##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727463.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3751296
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276762484218786	31.0	31.0	34.0	30.0	34.0
2	31.568537913297163	31.0	31.0	34.0	30.0	34.0
3	31.671337052581293	31.0	31.0	34.0	30.0	34.0
4	35.45773300747262	37.0	35.0	37.0	33.0	37.0
5	35.26575882041833	37.0	35.0	37.0	33.0	37.0
6	35.25127369314498	37.0	35.0	37.0	32.0	37.0
7	35.187756711280585	37.0	35.0	37.0	32.0	37.0
8	35.16897840106459	37.0	35.0	37.0	32.0	37.0
9	36.79746093083563	39.0	37.0	39.0	32.0	39.0
10	36.5595660806292	39.0	35.0	39.0	32.0	39.0
11	36.692061890077454	39.0	35.0	39.0	32.0	39.0
12	36.51418976268468	39.0	35.0	39.0	32.0	39.0
13	36.63454949969291	39.0	35.0	39.0	32.0	39.0
14	37.729843765994474	40.0	37.0	41.0	32.0	41.0
15	37.686860487682125	39.0	37.0	41.0	32.0	41.0
16	37.66190591198349	39.0	36.0	41.0	32.0	41.0
17	37.597519630549016	39.0	36.0	41.0	32.0	41.0
18	37.587670501074825	39.0	36.0	41.0	32.0	41.0
19	37.61544250307094	39.0	36.0	41.0	32.0	41.0
20	37.5731211293377	39.0	36.0	41.0	32.0	41.0
21	37.517821041048215	39.0	36.0	41.0	32.0	41.0
22	37.43014760765346	39.0	36.0	41.0	32.0	41.0
23	37.35799521018869	39.0	36.0	41.0	31.0	41.0
24	37.278267030914115	39.0	36.0	41.0	31.0	41.0
25	37.16943504324905	39.0	36.0	41.0	31.0	41.0
26	36.991945716893575	39.0	36.0	40.0	31.0	41.0
27	36.91234682627017	39.0	36.0	40.0	30.0	41.0
28	36.788256645170094	39.0	36.0	40.0	30.0	41.0
29	36.6512357329307	39.0	35.0	40.0	30.0	41.0
30	36.78189857585219	39.0	35.0	40.0	30.0	41.0
31	36.76344681944587	39.0	35.0	40.0	30.0	41.0
32	36.75148268758317	39.0	35.0	40.0	30.0	41.0
33	36.61827459096803	39.0	35.0	40.0	30.0	41.0
34	36.58276313039547	39.0	35.0	40.0	30.0	41.0
35	36.50482846461596	39.0	35.0	40.0	30.0	41.0
36	36.430100957109225	38.0	35.0	40.0	30.0	41.0
37	36.33936271624527	38.0	35.0	40.0	30.0	41.0
38	36.224211312570375	38.0	35.0	40.0	29.0	41.0
39	36.131772059576214	38.0	35.0	40.0	29.0	41.0
40	35.99734278500017	38.0	35.0	40.0	28.0	41.0
41	35.88417069727369	38.0	34.0	40.0	28.0	41.0
42	35.78166425683284	38.0	34.0	40.0	28.0	41.0
43	35.63375110895008	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	2.0
14	1.0
15	15.0
16	31.0
17	96.0
18	175.0
19	387.0
20	956.0
21	1853.0
22	3611.0
23	6315.0
24	10931.0
25	17712.0
26	26301.0
27	38393.0
28	54533.0
29	74616.0
30	98175.0
31	125320.0
32	155571.0
33	191869.0
34	236854.0
35	293405.0
36	368632.0
37	474465.0
38	685257.0
39	885816.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.214449619544816	20.063412751219843	12.609748737502985	26.11238889173235
2	18.625616320333027	21.979897080902173	34.122367309857715	25.272119288907085
3	19.92130186474221	24.172312715392227	30.563064071723478	25.343321348142084
4	14.581174079571433	17.01188069403214	34.08456704029754	34.32237818609888
5	14.275333111543317	36.972902165011774	34.13188402088238	14.619880702562526
6	33.569358429726684	35.50636899887399	16.01982354898147	14.90444902241785
7	27.7290035230491	31.401094448425294	21.394019560173337	19.47588246835227
8	27.497670138533458	33.31917822533866	20.374078718394923	18.809072917732962
9	26.51872846077729	14.111576372538984	19.61564749889105	39.75404766779268
10	17.06495568464872	27.12481233152489	32.93205868052001	22.878173303306376
11	35.56434896099908	21.701566605247894	21.80187860408776	20.932205829665268
12	20.808675188521512	26.035802026819532	29.4798384345037	23.67568435015525
13	30.10644854471628	20.055468829972362	25.567217303033406	24.270865322277952
14	22.405296729450303	21.191742800354866	25.24359581328693	31.159364656907908
15	25.02556449824274	28.033005126761523	22.187052154775312	24.754378220220424
16	25.458561521138297	25.87929611526257	24.491242493260994	24.170899870338143
