCAA62250.016.8506032
TGCCGTC18450.016.74525835
ATGCCGT18950.016.4986834
TCTAGCG3400.016.3235328
TCTCCGA63500.015.00393811
CTAGCGG3650.014.6986329
Produced by FastQC (version 0.11.5)