FastQCFastQC Report
Thu 9 Feb 2017
SRR2727462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013380
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT267870.6674424051547574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164220.40918128858966757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136900.3411089904270216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112050.27919110575126205No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT90990.22671663286307303RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG86760.2161768883086077No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC69940.17426707662867708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56030.13960801120252753No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC54500.13579576317218903RNA PCR Primer, Index 39 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC39100.028.6726343
CTTATAC39800.027.8429641
GGTATCA41350.026.4860951
TTATACA42400.026.4410382
TACACAT49950.022.8888915
GCCGTCT14800.022.536
ATACACA52000.021.7730774
CCGTCTT16900.019.70414437
ACACATC59650.019.2288346
ATCTCCG61250.018.51510210
CACATCT63400.018.0623027
GTATCAA66000.016.6500022
TGCCGTC20150.016.52605435
ATGCCGT20350.016.27272834
TATGCCG20900.016.19856533
TCTCCGA72800.015.67925911
CGAGACC72450.015.4741223
CATCTCC74550.015.46009359
CACGAGA72400.015.433701521
CCCACGA73850.015.25592419
Produced by FastQC (version 0.11.5)