17	23.307838144470605	27.45664431705736	25.090715315453643	24.14480222301839
18	24.287366286211483	24.819742297062135	26.35827724605043	24.534614170675948
19	23.890969947452827	25.694000153546938	26.371499343160338	24.0435305558399
20	23.714177713515543	25.025058006619577	26.451578334527586	24.80918594533729
21	24.858662179684035	24.87348372402498	26.437929718156074	23.82992437813492
22	24.41353601528645	25.978221926502197	26.29325971610878	23.31498234210257
23	24.459680067901868	24.724441899546186	26.31551869007404	24.500359342477907
24	24.102416871395913	25.846720706657113	26.332072968915277	23.7187894530317
25	24.44333904869144	25.633647678029142	26.162824794417716	23.760188478861703
26	24.79756329545842	25.482606544511547	26.275265934759613	23.444564225270415
27	24.486177577029377	26.240238040399905	25.917549561538202	23.356034821032516
28	24.17407210734637	25.14027152216194	26.52011464837752	24.16554172211417
29	23.430782321629646	25.70847515098782	26.450778610912067	24.409963916470467
30	23.75165809362951	26.147896620261367	26.633995291227354	23.46644999488177
31	24.57481361108268	25.289926468079297	26.272733476643808	23.86252644419422
32	24.128621148531067	25.997441950728494	25.98384664926468	23.89009025147576
33	24.10108399870338	25.110095284403045	26.52832514416351	24.260495572730065
34	24.26252153922271	26.116307537448392	26.26502947248098	23.356141450847918
35	24.256097092844715	25.017407317364455	27.40804777868768	23.318447811103148
36	24.19145276725697	25.529630293104038	26.28715515917699	23.991761780462006
37	24.134539103285903	25.489377537789608	26.75323408230116	23.62284927662333
38	23.429822653291023	24.799216057597164	27.31045484014058	24.460506448971238
39	24.034920198246155	25.128089065752206	26.36211591940492	24.474874816596717
40	23.533813380762275	24.772718548469648	28.069712440713822	23.62375563005425
41	23.342466177022555	25.360248831337223	26.97878279933122	24.318502192309005
42	24.107748362166035	25.02948314395878	27.31647942471082	23.546289069164363
43	23.252923789538336	24.422652864503362	27.568312391237587	24.756110954720715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1043.0
1	1087.0
2	1131.0
3	1985.5
4	2840.0
5	2840.0
6	3735.0
7	4630.0
8	4078.0
9	3526.0
10	4933.0
11	6340.0
12	6340.0
13	10498.0
14	14656.0
15	20586.0
16	26516.0
17	25908.5
18	25301.0
19	25301.0
20	30635.5
21	35970.0
22	34005.5
23	32041.0
24	38658.0
25	45275.0
26	45275.0
27	53704.5
28	62134.0
29	69076.5
30	76019.0
31	86301.5
32	96584.0
33	96584.0
34	110083.0
35	123582.0
36	139203.5
37	154825.0
38	167798.5
39	180772.0
40	180772.0
41	196206.0
42	211640.0
43	223144.0
44	234648.0
45	250399.0
46	266150.0
47	266150.0
48	281805.0
49	297460.0
50	300780.0
51	304100.0
52	316353.5
53	328607.0
54	328607.0
55	301453.5
56	274300.0
57	264967.0
58	255634.0
59	232345.5
60	209057.0
61	209057.0
62	182587.0
63	156117.0
64	140544.0
65	124971.0
66	104717.0
67	84463.0
68	84463.0
69	66279.0
70	48095.0
71	39110.5
72	30126.0
73	22170.0
74	14214.0
75	14214.0
76	10921.0
77	7628.0
78	6297.0
79	4966.0
80	3854.0
81	2742.0
82	2742.0
83	2108.0
84	1474.0
85	1243.0
86	1012.0
87	693.5
88	375.0
89	375.0
90	283.0
91	191.0
92	129.5
93	68.0
94	63.5
95	59.0
96	59.0
97	35.0
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3751296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.30126131523925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57084265036606	40.82084591880214
2	10.794514282622886	11.07544395967838
3	3.584953606441425	5.5173792530118275
4	1.7271989342954936	3.544299354867835
5	1.01699465774632	2.6086554346623134
6	0.6496778683216768	1.9997576456098762
7	0.45225988928256866	1.6241051933740358
8	0.34276922132010057	1.4067594715010845
9	0.25477140586608593	1.1763085021188258
