##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727462.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4013380
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265199407980305	31.0	31.0	34.0	30.0	34.0
2	31.558273076558912	31.0	31.0	34.0	30.0	34.0
3	31.659857028240534	31.0	31.0	34.0	30.0	34.0
4	35.44665892589289	37.0	35.0	37.0	33.0	37.0
5	35.25545176384992	37.0	35.0	37.0	33.0	37.0
6	35.23548081666824	37.0	35.0	37.0	32.0	37.0
7	35.17286252485436	37.0	35.0	37.0	32.0	37.0
8	35.16286272418759	37.0	35.0	37.0	32.0	37.0
9	36.78441986554974	39.0	37.0	39.0	32.0	39.0
10	36.54937633615556	39.0	35.0	39.0	32.0	39.0
11	36.67924019155924	39.0	35.0	39.0	32.0	39.0
12	36.50264739446551	39.0	35.0	39.0	32.0	39.0
13	36.62662045457943	39.0	35.0	39.0	32.0	39.0
14	37.71778650414364	39.0	37.0	41.0	32.0	41.0
15	37.67286700985205	39.0	36.0	41.0	32.0	41.0
16	37.64290672699819	39.0	36.0	41.0	32.0	41.0
17	37.58807713199348	39.0	36.0	41.0	32.0	41.0
18	37.581933183501185	39.0	36.0	41.0	32.0	41.0
19	37.61220891119206	39.0	36.0	41.0	32.0	41.0
20	37.56559882194061	39.0	36.0	41.0	32.0	41.0
21	37.50918378025505	39.0	36.0	41.0	32.0	41.0
22	37.42414324085932	39.0	36.0	41.0	32.0	41.0
23	37.3521789613742	39.0	36.0	41.0	31.0	41.0
24	37.28841051682123	39.0	36.0	41.0	31.0	41.0
25	37.16403953774624	39.0	36.0	41.0	31.0	41.0
26	36.997705923685274	39.0	36.0	41.0	31.0	41.0
27	36.92671314453154	39.0	36.0	40.0	30.0	41.0
28	36.79963621685462	39.0	36.0	40.0	30.0	41.0
29	36.6703750953062	39.0	35.0	40.0	30.0	41.0
30	36.803984920441124	39.0	35.0	40.0	30.0	41.0
31	36.776328182230436	39.0	35.0	40.0	30.0	41.0
32	36.764566524973965	39.0	35.0	40.0	30.0	41.0
33	36.63791666874305	39.0	35.0	40.0	30.0	41.0
34	36.619849353911164	39.0	35.0	40.0	30.0	41.0
35	36.537698398855824	39.0	35.0	40.0	30.0	41.0
36	36.45482187084203	39.0	35.0	40.0	30.0	41.0
37	36.3894041929745	38.0	35.0	40.0	30.0	41.0
38	36.28900178901574	38.0	35.0	40.0	30.0	41.0
39	36.1750036129148	38.0	35.0	40.0	29.0	41.0
40	36.0533298616129	38.0	35.0	40.0	29.0	41.0
41	35.94095998883734	38.0	35.0	40.0	28.0	41.0
42	35.8370445858603	38.0	34.0	40.0	28.0	41.0
43	35.69618351613852	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	6.0
15	13.0
16	46.0
17	90.0
18	146.0
19	373.0
20	954.0
21	1944.0
22	3702.0
23	6696.0
24	11249.0
25	18172.0
26	27803.0
27	41067.0
28	58245.0
29	80212.0
30	104943.0
31	134469.0
32	167430.0
33	204945.0
34	253695.0
35	313546.0
36	392333.0
37	506187.0
38	727845.0
39	957266.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.22091603586005	19.76712895365004	12.637402887341842	26.37455212314807
2	18.609800218269886	21.90916384693201	34.305547942133565	25.175487992664536
3	19.923381289586334	24.083042223761517	30.765763521022183	25.22781296562997
4	14.819105093462367	16.919454424948547	33.787156959968904	34.47428352162018
5	14.354284916952793	37.01040021129322	33.9326203848128	14.702694486941182
6	33.640372952473975	35.12876926680254	16.072138696061675	15.158719084661806
7	27.60996965151568	31.590479844918747	21.19131505115389	19.608235452411684
8	27.48725513158485	33.542051836606554	20.330195496065663	18.640497535742938
9	26.91305084492373	14.020202422895414	19.348952753041075	39.71779397913978
10	17.04797452521316	27.27237390927348	32.79437780623813	22.885273759275222
11	35.60260927198521	21.59862759070908	21.665802889335172	21.13296024797054
12	20.85264789279859	25.72083879423329	29.3862280671155	24.040285245852623
13	30.1806457399997	19.940648530664927	25.4773034200599	24.401402309275475
14	22.447263902246984	21.087711604682337	25.067100548664715	31.397923944405964
15	25.297355346366405	27.776736815352642	22.159999800666768	24.76590803761418