>10	1.4253988482940303	13.474079886687129
>50	0.11019592186249334	3.9085226019515575
>100	0.061327404792666025	5.948815924715242
>500	0.0053532389004388725	1.9131966493160413
>1k	0.003326284344659779	2.759743826349932
>5k	2.5986596442654516E-4	0.9483994773515007
>10k+	1.559195786559271E-4	1.2736869000022826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	21637	0.5767873289657761	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14206	0.3786957894018494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11927	0.31794345207629576	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9819	0.2617495393591975	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	7491	0.19969098679496364	RNA PCR Primer, Index 23 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG	7262	0.19358642986317262	No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC	5923	0.15789209915719793	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5075	0.13528657829187568	No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC	4536	0.12091821066639369	RNA PCR Primer, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5994472310369536E-4
2	0.0	0.0	0.0	0.0	1.5994472310369536E-4
3	0.0	0.0	0.0	2.6657453850615895E-5	1.5994472310369536E-4
4	0.0	0.0	0.0	7.997236155184768E-5	1.5994472310369536E-4
5	0.0	0.0	0.0	1.0662981540246358E-4	1.5994472310369536E-4
6	0.0	0.0	0.0	1.0662981540246358E-4	2.9323199235677485E-4
7	0.0	0.0	0.0	1.0662981540246358E-4	2.9323199235677485E-4
8	0.0	0.0	0.0	1.0662981540246358E-4	2.9323199235677485E-4
9	0.0	0.0	0.0	1.3328726925307947E-4	3.465469000580066E-4
10	0.0	0.0	0.0	2.3991708465554305E-4	3.732043539086225E-4
11	0.0	0.0	0.0	2.6657453850615893E-4	4.265192616098543E-4
12	0.0	0.0	0.0	2.9323199235677485E-4	6.664363462653974E-4
13	0.0	0.0	0.0	3.198894462073907E-4	9.330108847715563E-4
14	0.0	0.0	0.0	3.198894462073907E-4	9.596683386221722E-4
15	0.0	0.0	0.0	3.198894462073907E-4	0.0011462705155764834
16	2.6657453850615895E-5	0.0	0.0	3.465469000580066E-4	0.001252900330978947
17	2.6657453850615895E-5	0.0	0.0	3.465469000580066E-4	0.0012795577848295628
18	2.6657453850615895E-5	0.0	0.0	3.732043539086225E-4	0.0014395025079332582
19	2.6657453850615895E-5	0.0	0.0	3.732043539086225E-4	0.0014395025079332582
20	5.331490770123179E-5	0.0	0.0	4.798341693110861E-4	0.0016527621387381853
21	5.331490770123179E-5	0.0	0.0	5.598065308629337E-4	0.0017060770464394173
22	5.331490770123179E-5	0.0	0.0	6.664363462653974E-4	0.001759391954140649
23	5.331490770123179E-5	0.0	0.0	8.263810693690927E-4	0.0018660217695431127
24	5.331490770123179E-5	0.0	0.0	0.0013062152386801788	0.0019459941310949603
25	5.331490770123179E-5	0.0	0.0	0.001759391954140649	0.001999309038796192
26	5.331490770123179E-5	0.0	0.0	0.003038949738970212	0.0021059388541986557
27	5.331490770123179E-5	0.0	0.0	0.0076773467089773775	0.002212568669601119
28	5.331490770123179E-5	0.0	0.0	0.01887347732623605	0.002239226123451735
29	5.331490770123179E-5	0.0	0.0	0.03313521513631556	0.002265883577302351
30	5.331490770123179E-5	0.0	0.0	0.05368811205514041	0.002265883577302351
31	5.331490770123179E-5	0.0	0.0	0.11281434469580646	0.002265883577302351
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	3280	0.0	29.10366	3
CTTATAC	3510	0.0	26.722223	1
GGTATCA	4010	0.0	26.204489	1
TTATACA	3680	0.0	25.839672	2
TACACAT	4170	0.0	22.670263	5
GCCGTCT	1390	0.0	22.492805	36
ATACACA	4415	0.0	21.705547	4
CCGTCTT	1480	0.0	21.25	37
ACACATC	5015	0.0	19.256231	6
ACATCTC	5490	0.0	17.758654	8
ATCTCCG	5410	0.0	17.542515	10
TATACCG	190	0.0	17.526316	5
TATGCCG	1895	0.0	17.279684	33
CACATCT	5585	0.0	17.257833	7
GTATCAA	6225	0.0	16.850603	2
TGCCGTC	1845	0.0	16.745258	35
ATGCCGT	1895	0.0	16.49868	34
TCTAGCG	340	0.0	16.32353	28
TCTCCGA	6350	0.0	15.003938	11
CTAGCGG	365	0.0	14.69863	29
>>END_MODULE