16	25.45761926356338	25.789633675355933	24.54898364969178	24.203763411388906
17	23.415450318684	27.27780573980037	24.854785741694034	24.451958199821597
18	24.365298077929324	24.963522018846955	26.16933856250841	24.50184134071531
19	24.07150581305533	25.564063208567344	26.312509655203343	24.051921323173982
20	23.673063602250473	24.449690784326428	26.568627939542232	25.308617673880867
21	24.487663764707055	24.943588695812508	26.619557579895247	23.949189959585187
22	24.573601303639325	25.298775595632613	26.49881147561407	23.628811625113993
23	24.05947106927328	24.60163253915652	26.298830412270952	25.040065979299243
24	23.949065376316224	25.165546247801107	26.86139862161071	24.02398975427196
25	24.048009408528472	25.47511075452611	26.13709641250019	24.33978342444523
26	24.36793924323139	25.170006328830063	26.407990272538363	24.054064155400187
27	24.43513945851128	25.896476286820587	26.293797248204754	23.37458700646338
28	23.559717744145832	25.176210575624538	26.182843388864246	25.08122829136538
29	23.346481020984804	25.308393423996733	26.944096995549888	24.40102855946858
30	23.734209070658647	25.26705669535404	27.225530600142523	23.77320363384479
31	23.909223646901115	24.925848038311845	26.466793575489987	24.69813473929705
32	24.035027831902287	25.300569594705706	26.730013106159895	23.93438946723211
33	23.249081821307726	24.805101934030667	26.411976937145248	25.53383930751636
34	24.159262267714496	25.47144800641853	27.20280661188325	23.166483113983723
35	23.69678425666147	25.486298332079194	27.280471821756226	23.536445589503113
36	23.48808236449078	25.415036702231035	26.401188026052854	24.69569290722533
37	24.037195580782285	25.553573297320465	26.90906418031684	23.500166941580414
38	23.754964643268266	24.50707383801185	27.16373729873573	24.574224219984153
39	23.940070464296927	25.09959186521087	26.526444044670576	24.433893625821625
40	23.896441403505275	24.351294918497626	28.03674209768325	23.71552158031385
41	23.362676845950297	25.3415574901953	26.86650653563829	24.429259128216117
42	24.157543018602773	24.923082289740815	27.477537636605554	23.441837055050854
43	23.166233947445793	24.364874494814845	27.52744569415306	24.941445863586303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1452.0
1	1517.5
2	1583.0
3	2572.0
4	3561.0
5	3561.0
6	4576.5
7	5592.0
8	4880.5
9	4169.0
10	5688.5
11	7208.0
12	7208.0
13	11775.5
14	16343.0
15	22654.0
16	28965.0
17	27852.5
18	26740.0
19	26740.0
20	32284.0
21	37828.0
22	34274.5
23	30721.0
24	36578.5
25	42436.0
26	42436.0
27	50721.5
28	59007.0
29	66674.5
30	74342.0
31	85399.5
32	96457.0
33	96457.0
34	109515.5
35	122574.0
36	138857.5
37	155141.0
38	170814.0
39	186487.0
40	186487.0
41	203719.5
42	220952.0
43	237410.5
44	253869.0
45	274864.5
46	295860.0
47	295860.0
48	318554.0
49	341248.0
50	359818.0
51	378388.0
52	357218.0
53	336048.0
54	336048.0
55	325410.5
56	314773.0
57	291992.5
58	269212.0
59	241100.5
60	212989.0
61	212989.0
62	186051.0
63	159113.0
64	143068.5
65	127024.0
66	106914.0
67	86804.0
68	86804.0
69	68155.0
70	49506.0
71	40572.0
72	31638.0
73	23387.5
74	15137.0
75	15137.0
76	11792.0
77	8447.0
78	6873.5
79	5300.0
80	4182.0
81	3064.0
82	3064.0
83	2351.5
84	1639.0
85	1357.0
86	1075.0
87	720.5
88	366.0
89	366.0
90	281.5
91	197.0
92	130.5
93	64.0
94	58.5
95	53.0
96	53.0
97	29.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4013380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.18124329355998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77109009651775	43.22096840311921
2	10.967056165591973	11.884174766441513
3	3.648035010014862	5.929652172631192
4	1.7266320299966391	3.7420428038280504
5	0.9295042349467347	2.5180847548021683
6	0.6316807078573221	2.0535147669759453
7	0.42448799842082807	1.609950126234463
8	0.31300463778669907	1.3567184345547008
9	0.23562727256281105	1.1489920723191271
>10	1.2264978183628172	11.786984801682436
>50	0.07094712857702294	2.647483207951318
>100	0.04690833088361106	4.938566018268466
>500	0.005043777116188666	1.943802375261499
>1k	0.003072118703417828	2.6371834237452925
>5k	2.292625898072999E-4	0.8899425786174889
>10k+	1.834100718458399E-4	1.6919392935672344
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	26787	0.6674424051547574	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16422	0.40918128858966757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13690	0.3411089904270216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11205	0.27919110575126205	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	9099	0.22671663286307303	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	8676	0.2161768883086077	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	6994	0.17426707662867708	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5603	0.13960801120252753	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	5450	0.13579576317218903	RNA PCR Primer, Index 39 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.4916653793062206E-5	0.0
5	0.0	0.0	0.0	4.983330758612441E-5	0.0
6	0.0	0.0	0.0	4.983330758612441E-5	2.4916653793062206E-5
7	0.0	0.0	0.0	7.474996137918663E-5	2.4916653793062206E-5
8	0.0	0.0	0.0	7.474996137918663E-5	4.983330758612441E-5
9	0.0	0.0	0.0	1.7441657655143544E-4	1.2458326896531104E-4
10	0.0	0.0	0.0	1.9933323034449765E-4	1.2458326896531104E-4
11	0.0	0.0	0.0	2.2424988413755986E-4	1.2458326896531104E-4
12	0.0	0.0	0.0	2.491665379306221E-4	1.9933323034449765E-4
13	0.0	0.0	0.0	2.491665379306221E-4	2.2424988413755986E-4
14	0.0	0.0	0.0	2.491665379306221E-4	2.989998455167465E-4
15	0.0	0.0	0.0	2.740831917236843E-4	4.7341642206818194E-4
16	0.0	0.0	0.0	2.989998455167465E-4	5.481663834473686E-4
17	0.0	0.0	0.0	3.488331531028709E-4	5.481663834473686E-4
18	0.0	0.0	0.0	3.488331531028709E-4	6.229163448265552E-4
19	0.0	0.0	0.0	3.488331531028709E-4	6.229163448265552E-4
20	0.0	0.0	0.0	3.737498068959331E-4	6.976663062057417E-4
21	0.0	0.0	0.0	4.4849976827511973E-4	7.474996137918662E-4
22	0.0	0.0	0.0	5.97999691033493E-4	7.474996137918662E-4
23	0.0	0.0	0.0	7.474996137918662E-4	7.973329213779906E-4
24	0.0	2.4916653793062206E-5	0.0	0.0011212494206877992	7.973329213779906E-4
25	0.0	2.4916653793062206E-5	0.0	0.001793999073100479	7.973329213779906E-4
26	0.0	2.4916653793062206E-5	0.0	0.00338866491585646	8.222495751710529E-4
27	0.0	2.4916653793062206E-5	0.0	0.010091244786190194	8.47166228964115E-4
28	0.0	2.4916653793062206E-5	0.0	0.022549571682721297	8.47166228964115E-4
29	0.0	2.4916653793062206E-5	0.0	0.039318479685452164	8.47166228964115E-4
30	0.0	2.4916653793062206E-5	0.0	0.06430988343989355	8.47166228964115E-4
31	0.0	2.4916653793062206E-5	0.0	0.1375399289377034	8.47166228964115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	3910	0.0	28.672634	3
CTTATAC	3980	0.0	27.842964	1
GGTATCA	4135	0.0	26.486095	1
TTATACA	4240	0.0	26.441038	2
TACACAT	4995	0.0	22.888891	5
GCCGTCT	1480	0.0	22.5	36
ATACACA	5200	0.0	21.773077	4
CCGTCTT	1690	0.0	19.704144	37
ACACATC	5965	0.0	19.228834	6
ATCTCCG	6125	0.0	18.515102	10
CACATCT	6340	0.0	18.062302	7
GTATCAA	6600	0.0	16.650002	2
TGCCGTC	2015	0.0	16.526054	35
ATGCCGT	2035	0.0	16.272728	34
TATGCCG	2090	0.0	16.198565	33
TCTCCGA	7280	0.0	15.679259	11
CGAGACC	7245	0.0	15.47412	23
CATCTCC	7455	0.0	15.4600935	9
CACGAGA	7240	0.0	15.4337015	21
CCCACGA	7385	0.0	15.255924	19
>>END_MODULE